Q99JB8 · PACN3_MOUSE

  • Protein
    Protein kinase C and casein kinase II substrate protein 3
  • Gene
    Pacsin3
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentendosome
Cellular Componentplasma membrane
Molecular Functioncalcium channel inhibitor activity
Molecular Functioncytoskeletal protein binding
Molecular Functionidentical protein binding
Molecular Functionlipid binding
Molecular Functionphospholipid binding
Biological Processcytoskeleton organization
Biological Processendocytosis
Biological Processnegative regulation of calcium ion transport
Biological Processnegative regulation of endocytosis
Biological Processplasma membrane tubulation
Biological Processpositive regulation of membrane protein ectodomain proteolysis
Biological Processregulation of endocytosis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Protein kinase C and casein kinase II substrate protein 3

Gene names

    • Name
      Pacsin3

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q99JB8
  • Secondary accessions
    • Q9EQP9

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Cell membrane
; Peripheral membrane protein
Note: Detected at the inner aspect of the plasma membrane in myotubes.

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis415Loss of DNM1-, SYNJ1- and WASL-binding. Loss of effect on transferrin endocytosis.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 30 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00001618011-424Protein kinase C and casein kinase II substrate protein 3
Modified residue276Phosphoserine
Modified residue303Phosphoserine
Modified residue319Phosphoserine
Modified residue324Phosphothreonine
Modified residue327Phosphoserine
Modified residue354Phosphoserine
Modified residue383Phosphoserine

Post-translational modification

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Highly expressed in skeletal muscle, heart and lung; also detected in brain, kidney and uterus (at protein level).

Gene expression databases

Interaction

Subunit

Homodimer. May form heterooligomers with other PACSINs. Interacts (via SH3 domain) with DNM1, SYNJ1 and WASL. Interacts with TRPV4.

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for domain, coiled coil, compositional bias, region.

TypeIDPosition(s)Description
Domain10-280F-BAR
Coiled coil23-272
Compositional bias154-172Basic and acidic residues
Region154-184Disordered
Region312-362Disordered
Domain363-424SH3

Domain

The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation.

Sequence similarities

Belongs to the PACSIN family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    424
  • Mass (Da)
    48,585
  • Last updated
    2001-06-01 v1
  • Checksum
    00475BC0321485B7
MAPEEDAGGEVLGGSFWEAGNYRRTVQRVEDGHRLCGDLVSCFQERARIEKAYAQQLADWARKWRGAVEKGPQYGTLEKAWHAFFTAAERLSELHLEVREKLHGPDSERVRTWQRGAFHRPVLGGFRESRAAEDGFRKAQKPWLKRLKEVEASKKSYHTARKDEKTAQTRESHAKADSSMSQEQLRKLQERVGRCTKEAEKMKTQYEQTLAELNRYTPRYMEDMEQAFESCQAAERQRLLFFKDVLLTLHQHLDLSSSDKFHELHRDLQQSIEAASDEEDLRWWRSTHGPGMAMNWPQFEEWSLDTQRAISRKEKGGRSPDEVTLTSIVPTRDGTAPPPQSPSSPGSGQDEDWSDEESPRKVATGVRVRALYDYAGQEADELSFRAGEELLKMSEEDEQGWCQGQLQSGRIGLYPANYVECVGA

Computationally mapped potential isoform sequences

There are 7 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
B1AW91B1AW91_MOUSEPacsin3168
B1AW92B1AW92_MOUSEPacsin395
B1AW93B1AW93_MOUSEPacsin3125
B1AW94B1AW94_MOUSEPacsin3114
B1AW95B1AW95_MOUSEPacsin364
A6PWR1A6PWR1_MOUSEPacsin3178
F6W8Y8F6W8Y8_MOUSEPacsin3133

Features

Showing features for compositional bias, sequence conflict.

TypeIDPosition(s)Description
Compositional bias154-172Basic and acidic residues
Sequence conflict360in Ref. 1; AAG31022

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF149824
EMBL· GenBank· DDBJ
AAG31022.1
EMBL· GenBank· DDBJ
mRNA
AF242531
EMBL· GenBank· DDBJ
AAK29208.1
EMBL· GenBank· DDBJ
mRNA
BC003884
EMBL· GenBank· DDBJ
AAH03884.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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