Q99244 · CAC1D_MESAU
- ProteinVoltage-dependent L-type calcium channel subunit alpha-1D
- GeneCACNA1D
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1610 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, and by benzothiazepines.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | voltage-gated calcium channel complex | |
Molecular Function | metal ion binding | |
Molecular Function | voltage-gated calcium channel activity | |
Biological Process | positive regulation of adenylate cyclase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameVoltage-dependent L-type calcium channel subunit alpha-1D
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Cricetidae > Cricetinae > Mesocricetus
Accessions
- Primary accessionQ99244
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-125 | Cytoplasmic | ||||
Sequence: MMMMMMKKMQHQRQQQEDHANEANYARGTRPPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWK | ||||||
Transmembrane | 126-144 | Helical; Name=S1 of repeat I | ||||
Sequence: PFDIFILLAIFANCVALAI | ||||||
Topological domain | 145-162 | Extracellular | ||||
Sequence: YIPFPEDDSNSTNHNLEK | ||||||
Transmembrane | 163-182 | Helical; Name=S2 of repeat I | ||||
Sequence: VEYAFLIIFTVETFLKIIAY | ||||||
Topological domain | 183-194 | Cytoplasmic | ||||
Sequence: GLLLHPNAYVRN | ||||||
Transmembrane | 195-213 | Helical; Name=S3 of repeat I | ||||
Sequence: GWNLLDFVIVIVGLFSVIL | ||||||
Topological domain | 214-234 | Extracellular | ||||
Sequence: EQLTKETEGGNHSSGKSGGFD | ||||||
Transmembrane | 235-253 | Helical; Name=S4 of repeat I | ||||
Sequence: VKALRAFRVLRPLRLVSGV | ||||||
Topological domain | 254-272 | Cytoplasmic | ||||
Sequence: PSLQVVLNSIIKAMVPLLH | ||||||
Transmembrane | 273-292 | Helical; Name=S5 of repeat I | ||||
Sequence: IALLVLFVIIIYAIIGLELF | ||||||
Topological domain | 293-380 | Extracellular | ||||
Sequence: IGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAVNGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFEL | ||||||
Transmembrane | 381-405 | Helical; Name=S6 of repeat I | ||||
Sequence: PWVYFVSLVIFGSFFVLNLVLGVLS | ||||||
Topological domain | 406-522 | Cytoplasmic | ||||
Sequence: GEFSKEREKAKARGDFQKLRENEQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGSCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSV | ||||||
Transmembrane | 523-542 | Helical; Name=S1 of repeat II | ||||
Sequence: TFYWLVIVLVFLNTLTISSE | ||||||
Topological domain | 543-557 | Extracellular | ||||
Sequence: HYNQPDWLTQIQDIA | ||||||
Transmembrane | 558-576 | Helical; Name=S2 of repeat II | ||||
Sequence: NKVLLALFTCEMLVKMYSL | ||||||
Topological domain | 577-584 | Cytoplasmic | ||||
Sequence: GLQAYFVS | ||||||
Transmembrane | 585-603 | Helical; Name=S3 of repeat II | ||||
Sequence: LFNRFDCFVVCGGITETIL | ||||||
Topological domain | 604-613 | Extracellular | ||||
Sequence: VELELMSPLG | ||||||
Transmembrane | 614-632 | Helical; Name=S4 of repeat II | ||||
Sequence: VSVFRCVRLLRIFKVTRHW | ||||||
Topological domain | 633-651 | Cytoplasmic | ||||
Sequence: TSLSNLVASLLNSMKSIAS | ||||||
Transmembrane | 652-672 | Helical; Name=S5 of repeat II | ||||
Sequence: LLLLLFLFIIIFSLLGMQLFG | ||||||
Topological domain | 673-726 | Extracellular | ||||
Sequence: GKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMI | ||||||
Transmembrane | 727-751 | Helical; Name=S6 of repeat II | ||||
Sequence: VCIYFIILFICGNYILLNVFLAIAV | ||||||
Topological domain | 752-884 | Cytoplasmic | ||||
Sequence: DNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEETEDKDPYPPCDVPVGEEEEEEEEEPEVPAGPRPRRISELNMKEKIVPIPEGSAFFILSKTNPIRVGCHKLINHH | ||||||
