Q96SZ5 · AEDO_HUMAN
- Protein2-aminoethanethiol dioxygenase
- GeneADO
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids270 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plays a vital role in regulating thiol metabolism and preserving oxygen homeostasis by oxidizing the sulfur of cysteamine and N-terminal cysteine-containing proteins to their corresponding sulfinic acids using O2 as a cosubstrate (PubMed:17581819, PubMed:29752763, PubMed:31273118, PubMed:32601061).
Catalyzes the oxidation of cysteamine (2-aminoethanethiol) to hypotaurine (PubMed:17581819, PubMed:29752763, PubMed:32601061).
Catalyzes the oxidation of regulators of G-protein signaling 4 (RGS4) and 5 (RGS5) and interleukin-32 (IL32) (PubMed:31273118, PubMed:32601061).
Catalyzes the oxidation of cysteamine (2-aminoethanethiol) to hypotaurine (PubMed:17581819, PubMed:29752763, PubMed:32601061).
Catalyzes the oxidation of regulators of G-protein signaling 4 (RGS4) and 5 (RGS5) and interleukin-32 (IL32) (PubMed:31273118, PubMed:32601061).
Catalytic activity
- cysteamine + O2 = H+ + hypotaurineThis reaction proceeds in the forward direction.
- N-terminal L-cysteinyl-[protein] + O2 = H+ + N-terminal S-hydroxy-S-oxy-L-cysteinyl-[protein]This reaction proceeds in the forward direction.
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
3.4 mM | cysteamine | |||||
123 μM | RGS4 | |||||
71.51 μM | RGS5 | |||||
33.6 μM | IL32 |
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | cysteamine dioxygenase activity | |
Molecular Function | iron ion binding | |
Biological Process | cellular response to hypoxia |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-aminoethanethiol dioxygenase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ96SZ5
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Disease & Variants
Features
Showing features for natural variant, mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_025333 | 25 | in dbSNP:rs2236295 | |||
Sequence: G → W | ||||||
Natural variant | VAR_025334 | 39 | in dbSNP:rs10995311 | |||
Sequence: P → A | ||||||
Mutagenesis | 87 | Moderate reduction in enzyme activity. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 222 | Significant reduction in enzyme activity. | ||||
Sequence: Y → A or F | ||||||
Mutagenesis | 223 | No significant reduction in enzyme activity. | ||||
Sequence: Y → A | ||||||
Natural variant | VAR_033691 | 266 | in dbSNP:rs569705 | |||
Sequence: P → S |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 321 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data), cross-link.
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000089784 | 1-270 | UniProt | 2-aminoethanethiol dioxygenase | |||
Sequence: MPRDNMASLIQRIARQACLTFRGSGGGRGASDRDAASGPEAPMQPGFPENLSKLKSLLTQLRAEDLNIAPRKATLQPLPPNLPPVTYMHIYETDGFSLGVFLLKSGTSIPLHDHPGMHGMLKVLYGTVRISCMDKLDAGGGQRPRALPPEQQFEPPLQPREREAVRPGVLRSRAEYTEASGPCILTPHRDNLHQIDAVEGPAAFLDILAPPYDPDDGRDCHYYRVLEPVRPKEASSSACDLPREVWLLETPQADDFWCEGEPYPGPKVFP | |||||||
Modified residue (large scale data) | 186 | PRIDE | Phosphothreonine | ||||
Sequence: T | |||||||
Cross-link | 220↔223 | UniProt | 3'-(S-cysteinyl)-tyrosine (Cys-Tyr) | ||||
Sequence: CHYY |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Monomer.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q96SZ5 | CAP2 P40123 | 3 | EBI-11102284, EBI-1051165 | |
BINARY | Q96SZ5 | DISC1 Q9NRI5-2 | 3 | EBI-11102284, EBI-11988027 | |
BINARY | Q96SZ5 | FAM76B Q5HYJ3 | 2 | EBI-11102284, EBI-751192 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 21-48 | Disordered | ||||
Sequence: FRGSGGGRGASDRDAASGPEAPMQPGFP | ||||||
Region | 140-164 | Disordered | ||||
Sequence: GGQRPRALPPEQQFEPPLQPREREA |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length270
- Mass (Da)29,751
- Last updated2006-02-21 v2
- Checksum6B496CCF16E92C9D
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0AA34QVG9 | A0AA34QVG9_HUMAN | ADO | 295 |
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL133417 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH471083 EMBL· GenBank· DDBJ | EAW54235.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC018660 EMBL· GenBank· DDBJ | AAH18660.3 EMBL· GenBank· DDBJ | mRNA | ||
BC028589 EMBL· GenBank· DDBJ | AAH28589.2 EMBL· GenBank· DDBJ | mRNA | ||
BC067740 EMBL· GenBank· DDBJ | AAH67740.2 EMBL· GenBank· DDBJ | mRNA | ||
AK027453 EMBL· GenBank· DDBJ | BAB55123.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |