Q97YX6 · HJE_SACS2
- ProteinCrossover junction endodeoxyribonuclease Hje
- Genehje
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids135 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Acts only on 4-way DNA junctions in a sequence non-specific manner; introduces paired nicks in opposing strands 2 bases 3' of the point of strand exchange only on continuous strands of 4-way junction DNA. Cleaves both mobile and immobile junctions.
Miscellaneous
A second Holliday junction resolving enzyme, Hjc, with different substrate specificity exists in this organism.
Catalytic activity
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
73 nM | 4-way junction DNA at 5 mM MgCl2 | |||||
66 nM | 4-way junction DNA at 15 mM MgCl2 |
kcat is 4.8 min-1 and 10 min-1 at 5 mM and 15 mM MgCl2 respectively.
Temperature Dependence
Optimum temperature is 60 degrees Celsius. Could be higher with a more thermally stable substrate.
Features
Showing features for binding site, site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA binding | |
Molecular Function | endonuclease activity | |
Molecular Function | metal ion binding | |
Biological Process | DNA recombination | |
Biological Process | DNA repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCrossover junction endodeoxyribonuclease Hje
- EC number
- Short namesHje
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Sulfolobales > Sulfolobaceae > Saccharolobus
Accessions
- Primary accessionQ97YX6
Proteomes
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 30 | 100- to 1000-fold reduction in kcat. | ||||
Sequence: S → A, C, or T |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000429159 | 1-135 | Crossover junction endodeoxyribonuclease Hje | |||
Sequence: MNRDIGKNAERELVSILRGEGFNAVRIPTSNSSPNPLPDIFATKGNTLLSIECKSTWENKVKVKEHQVRKLLDFLSMFTMKGVPLIAIKFKQVHEWRVLVPEKAEDIIVTIDNSIPIEDLFKILEKRIEEKILTP |
Proteomic databases
Interaction
Structure
Family & Domains
Sequence similarities
Belongs to the Holliday junction resolvase Hjc family. Hje subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length135
- Mass (Da)15,452
- Last updated2001-10-01 v1
- Checksum13036E9997223BC2
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE006641 EMBL· GenBank· DDBJ | AAK41424.1 EMBL· GenBank· DDBJ | Genomic DNA |