Transmembrane | 885-903 | Helical; Name=S1 of repeat III | ||||
Sequence: IFTNLILVFIMLSSAALAA | ||||||
Topological domain | 904-919 | Extracellular | ||||
Sequence: EDPIRSHSFRNTILGY | ||||||
Transmembrane | 920-939 | Helical; Name=S2 of repeat III | ||||
Sequence: FDYAFTAIFTVEILLKMTTF | ||||||
Topological domain | 940-951 | Cytoplasmic | ||||
Sequence: GAFLHKGAFCRN | ||||||
Transmembrane | 952-970 | Helical; Name=S3 of repeat III | ||||
Sequence: YFNLLDMLVVGVSLVSFGI | ||||||
Topological domain | 971-976 | Extracellular | ||||
Sequence: QSSAIS | ||||||
Transmembrane | 977-996 | Helical; Name=S4 of repeat III | ||||
Sequence: VVKILRVLRVLRPLRAINRA | ||||||
Topological domain | 997-1015 | Cytoplasmic | ||||
Sequence: KGLKHVVQCVFVAIRTIGN | ||||||
Transmembrane | 1016-1035 | Helical; Name=S5 of repeat III | ||||
Sequence: IMIVTTLLQFMFACIGVQLF | ||||||
Topological domain | 1036-1125 | Extracellular | ||||
Sequence: KGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENAGPVYNHRVE | ||||||
Transmembrane | 1126-1146 | Helical; Name=S6 of repeat III | ||||
Sequence: ISIFFIIYIIIVAFFMMNIFV | ||||||
Topological domain | 1147-1203 | Cytoplasmic | ||||
Sequence: GFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSS | ||||||
Transmembrane | 1204-1222 | Helical; Name=S1 of repeat IV | ||||
Sequence: PFEYMMFVLIMLNTLCLAM | ||||||
Topological domain | 1223-1237 | Extracellular | ||||
Sequence: QHYEQSKMFNDAMDI | ||||||
Transmembrane | 1238-1257 | Helical; Name=S2 of repeat IV | ||||
Sequence: LNMVFTGVFTVEMVLKVIAF | ||||||
Topological domain | 1258-1264 | Cytoplasmic | ||||
Sequence: KPKGYFS | ||||||
Transmembrane | 1265-1286 | Helical; Name=S3 of repeat IV | ||||
Sequence: DAWNTFDSLIVIGSIIDVALSE | ||||||
Topological domain | 1287-1311 | Extracellular | ||||
Sequence: ADPTESESLPLPTATPGNSEESNRI | ||||||
Transmembrane | 1312-1331 | Helical; Name=S4 of repeat IV | ||||
Sequence: SITFFRLFRVMRLVKLLSRG | ||||||
Topological domain | 1332-1350 | Cytoplasmic | ||||
Sequence: EGIRTLLWTFIKSFQALPY | ||||||
Transmembrane | 1351-1370 | Helical; Name=S5 of repeat IV | ||||
Sequence: VALLIAMLFFIYAVIGMQMF | ||||||
Topological domain | 1371-1437 | Extracellular | ||||
Sequence: GKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNF | ||||||
Transmembrane | 1438-1462 | Helical; Name=S6 of repeat IV | ||||
Sequence: AIVYFISFYMLCAFLIINLFVAVIM | ||||||
Topological domain | 1463-1610 | Cytoplasmic | ||||
Sequence: DNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGGQCGLCFLSPSRSRS |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000053934 | 1-1610 | Voltage-dependent L-type calcium channel subunit alpha-1D | |||
Sequence: MMMMMMKKMQHQRQQQEDHANEANYARGTRPPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAVNGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLRENEQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGSCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEETEDKDPYPPCDVPVGEEEEEEEEEPEVPAGPRPRRISELNMKEKIVPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENAGPVYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPTESESLPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGGQCGLCFLSPSRSRS | ||||||
Glycosylation | 154 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 224 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 328 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
PTM databases
Expression
Tissue specificity
Expressed in brain, heart and skeletal muscle.
