Q96V44 · LAD_HYPJE

Function

function

Catalyzes the NAD-dependent oxidation of L-arabinitol to L-xylulose in the fungal L-arabinose catabolic pathway. L-arabinose catabolism is important for using plant material as a carbon source. Can partially compensate for xylitol dehydrogenase in xdh1 mutants. Also oxidizes galactitol to L-xylo-3-hexulose as an alternative to the standard Leloir pathway for D-galactose metabolism. NADP cannot act as a cosubstrate.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per subunit.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
18 mML-arabinitol7
200 mMxylitol7
4.5 mML-arabinitol8.6
25 mMD-talitol8.6
60 mMgalactitol8.6
46 mMD-sorbitol8.6
11.3 mMD-allitol8.6
37 mML-mannitol8.6
191 mML-iditol8.6
580 mMD-arabino-3-hexulose8.6
81 mML-xylo-3-hexulose8.6
96 mMD-fructose8.6
81 mMD-psicose8.6
19 mML-sorbitol8.6
28 mML-tagatose8.6
115 mMD-sorbose8.6
180 μMNAD7
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
27 nmol/sec/mg9with L-arabinitol as substrate, 10
10 nmol/sec/mg7with L-arabinitol as substrate
6 nmol/sec/mg7with xylitol as substrate
0.213 nmol/sec/mg8.6with L-arabinitol as substrate
0.146 nmol/sec/mg8.6with D-talitol as substrate
0.012 nmol/sec/mg8.6with galactitol as substrate
0.034 nmol/sec/mg8.6with D-sorbitol as substrate
0.008 nmol/sec/mg8.6with D-allitol as substrate
0.027 nmol/sec/mg8.6with L-mannitol as substrate
0.021 nmol/sec/mg8.6with L-iditol as substrate
0.969 nmol/sec/mg8.6with D-arabino-3-hexulose as substrate
0.197 nmol/sec/mg8.6with L-xylo-3-hexulose as substrate
0.008 nmol/sec/mg8.6with D-fructose as substrate
0.011 nmol/sec/mg8.6with D-psicose as substrate
0.001 nmol/sec/mg8.6with L-sorbitol as substrate
0.003 nmol/sec/mg8.6with L-tagatose as substrate
0.001 nmol/sec/mg8.6with D-sorbose as substrate

pH Dependence

Optimum pH is 9.4. Active from pH 7 to pH 11.

Temperature Dependence

Optimum temperature is 55-65 degrees Celsius.

Pathway

Carbohydrate degradation; L-arabinose degradation via L-arabinitol; D-xylulose 5-phosphate from L-arabinose (fungal route): step 2/5.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site66Zn2+ 1 (UniProtKB | ChEBI); catalytic
Binding site91Zn2+ 1 (UniProtKB | ChEBI); catalytic
Binding site92Zn2+ 1 (UniProtKB | ChEBI); catalytic
Binding site121Zn2+ 2 (UniProtKB | ChEBI); structural
Binding site124Zn2+ 2 (UniProtKB | ChEBI); structural
Binding site127Zn2+ 2 (UniProtKB | ChEBI); structural
Binding site135Zn2+ 2 (UniProtKB | ChEBI); structural
Binding site176Zn2+ 1 (UniProtKB | ChEBI); catalytic
Binding site203-204NAD+ (UniProtKB | ChEBI)
Binding site224NAD+ (UniProtKB | ChEBI)
Binding site229NAD+ (UniProtKB | ChEBI)
Binding site296NAD+ (UniProtKB | ChEBI)
Binding site320-322NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionL-arabinitol 4-dehydrogenase activity
Molecular Functionmetal ion binding
Biological Processarabinose catabolic process
Biological ProcessD-xylose catabolic process
Biological Processgalactose catabolic process
Biological ProcessL-arabinose catabolic process to xylulose 5-phosphate

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    L-arabinitol 4-dehydrogenase
  • EC number
  • Short names
    LAD

Gene names

    • Name
      lad1

Organism names

  • Taxonomic identifier
  • Strains
    • ATCC 56765 / Rut C-30
    • ATCC 26921 / CBS 392.92 / QM9414
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Hypocreaceae > Trichoderma

Accessions

  • Primary accession
    Q96V44

Organism-specific databases

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis224-225Alters cofactor specificity from NAD to NADP; when associated with T-362.
Mutagenesis362Alters cofactor specificity from NAD to NADP; when associated with 224-SR-225.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004184031-377L-arabinitol 4-dehydrogenase

Post-translational modification

The N-terminus is blocked.

Expression

Induction

Only expressed when grown on L-arabinose.

Interaction

Subunit

Homotetramer.

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    377
  • Mass (Da)
    39,886
  • Last updated
    2001-12-01 v1
  • Checksum
    5015D4A61D948D1D
MSPSAVDDAPKATGAAISVKPNIGVFTNPKHDLWISEAEPSADAVKSGADLKPGEVTIAVRSTGICGSDVHFWHAGCIGPMIVEGDHILGHESAGEVIAVHPTVSSLQIGDRVAIEPNIICNACEPCLTGRYNGCEKVEFLSTPPVPGLLRRYVNHPAVWCHKIGNMSWENGALLEPLSVALAGMQRAKVQLGDPVLVCGAGPIGLVSMLCAAAAGACPLVITDISESRLAFAKEICPRVTTHRIEIGKSAEETAKSIVSSFGGVEPAVTLECTGVESSIAAAIWASKFGGKVFVIGVGKNEISIPFMRASVREVDIQLQYRYSNTWPRAIRLIESGVIDLSKFVTHRFPLEDAVKAFETSADPKSGAIKVMIQSLD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF355628
EMBL· GenBank· DDBJ
AAL08944.1
EMBL· GenBank· DDBJ
mRNA
AY225444
EMBL· GenBank· DDBJ
AAP57209.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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