Q99K82 · SMOX_MOUSE
- ProteinSpermine oxidase
- GeneSmox
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids555 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N1-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal.
Catalytic activity
- H2O + O2 + spermine = 3-aminopropanal + H2O2 + spermidine
Cofactor
Note: Binds 1 FAD per subunit.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
150 μM | spermine |
Pathway
Amine and polyamine degradation; spermine degradation.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 35 | FAD (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 55 | FAD (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 63 | FAD (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 79-80 | FAD (UniProtKB | ChEBI) | ||||
Sequence: TW | ||||||
Binding site | 261 | FAD (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Binding site | 519 | FAD (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 528-529 | FAD (UniProtKB | ChEBI) | ||||
Sequence: TT |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | nuclear membrane | |
Cellular Component | nucleoplasm | |
Molecular Function | N1-acetylspermine:oxygen oxidoreductase (N1-acetylspermidine-forming) activity | |
Molecular Function | norspermine:oxygen oxidoreductase activity | |
Molecular Function | polyamine oxidase activity | |
Molecular Function | spermine:oxygen oxidoreductase (spermidine-forming) activity | |
Biological Process | spermine catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSpermine oxidase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ99K82
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 320 | No change in enzymatic activity. | ||||
Sequence: C → R |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 17 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000099878 | 1-555 | Spermine oxidase | |||
Sequence: MQSCESSGDSADDPLSRGLRRRGQPRVVVIGAGLAGLAAARALLEQGFTDVTVLEASSHIGGRVQSVRLGDTTFELGATWIHGSHGNPIYQLAEANGLLEETTDGERSVGRISLYSKNGVACYLTNRGCRIPKDVVEEFSDLYNEVYNMTQEFFRHGKPVNAESQNSVGVFTREKVRNRIRDDPDDTEATKRLKLAMIQQYLKVESCESSSHSIDEVSLSAFGEWTEIPGAHHIIPSGFMRVVELLAEGIPPHVIQLGKPVRCIHWDQASAHPRGPEIEPRGEGDHNHDTGEGGQSGENPQQGRWDEDEPWPVVVECEDCEVIPADHVIVTVSLGVLKRQYTSFFRPCLPTEKVAAIHRLGIGTTDKIFLEFEEPFWGPECNSLQFVWEDEAESCTLTYPPELWYRKICGFDVLYPPERYGHVLSGWICGEEALVMERCDDEAVAEICTEMLRQFTGNPNIPKPRRILRSAWGSNPYFRGSYSYTQVGSSGADVEKLAKPLPYTESSKTAPMQVLFSGEATHRKYYSTTHGALLSGQREAARLIEMYRDLFQQGP |
Proteomic databases
PTM databases
Expression
Tissue specificity
Widely expressed. Isoform 1 and isoform 2 are expressed at higher level in brain and skeletal muscle. Isoform 7 is found in brain and spleen, isoform 10 is widely expressed but found at lower level in heart, kidney, liver and lung.
Induction
By antitumor polyamine analogs.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 271-307 | Disordered | ||||
Sequence: AHPRGPEIEPRGEGDHNHDTGEGGQSGENPQQGRWDE | ||||||
Compositional bias | 272-292 | Basic and acidic residues | ||||
Sequence: HPRGPEIEPRGEGDHNHDTGE |
Sequence similarities
Belongs to the flavin monoamine oxidase family.
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 10 isoforms produced by Alternative splicing.
Q99K82-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- SynonymsAlpha, Polyamine oxidase-l
- NoteMajor isoform.
- Length555
- Mass (Da)61,852
- Last updated2001-06-01 v1
- ChecksumA297E9DBD094EA74
Q99K82-2
- Name2
- SynonymsMu
- NoteActive. Nuclear and cytoplasmic.
- Differences from canonical
- 510-510: A → AHRSSTEQQPGHLLPSKCPEQSLDPSRGSIK
Q99K82-3
- Name3
- SynonymsEta
- Differences from canonical
- 205-555: ESCESSSHSIDEVSLSAFGEWTEIPGAHHIIPSGFMRVVELLAEGIPPHVIQLGKPVRCIHWDQASAHPRGPEIEPRGEGDHNHDTGEGGQSGENPQQGRWDEDEPWPVVVECEDCEVIPADHVIVTVSLGVLKRQYTSFFRPCLPTEKVAAIHRLGIGTTDKIFLEFEEPFWGPECNSLQFVWEDEAESCTLTYPPELWYRKICGFDVLYPPERYGHVLSGWICGEEALVMERCDDEAVAEICTEMLRQFTGNPNIPKPRRILRSAWGSNPYFRGSYSYTQVGSSGADVEKLAKPLPYTESSKTAPMQVLFSGEATHRKYYSTTHGALLSGQREAARLIEMYRDLFQQGP → SLLWSIDARVKKMNSGGVSVQSALLLRALVPCMA
Q99K82-4
- Name4
- SynonymsOmega
Q99K82-5
- Name5
- SynonymsPhi
- Differences from canonical
- 204-510: Missing
Q99K82-6
- Name6
- SynonymsBeta
Q99K82-7
- Name7
- SynonymsGamma
- NoteNo detectable activity. Cytoplasmic.
