Q99J87 · DHX58_MOUSE

  • Protein
    ATP-dependent RNA helicase DHX58
  • Gene
    Dhx58
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Acts as a regulator of RIGI and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to RIGI and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on RIG-I signaling may involve the following mechanisms: competition with RIGI for binding to the viral RNA, binding to RIGI and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by RIGI and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate.

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site24-31ATP (UniProtKB | ChEBI)
Binding site556Zn2+ (UniProtKB | ChEBI)
Binding site559Zn2+ (UniProtKB | ChEBI)
Binding site612Zn2+ (UniProtKB | ChEBI)
Binding site615Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionDNA binding
Molecular Functiondouble-stranded RNA binding
Molecular FunctionRNA helicase activity
Molecular Functionsingle-stranded RNA binding
Molecular Functionzinc ion binding
Biological Processantiviral innate immune response
Biological Processcytoplasmic pattern recognition receptor signaling pathway
Biological Processnegative regulation of innate immune response
Biological Processnegative regulation of MDA-5 signaling pathway
Biological Processnegative regulation of RIG-I signaling pathway
Biological Processnegative regulation of type I interferon production
Biological Processpositive regulation of MDA-5 signaling pathway
Biological Processpositive regulation of RIG-I signaling pathway
Biological Processpositive regulation of type I interferon production
Biological Processresponse to bacterium
Biological Processresponse to virus

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-dependent RNA helicase DHX58
  • EC number
  • Alternative names
    • ATP-dependent helicase LGP2
    • Protein D11Lgp2
    • RIG-I-like receptor 3 (RLR-3)
    • RIG-I-like receptor Lgp2 (RLR)

Gene names

    • Name
      Dhx58
    • Synonyms
      D11lgp2e, Lgp2

Organism names

  • Taxonomic identifier
  • Strains
    • C57BL/6J
    • Czech II
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q99J87
  • Secondary accessions
    • A2A5E9
    • Q9D1X4

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Embryonic lethality at a high frequency. Adult female that survive show an enlarged uterus filled with fluid resulting from vaginal atresia.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis30Abolishes IFNB1 production upon infection with various viruses.

Variants

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The viewer provides 51 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001020111-678ATP-dependent RNA helicase DHX58

Proteomic databases

PTM databases

Expression

Tissue specificity

Highly expressed in mammary tissues. Expressed in liver and testis. Expressed at lower level in spleen, embryo, mammary gland and breast tumors.

Induction

By interferon (IFN), virus infection, or intracellular dsRNA.

Gene expression databases

Interaction

Subunit

Monomer in the absence of dsRNA. Homodimer in the presence of dsRNA. Interacts with RIGI (via CARD domain), MAVS/IPS1 and DDX60. Found in a complex with RIGI and IFIH1/MDA5. Interacts with ANKRD17. Directly interacts with ATG5 and ATG12, either as ATG5 and ATG12 monomers or as ATG12-ATG5 conjugates (By similarity).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for domain, motif, coiled coil, region.

TypeIDPosition(s)Description
Domain11-188Helicase ATP-binding
Motif131-134DECH box
Domain353-514Helicase C-terminal
Coiled coil489-546
Domain542-669RLR CTR
Region572-655RNA-binding

Domain

The RLR CTR domain is capable of inhibiting dimerization and signaling of RIGI and also facilitates binding of dsRNA.

Sequence similarities

Belongs to the helicase family. RLR subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    678
  • Mass (Da)
    76,709
  • Last updated
    2011-07-27 v2
  • Checksum
    D907A30E3AD376A8
MELRPYQWEVILPALEGKNIIIWLPTGAGKTRAAAFVAKRHLETVDRGKVVVLVNRVHLVSQHAEEFRRMLDKHWTVTTLSGDMGSRAGFGLMARSHDLLICTAELLQLALNSSEEDEHVELREFSLIVVDECHHTHKDTVYNTILSRYLEQKLKKAEPLPQVLGLTASPGTGGATKLQGAIDHILQLCANLDTCHIMSPKNCYSQLLMHNPKPCKQYDLCQRRAQDPFGDLIKKLMNQIHQQLEMPDLKQQFGTQMYEQQVVQLCKDAAEAGLQEQRVYALHLRRYNDALFIHDTVRARDALDMLQDFYDRERTTKTQMVRAESWLLKLFDDHKNVLGQLAARGPENPKLEMLERILLKQFGSPGHTRGIIFTRTRQTASSLLLWLRQQPCLQTVGIKPQMLIGAGNTSQSTHMTQKDQQEVIQEFRDGILSLLVATSVAEEGLDIAQCNVVVRYGLLTNEISMVQARGRARAGQSVYSFLATEGSREMKRELTNEALEVLMEKAVAAVQKMDPDEFKAKIRDLQQASLVKRAARAAHREIQQGQFLPEHVQLLCINCMVAVGYGSDLRKVEGTHHVNVNPNFSVYYTTSQNPVVINKVFKDWRPGGTIRCSNCGEVWGFQMIYKSVTLPVLKIGSILLETPRGKIQAKKWSRVPFSIPVFDILQDCTQSLSELSLD

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict638in Ref. 1; AAK15474/AAK15475 and 3; AAH29209

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF316999
EMBL· GenBank· DDBJ
AAK15474.1
EMBL· GenBank· DDBJ
mRNA
AF317000
EMBL· GenBank· DDBJ
AAK15475.1
EMBL· GenBank· DDBJ
Genomic DNA
AL591469
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC029209
EMBL· GenBank· DDBJ
AAH29209.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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