Interaction
Subunit
Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with RIMBP2. Interacts with CABP1 and CABP4, resulting in a near elimination of calcium-dependent inactivation of the channel (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-99 | Disordered | ||||
Sequence: MMMMMMKKMQHQRQQQEDHANEANYARGTRPPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNS | ||||||
Compositional bias | 34-54 | Polar residues | ||||
Sequence: SGEGPTSQPNSSKQTVLSWQA | ||||||
Compositional bias | 63-99 | Polar residues | ||||
Sequence: KAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNS | ||||||
Repeat | 112-408 | I | ||||
Sequence: NPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCAVNGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEF | ||||||
Region | 428-445 | Binding to the beta subunit | ||||
Sequence: EQLEEDLKGYLDWITQAE | ||||||
Region | 448-487 | Disordered | ||||
Sequence: DPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGS | ||||||
Compositional bias | 461-487 | Polar residues | ||||
Sequence: RNTSMPTSETESVNTENVSGEGETQGS | ||||||
Repeat | 508-754 | II | ||||
Sequence: NRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNL | ||||||
Compositional bias | 765-787 | Basic and acidic residues | ||||
Sequence: KEEAEEKERKKIARKESLENKKN | ||||||
Region | 765-846 | Disordered | ||||
Sequence: KEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEETEDKDPYPPCDVPVGEEEEEEEEEPEVPAGPRPRRI | ||||||
Compositional bias | 788-802 | Polar residues | ||||
Sequence: NKPEVNQIANSDNKV | ||||||
Compositional bias | 803-821 | Basic and acidic residues | ||||
Sequence: TIDDYQEETEDKDPYPPCD | ||||||
Compositional bias | 822-836 | Acidic residues | ||||
Sequence: VPVGEEEEEEEEEPE | ||||||
Repeat | 871-1153 | III | ||||
Sequence: NPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENAGPVYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTF | ||||||
Region | 1073-1163 | Dihydropyridine binding | ||||
Sequence: RIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENAGPVYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKN | ||||||
Repeat | 1190-1465 | IV | ||||
Sequence: NPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPTESESLPLPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNF | ||||||
Region | 1418-1484 | Dihydropyridine binding | ||||
Sequence: LCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFK | ||||||
Region | 1430-1473 | Phenylalkylamine binding | ||||
Sequence: EYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWS |
Domain
Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
Sequence similarities
Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1D subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing. Additional isoforms seem to exist.
Q99244-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameHCA3A
- Length1,610
- Mass (Da)182,328
- Last updated1999-07-15 v2
- ChecksumB3B2E3794D936F79
Q99244-3
- NameCACH3B
Q99244-2
- NameCACH3D
- Differences from canonical
- 1261-1303: GYFSDAWNTFDSLIVIGSIIDVALSEADPTESESLPLPTATPG → HYFTDAWNTFDALIVVGSVVDIAITEVN
Features
Showing features for compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 34-54 | Polar residues | ||||
Sequence: SGEGPTSQPNSSKQTVLSWQA | ||||||
Compositional bias | 63-99 | Polar residues | ||||
Sequence: KAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNS | ||||||
Compositional bias | 461-487 | Polar residues | ||||
Sequence: RNTSMPTSETESVNTENVSGEGETQGS | ||||||
Compositional bias | 765-787 | Basic and acidic residues | ||||
Sequence: KEEAEEKERKKIARKESLENKKN | ||||||
Compositional bias | 788-802 | Polar residues | ||||
Sequence: NKPEVNQIANSDNKV | ||||||
Compositional bias | 803-821 | Basic and acidic residues | ||||
Sequence: TIDDYQEETEDKDPYPPCD | ||||||
Compositional bias | 822-836 | Acidic residues | ||||
Sequence: VPVGEEEEEEEEEPE | ||||||
Alternative sequence | VSP_000915 | 1261-1303 | in isoform CACH3D | |||
Sequence: GYFSDAWNTFDSLIVIGSIIDVALSEADPTESESLPLPTATPG → HYFTDAWNTFDALIVVGSVVDIAITEVN |
Keywords
- Coding sequence diversity
- Technical term