Q97LU2 · FABV_CLOAB

Function

function

Involved in the fatty acid synthesis (FAS II). Catalyzes the reduction of the carbon-carbon double bond of crotonyl-CoA to yield butyryl-CoA.

Miscellaneous

Possesses high activity for the reduction reaction, but no activity for the reverse oxidation reaction.

Catalytic activity

Biotechnology

Used in the biosynthesis of medium-chain volatile alcohols as biofuels engineered by microorganisms. The switch from the native flavin-dependent enoyl-CoA reductase used in the production of n-butanol, a key second-generation biofuel, to a flavin-independent trans-enoyl-CoA reductase from C.acetobutylicum leads to an order of magnitude increase in product yield in engineered E.coli.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
105.4 μMNAD6.225
kcat is 28.2 sec-1 for reductase activity (at pH 6.2 and 25 degrees Celsius).

Pathway

Lipid metabolism; fatty acid biosynthesis.

Features

Showing features for binding site, site, active site.

Type
IDPosition(s)Description
Binding site47-52NAD+ (UniProtKB | ChEBI)
Binding site74-75NAD+ (UniProtKB | ChEBI)
Site75Plays an important role in discriminating NADH against NADPH
Binding site111-112NAD+ (UniProtKB | ChEBI)
Binding site139-140NAD+ (UniProtKB | ChEBI)
Binding site225substrate
Active site235Proton donor
Binding site244NAD+ (UniProtKB | ChEBI)
Binding site274-276NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionenoyl-[acyl-carrier-protein] reductase (NADH) activity
Molecular FunctionNAD binding
Molecular Functiontrans-2-enoyl-CoA reductase (NADH) activity
Biological Processfatty acid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Trans-2-enoyl-CoA reductase [NADH]
  • EC number
  • Short names
    TER

Gene names

    • Name
      fabV
    • Ordered locus names
      CA_C0462

Organism names

Accessions

  • Primary accession
    Q97LU2

Proteomes

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis11Slight decrease of catalytic efficiency and 3-fold increase of the affinity for NADH compared to wild-type.
Mutagenesis75Able to use both NADH and NADPH as cofactor.
Mutagenesis22512-fold decrease of catalytic efficiency and slight decrease of the affinity for NADH compared to wild-type.
Mutagenesis235Loss of reductase activity.
Mutagenesis244Loss of reductase activity.
Mutagenesis245Slight decrease of catalytic efficiency and affinity for NADH compared to wild-type.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002200401-398Trans-2-enoyl-CoA reductase [NADH]

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the TER reductase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    398
  • Mass (Da)
    45,650
  • Last updated
    2001-10-01 v1
  • Checksum
    59324A21CA466DFC
MIVKAKFVKGFIRDVHPYGCRREVLNQIDYCKKAIGFRGPKKVLIVGASSGFGLATRISVAFGGPEAHTIGVSYETGATDRRIGTAGWYNNIFFKEFAKKKGLVAKNFIEDAFSNETKDKVIKYIKDEFGKIDLFVYSLAAPRRKDYKTGNVYTSRIKTILGDFEGPTIDVERDEITLKKVSSASIEEIEETRKVMGGEDWQEWCEELLYEDCFSDKATTIAYSYIGSPRTYKIYREGTIGIAKKDLEDKAKLINEKLNRVIGGRAFVSVNKALVTKASAYIPTFPLYAAILYKVMKEKNIHENCIMQIERMFSEKIYSNEKIQFDDKGRLRMDDLELRKDVQDEVDRIWSNITPENFKELSDYKGYKKEFMNLNGFDLDGVDYSKDLDIELLRKLEP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE001437
EMBL· GenBank· DDBJ
AAK78442.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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