Q97UY8 · GLCV_SACS2
- ProteinGlucose import ATP-binding protein GlcV
- GeneglcV
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids353 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Part of the ABC transporter complex GlcSTUV involved in glucose uptake (Probable). Responsible for energy coupling to the transport system (PubMed:12823973, PubMed:14607117).
In vitro, as a free subunit, exhibits a constitutive ATPase activity (PubMed:11807278).
In vitro, as a free subunit, exhibits a constitutive ATPase activity (PubMed:11807278).
Catalytic activity
- ATP + D-glucose(out) + H2O = ADP + D-glucose(in) + H+ + phosphateThis reaction proceeds in the forward direction.
CHEBI:30616 + D-glucose (out)CHEBI:4167+ CHEBI:15377 = CHEBI:456216 + D-glucose (in)CHEBI:4167+ CHEBI:15378 + CHEBI:43474
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
290 μM | ATP |
pH Dependence
Optimum pH is 6.5 for ATPase activity.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing | |
Molecular Function | ABC-type ferric iron transporter activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameGlucose import ATP-binding protein GlcV
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Sulfolobales > Sulfolobaceae > Saccharolobus
Accessions
- Primary accessionQ97UY8
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 142 | Decrease in ATPase activity. Can form dimers. | ||||
Sequence: S → A | ||||||
Mutagenesis | 144 | Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166. | ||||
Sequence: G → A | ||||||
Mutagenesis | 166 | Loss of ATPase activity. Can form dimers in the presence of ATP-Mg2+. Forms an active heterodimer; when associated with A-144. | ||||
Sequence: E → A | ||||||
Mutagenesis | 166 | Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg2+. | ||||
Sequence: E → Q |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000447615 | 1-353 | Glucose import ATP-binding protein GlcV | |||
Sequence: MVRIIVKNVSKVFKKGKVVALDNVNINIENGERFGILGPSGAGKTTFMRIIAGLDVPSTGELYFDDRLVASNGKLIVPPEDRKIGMVFQTWALYPNLTAFENIAFPLTNMKMSKEEIRKRVEEVAKILDIHHVLNHFPRELSGGQQQRVALARALVKDPSLLLLDEPFSNLDARMRDSARALVKEVQSRLGVTLLVVSHDPADIFAIADRVGVLVKGKLVQVGKPEDLYDNPVSIQVASLIGEINELEGKVTNEGVVIGSLRFPVSVSSDRAIIGIRPEDVKLSKDVIKDDSWILVGKGKVKVIGYQGGLFRITITPLDSEEEIFTYSDHPIHSGEEVLVYVRKDKIKVFEKN |
Proteomic databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 4-241 | ABC transporter | ||||
Sequence: IIVKNVSKVFKKGKVVALDNVNINIENGERFGILGPSGAGKTTFMRIIAGLDVPSTGELYFDDRLVASNGKLIVPPEDRKIGMVFQTWALYPNLTAFENIAFPLTNMKMSKEEIRKRVEEVAKILDIHHVLNHFPRELSGGQQQRVALARALVKDPSLLLLDEPFSNLDARMRDSARALVKEVQSRLGVTLLVVSHDPADIFAIADRVGVLVKGKLVQVGKPEDLYDNPVSIQVASLI |
Domain
Consists of a typical ABC-ATPase domain, comprising two subdomains, connected by a linker region to a C-terminal domain of unknown function.
Sequence similarities
Belongs to the ABC transporter superfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length353
- Mass (Da)39,113
- Last updated2001-10-01 v1
- Checksum772D42F471DCD980
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE006641 EMBL· GenBank· DDBJ | AAK42960.1 EMBL· GenBank· DDBJ | Genomic DNA |