Q99K23 · UFSP2_MOUSE
- ProteinUfm1-specific protease 2
- GeneUfsp2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids461 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Thiol-dependent isopeptidase that specifically cleaves UFM1, a ubiquitin-like modifier protein, from conjugated proteins, such as CD274/PD-L1, CYB5R3, DDRGK1, MRE11, RPL26/uL24, TRIP4 and RPL26/uL24 (PubMed:17182609, PubMed:21228277, PubMed:33372156).
While it is also able to mediate the processing of UFM1 precursors, a prerequisite for conjugation reactions, UFSP2 mainly acts as a protein deUFMylase that mediates deconjugation of UFM1 from target proteins (PubMed:17182609, PubMed:21228277).
Mediates deUFMylation of RPL26/uL24, a critical step to release the UFM1 ribosome E3 ligase (UREL) complex during the recycling of 60S ribosome subunits from the endoplasmic reticulum (By similarity).
Catalyzes deUFMylation of TRIP4, regulating intracellular nuclear receptors transactivation and thereby regulate cell proliferation and differentiation (By similarity).
While it is also able to mediate the processing of UFM1 precursors, a prerequisite for conjugation reactions, UFSP2 mainly acts as a protein deUFMylase that mediates deconjugation of UFM1 from target proteins (PubMed:17182609, PubMed:21228277).
Mediates deUFMylation of RPL26/uL24, a critical step to release the UFM1 ribosome E3 ligase (UREL) complex during the recycling of 60S ribosome subunits from the endoplasmic reticulum (By similarity).
Catalyzes deUFMylation of TRIP4, regulating intracellular nuclear receptors transactivation and thereby regulate cell proliferation and differentiation (By similarity).
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 294 | |||||
Sequence: C | ||||||
Active site | 418 | |||||
Sequence: D | ||||||
Active site | 420 | |||||
Sequence: H |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | nucleus | |
Molecular Function | deUFMylase activity | |
Biological Process | proteolysis | |
Biological Process | regulation of intracellular estrogen receptor signaling pathway | |
Biological Process | regulation of type II interferon production | |
Biological Process | ribosome disassembly |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameUfm1-specific protease 2
- EC number
- Short namesUfSP2
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ99K23
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 282 | Loss of enzyme activity. | ||||
Sequence: Y → H | ||||||
Mutagenesis | 294 | Loss of enzyme activity. | ||||
Sequence: C → S | ||||||
Mutagenesis | 398 | No effect on enzyme activity. | ||||
Sequence: H → A | ||||||
Mutagenesis | 418 | Reduced enzyme activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 420 | Loss of enzyme activity. | ||||
Sequence: H → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 19 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000280363 | 1-461 | Ufm1-specific protease 2 | |||
Sequence: MDILFRIRGGFDLAFQLAPPKEMFIKNALRQVLSDLTTKLSSDALVLRVCNSSVYLWPNSDANTGELTDSSACKNVVRFIQFDQEEDTKRKFIKKKDKKLTDTQQIVNIDLMLEISTPLGAVTPILERENEEHHYINMSLPIDAVVSVAPEESWGKVRKLLVDAILRQLVDVEKCILRYMKGTSIVVPEPLHFQLPGKKNLVTVLYPSGIPDDQLQAYRKELHDLFNLPHDRPYFKRINAYHFPDELYKDGYIRNPHTYLSPPNIEGSMICVVQGTYAYHHYMQDRIDDNGWGCAYRSLQTICSWFRHQGYTERSIPTHREIQQALVDAGDKPATFVGSRQWIGSIEVQMVLNQLIGVTSKILFVNQGSEMASQGRELANHFQNVGTPVMVGGGVLAHTILGVAWNETTGQIKFLILDPHYTGAEDLQVMLEKGWCGWKSPDFWNKDAYYNLCLPQRPNAL |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed at high level in brain, kidney, stomach, skeletal muscle, liver, pancreas, spleen and testis.
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length461
- Mass (Da)52,515
- Last updated2001-06-01 v1
- ChecksumA04A842782C2A0CE
Computationally mapped potential isoform sequences
There are 5 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1B0GQY6 | A0A1B0GQY6_MOUSE | Ufsp2 | 44 | ||
A0A1B0GSY4 | A0A1B0GSY4_MOUSE | Ufsp2 | 81 | ||
A0A1B0GSK1 | A0A1B0GSK1_MOUSE | Ufsp2 | 117 | ||
A0A1B0GSM1 | A0A1B0GSM1_MOUSE | Ufsp2 | 211 | ||
A0A1B0GRT2 | A0A1B0GRT2_MOUSE | Ufsp2 | 91 |
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK075795 EMBL· GenBank· DDBJ | BAC35964.1 EMBL· GenBank· DDBJ | mRNA | ||
AK078309 EMBL· GenBank· DDBJ | BAC37214.1 EMBL· GenBank· DDBJ | mRNA | Sequence problems. | |
BC005503 EMBL· GenBank· DDBJ | AAH05503.1 EMBL· GenBank· DDBJ | mRNA |