Q97WH0 · RAD50_SACS2
- ProteinDNA double-strand break repair Rad50 ATPase
- Generad50
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids864 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.
Cofactor
Note: Binds 1 zinc ion per homodimer.
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | zinc ion binding | |
Biological Process | double-strand break repair |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameDNA double-strand break repair Rad50 ATPase
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Sulfolobales > Sulfolobaceae > Saccharolobus
Accessions
- Primary accessionQ97WH0
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000138665 | 1-864 | DNA double-strand break repair Rad50 ATPase | |||
Sequence: MRIDKITLTNFLSHEHSEIQFMGEINVIVGQNGAGKSSIIDGIVFSLFRTHSRGNNDNLIRKGSNRGSVTLYLSNEKDKIEIIRDIRSTTEDRLIRNQFPIARSATVVSNEIEKILGIDKDIALSTIIVRQGELDKILENFQEIMGKILKLELIEKLIDSRGPIVEFRKNLENKLRELDRIEQDYNNFKKTVEEKRARVLELKKDKEKLEDEIKNLEKRIKDIKDQFDEYEKKRNQYLKLTTTLKIKEGELNELNRSIEELRKQTENMDQLEKEINELENLRNIKLKFEKYEVLAKSHTEMSANVINLEKEIEEYEKAIRRKEELEPKYLKYKELERKLEELQPKYQQYLKLKSDLDSKLNLKERLEKDASELSNDIDKVNSLEQKVEETRKKQLNLRAQLAKVESLISEKNEIINNISQVEGETCPVCGRPLDEEHKQKIIKEAKSYILQLELNKNELEEELKKITNELNKIEREYRRLSNNKASYDNVMRQLKKLNEEIENLHSEIESLKNIDEEIKKINEEVKELKLYYEEFMRLSKYTKEELDKKRVKLDEMKKKKEEIEKEMRGLESELKGLDRKALESKILDLENKRVKLDEMKKKKGILEDYIRQVKLLQEEVKNLREEVNIIQFDENRYNELKTSLDAYNLSLKEKENRKSRIEGELESLEKDIEEISNRIANYELQLKDREKIINAINKLEKIRSALGERKLQSYIIMTTKQLIENNLNDIISKFDLSIKNVEMEIMPKTGRGRSSSGDILVYTNSGDTLPIVSLSGGERIALSIALRLAIAKALMSNTNFFILDEPTIHLDDQRKAYLIEIIRAAKESVPQIIVVTHDEEVVQAADYVIRVEKRGNKSFVREET |
Proteomic databases
Interaction
Subunit
Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for coiled coil, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Coiled coil | 176-319 | |||||
Sequence: RELDRIEQDYNNFKKTVEEKRARVLELKKDKEKLEDEIKNLEKRIKDIKDQFDEYEKKRNQYLKLTTTLKIKEGELNELNRSIEELRKQTENMDQLEKEINELENLRNIKLKFEKYEVLAKSHTEMSANVINLEKEIEEYEKAI | ||||||
Coiled coil | 376-413 | |||||
Sequence: DIDKVNSLEQKVEETRKKQLNLRAQLAKVESLISEKNE | ||||||
Domain | 380-478 | Zinc-hook | ||||
Sequence: VNSLEQKVEETRKKQLNLRAQLAKVESLISEKNEIINNISQVEGETCPVCGRPLDEEHKQKIIKEAKSYILQLELNKNELEEELKKITNELNKIEREYR | ||||||
Coiled coil | 440-697 | |||||
Sequence: KIIKEAKSYILQLELNKNELEEELKKITNELNKIEREYRRLSNNKASYDNVMRQLKKLNEEIENLHSEIESLKNIDEEIKKINEEVKELKLYYEEFMRLSKYTKEELDKKRVKLDEMKKKKEEIEKEMRGLESELKGLDRKALESKILDLENKRVKLDEMKKKKGILEDYIRQVKLLQEEVKNLREEVNIIQFDENRYNELKTSLDAYNLSLKEKENRKSRIEGELESLEKDIEEISNRIANYELQLKDREKIINAIN |
Domain
The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer.
Sequence similarities
Belongs to the SMC family. RAD50 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length864
- Mass (Da)101,602
- Last updated2001-09-26 v1
- Checksum657076AEA9B709FC
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE006641 EMBL· GenBank· DDBJ | AAK42417.1 EMBL· GenBank· DDBJ | Genomic DNA |