Q99K82-8
- Name8
- SynonymsPolyamine oxidase-m
- Differences from canonical
- 282-417: Missing
Q99K82-9
- Name9
- SynonymsPolyamine oxidase-s
Q99K82-10
- Name10
- SynonymsDelta
- NoteNo detectable activity. Cytoplasmic.
- Differences from canonical
- 146-510: Missing
Computationally mapped potential isoform sequences
There are 6 potential isoforms mapped to this entry
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_011127 | 146-510 | in isoform 10 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_011128 | 204-209 | in isoform 4 | |||
Sequence: VESCES → SAMAMC | ||||||
Alternative sequence | VSP_011129 | 204-243 | in isoform 9 | |||
Sequence: VESCESSSHSIDEVSLSAFGEWTEIPGAHHIIPSGFMRVV → GTPIYQNLGESCAQPGAATHTSGVPIPTHRWAQVGRMWRS | ||||||
Alternative sequence | VSP_011130 | 204-510 | in isoform 5 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_011131 | 205-555 | in isoform 3 | |||
Sequence: ESCESSSHSIDEVSLSAFGEWTEIPGAHHIIPSGFMRVVELLAEGIPPHVIQLGKPVRCIHWDQASAHPRGPEIEPRGEGDHNHDTGEGGQSGENPQQGRWDEDEPWPVVVECEDCEVIPADHVIVTVSLGVLKRQYTSFFRPCLPTEKVAAIHRLGIGTTDKIFLEFEEPFWGPECNSLQFVWEDEAESCTLTYPPELWYRKICGFDVLYPPERYGHVLSGWICGEEALVMERCDDEAVAEICTEMLRQFTGNPNIPKPRRILRSAWGSNPYFRGSYSYTQVGSSGADVEKLAKPLPYTESSKTAPMQVLFSGEATHRKYYSTTHGALLSGQREAARLIEMYRDLFQQGP → SLLWSIDARVKKMNSGGVSVQSALLLRALVPCMA | ||||||
Alternative sequence | VSP_011132 | 210-555 | in isoform 4 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_011133 | 244-255 | in isoform 9 | |||
Sequence: Missing | ||||||
Compositional bias | 272-292 | Basic and acidic residues | ||||
Sequence: HPRGPEIEPRGEGDHNHDTGE | ||||||
Alternative sequence | VSP_011134 | 282-417 | in isoform 8 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_011135 | 457-512 | in isoform 7 | |||
Sequence: GNPNIPKPRRILRSAWGSNPYFRGSYSYTQVGSSGADVEKLAKPLPYTESSKTAPM → AHAGALLRGGHTP | ||||||
Alternative sequence | VSP_011136 | 458-512 | in isoform 6 | |||
Sequence: NPNIPKPRRILRSAWGSNPYFRGSYSYTQVGSSGADVEKLAKPLPYTESSKTAPM → GLKWGGCGEASQAPALHRELQDSAHAGALLRGGHTP | ||||||
Alternative sequence | VSP_011137 | 510 | in isoform 2 | |||
Sequence: A → AHRSSTEQQPGHLLPSKCPEQSLDPSRGSIK | ||||||
Alternative sequence | VSP_011138 | 516-555 | in isoform 6 and isoform 7 | |||
Sequence: FSGEATHRKYYSTTHGALLSGQREAARLIEMYRDLFQQGP → LHHPRCSALWPARGRPAHRDVPRPLPAGALKGVLTAKCVP |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ567473 EMBL· GenBank· DDBJ | CAD98866.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ567474 EMBL· GenBank· DDBJ | CAD98867.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ567475 EMBL· GenBank· DDBJ | CAD98868.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ567476 EMBL· GenBank· DDBJ | CAD98869.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ567477 EMBL· GenBank· DDBJ | CAD98870.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ567478 EMBL· GenBank· DDBJ | CAD98871.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ567479 EMBL· GenBank· DDBJ | CAD98872.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ567480 EMBL· GenBank· DDBJ | CAD98873.1 EMBL· GenBank· DDBJ | mRNA | ||
AF495851 EMBL· GenBank· DDBJ | AAN32908.1 EMBL· GenBank· DDBJ | mRNA | ||
AF495852 EMBL· GenBank· DDBJ | AAN32909.1 EMBL· GenBank· DDBJ | mRNA | ||
AF495853 EMBL· GenBank· DDBJ | AAN32910.1 EMBL· GenBank· DDBJ | mRNA | ||
AF498364 EMBL· GenBank· DDBJ | AAN32915.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL831731 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL831781 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC004831 EMBL· GenBank· DDBJ | AAH04831.1 EMBL· GenBank· DDBJ | mRNA |