Q97Z83 · PRIS_SACS2
- ProteinDNA primase small subunit PriS
- GenepriS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids330 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair. Possesses a template-independent 3'-terminal nucleotidyl transferase activity.
Miscellaneous
The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA (PubMed:16273105).
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 101 | |||||
Sequence: D | ||||||
Active site | 103 | |||||
Sequence: D | ||||||
Binding site | 116 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 119 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 128 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 131 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 235 | |||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | DNA-directed RNA polymerase complex | |
Cellular Component | primosome complex | |
Molecular Function | DNA primase activity | |
Molecular Function | metal ion binding | |
Biological Process | DNA replication, synthesis of primer |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA primase small subunit PriS
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Sulfolobales > Sulfolobaceae > Saccharolobus
Accessions
- Primary accessionQ97Z83
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 164 | Strong decrease in interaction with PriL. | ||||
Sequence: F → E | ||||||
Mutagenesis | 165 | Partial destabilization of PriS-PriL complex. | ||||
Sequence: G → I |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000046754 | 1-330 | DNA primase small subunit PriS | |||
Sequence: MGTFTLHQGQTNLIKSFFRNYYLNAELELPKDMELREFALQPFGSDTYVRHLSFSSSEELRDYLVNRNLPLHLFYSSARYQLPSARNMEEKAWMGSDLLFDIDADHLCKLRSIRFCPVCGNAVVSEKCERDNVETLEYVEMTSECIKRGLEQTRNLVEILEDDFGLKPKVYFSGNRGFHVQVDCYGNCALLDSDERKEIAEYVMGIGVPGYPGGSENAPGWVGRKNRGINGVTIDEQVTIDVKRLIRIPNSLHGKSGLIVKRVPNLDDFEFNETLSPFTGYTIFLPYITIETEVLGSIIKLNRGIPIKIKSSIGIYLHLRNLGEVKAYVR |
Proteomic databases
Interaction
Subunit
Heterodimer of a small subunit (PriS) and a large subunit (PriL).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q97Z83 | priL Q9UWW1 | 5 | EBI-8081502, EBI-8081454 |
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length330
- Mass (Da)37,598
- Last updated2001-10-01 v1
- ChecksumE9B3C556E619B66E
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE006641 EMBL· GenBank· DDBJ | AAK41310.1 EMBL· GenBank· DDBJ | Genomic DNA |