Q97Z83 · PRIS_SACS2

Function

function

Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair. Possesses a template-independent 3'-terminal nucleotidyl transferase activity.

Miscellaneous

The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA (PubMed:16273105).

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site, binding site.

133050100150200250300
TypeIDPosition(s)Description
Active site101
Active site103
Binding site116Zn2+ (UniProtKB | ChEBI)
Binding site119Zn2+ (UniProtKB | ChEBI)
Binding site128Zn2+ (UniProtKB | ChEBI)
Binding site131Zn2+ (UniProtKB | ChEBI)
Active site235

GO annotations

AspectTerm
Cellular ComponentDNA-directed RNA polymerase complex
Cellular Componentprimosome complex
Molecular FunctionDNA primase activity
Molecular Functionmetal ion binding
Biological ProcessDNA replication, synthesis of primer

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA primase small subunit PriS
  • EC number

Gene names

    • Name
      priS
    • Synonyms
      priA
    • Ordered locus names
      SSO1048

Organism names

Accessions

  • Primary accession
    Q97Z83

Proteomes

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis164Strong decrease in interaction with PriL.
Mutagenesis165Partial destabilization of PriS-PriL complex.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000467541-330DNA primase small subunit PriS

Proteomic databases

Interaction

Subunit

Heterodimer of a small subunit (PriS) and a large subunit (PriL).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q97Z83priL Q9UWW15EBI-8081502, EBI-8081454

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the eukaryotic-type primase small subunit family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    330
  • Mass (Da)
    37,598
  • Last updated
    2001-10-01 v1
  • Checksum
    E9B3C556E619B66E
MGTFTLHQGQTNLIKSFFRNYYLNAELELPKDMELREFALQPFGSDTYVRHLSFSSSEELRDYLVNRNLPLHLFYSSARYQLPSARNMEEKAWMGSDLLFDIDADHLCKLRSIRFCPVCGNAVVSEKCERDNVETLEYVEMTSECIKRGLEQTRNLVEILEDDFGLKPKVYFSGNRGFHVQVDCYGNCALLDSDERKEIAEYVMGIGVPGYPGGSENAPGWVGRKNRGINGVTIDEQVTIDVKRLIRIPNSLHGKSGLIVKRVPNLDDFEFNETLSPFTGYTIFLPYITIETEVLGSIIKLNRGIPIKIKSSIGIYLHLRNLGEVKAYVR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE006641
EMBL· GenBank· DDBJ
AAK41310.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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