Q96JF0 · SIAT2_HUMAN
- ProteinBeta-galactoside alpha-2,6-sialyltransferase 2
- GeneST6GAL2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids529 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. Has alpha-2,6-sialyltransferase activity toward oligosaccharides that have the Gal-beta-1,4-GlcNAc sequence at the non-reducing end of their carbohydrate groups, but it has weak or no activities toward glycoproteins and glycolipids.
Catalytic activity
- a beta-D-galactoside + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2->6)-beta-D-galactosyl derivative + CMP + H+
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.74 mM | Gal-beta-1,4-GlcNAc-beta-O-octyl | |||||
0.71 mM | Gal-beta-1,4-GlcNAc | |||||
0.48 mM | lacto-N-neotetraose |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Golgi apparatus | |
Cellular Component | Golgi cisterna membrane | |
Cellular Component | Golgi membrane | |
Molecular Function | beta-galactoside alpha-2,6-sialyltransferase activity | |
Biological Process | oligosaccharide metabolic process | |
Biological Process | protein glycosylation | |
Biological Process | sialylation |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameBeta-galactoside alpha-2,6-sialyltransferase 2
- EC number
- Short namesAlpha 2,6-ST 2
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ96JF0
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-11 | Cytoplasmic | ||||
Sequence: MKPHLKQWRQR | ||||||
Transmembrane | 12-32 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: MLFGIFAWGLLFLLIFIYFTD | ||||||
Topological domain | 33-529 | Lumenal | ||||
Sequence: SNPAEPVPSSLSFLETRRLLPVQGKQRAIMGAAHEPSPPGGLDARQALPRAHPAGSFHAGPGDLQKWAQSQDGFEHKEFFSSQVGRKSQSAFYPEDDDYFFAAGQPGWHSHTQGTLGFPSPGEPGPREGAFPAAQVQRRRVKKRHRRQRRSHVLEEGDDGDRLYSSMSRAFLYRLWKGNVSSKMLNPRLQKAMKDYLTANKHGVRFRGKREAGLSRAQLLCQLRSRARVRTLDGTEAPFSALGWRRLVPAVPLSQLHPRGLRSCAVVMSAGAILNSSLGEEIDSHDAVLRFNSAPTRGYEKDVGNKTTIRIINSQILTNPSHHFIDSSLYKDVILVAWDPAPYSANLNLWYKKPDYNLFTPYIQHRQRNPNQPFYILHPKFIWQLWDIIQENTKEKIQPNPPSSGFIGILIMMSMCREVHVYEYIPSVRQTELCHYHELYYDAACTLGAYHPLLYEKLLVQRLNMGTQGDLHRKGKVVLPGFQAVHCPAPSPVIPHS |
Keywords
- Cellular component
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_038046 | 154 | in dbSNP:rs3796110 | |||
Sequence: G → R | ||||||
Natural variant | VAR_038047 | 341 | in dbSNP:rs12615112 | |||
Sequence: I → V |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 889 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, glycosylation, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000314785 | 1-529 | Beta-galactoside alpha-2,6-sialyltransferase 2 | |||
Sequence: MKPHLKQWRQRMLFGIFAWGLLFLLIFIYFTDSNPAEPVPSSLSFLETRRLLPVQGKQRAIMGAAHEPSPPGGLDARQALPRAHPAGSFHAGPGDLQKWAQSQDGFEHKEFFSSQVGRKSQSAFYPEDDDYFFAAGQPGWHSHTQGTLGFPSPGEPGPREGAFPAAQVQRRRVKKRHRRQRRSHVLEEGDDGDRLYSSMSRAFLYRLWKGNVSSKMLNPRLQKAMKDYLTANKHGVRFRGKREAGLSRAQLLCQLRSRARVRTLDGTEAPFSALGWRRLVPAVPLSQLHPRGLRSCAVVMSAGAILNSSLGEEIDSHDAVLRFNSAPTRGYEKDVGNKTTIRIINSQILTNPSHHFIDSSLYKDVILVAWDPAPYSANLNLWYKKPDYNLFTPYIQHRQRNPNQPFYILHPKFIWQLWDIIQENTKEKIQPNPPSSGFIGILIMMSMCREVHVYEYIPSVRQTELCHYHELYYDAACTLGAYHPLLYEKLLVQRLNMGTQGDLHRKGKVVLPGFQAVHCPAPSPVIPHS | ||||||
Glycosylation | 69 | O-linked (GalNAc...) serine | ||||
Sequence: S | ||||||
Glycosylation | 211 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 253↔519 | |||||
Sequence: CQLRSRARVRTLDGTEAPFSALGWRRLVPAVPLSQLHPRGLRSCAVVMSAGAILNSSLGEEIDSHDAVLRFNSAPTRGYEKDVGNKTTIRIINSQILTNPSHHFIDSSLYKDVILVAWDPAPYSANLNLWYKKPDYNLFTPYIQHRQRNPNQPFYILHPKFIWQLWDIIQENTKEKIQPNPPSSGFIGILIMMSMCREVHVYEYIPSVRQTELCHYHELYYDAACTLGAYHPLLYEKLLVQRLNMGTQGDLHRKGKVVLPGFQAVHC | ||||||
Disulfide bond | 296↔448 | |||||
Sequence: CAVVMSAGAILNSSLGEEIDSHDAVLRFNSAPTRGYEKDVGNKTTIRIINSQILTNPSHHFIDSSLYKDVILVAWDPAPYSANLNLWYKKPDYNLFTPYIQHRQRNPNQPFYILHPKFIWQLWDIIQENTKEKIQPNPPSSGFIGILIMMSMC | ||||||
Disulfide bond | 466↔477 | |||||
Sequence: CHYHELYYDAAC |
Post-translational modification
O-glycosylated.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Weakly expressed in some tissues, such as small intestine, colon and fetal brain.
Induction
By IL6/interleukin-6 and IL8//interleukin-8.
Gene expression databases
Organism-specific databases
Interaction
Binary interactions
Protein-protein interaction databases
Miscellaneous
Structure
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q96JF0-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length529
- Mass (Da)60,158
- Last updated2008-01-15 v2
- ChecksumC3647E6DC0A9EFE3
Q96JF0-2
- Name2
- Differences from canonical
- 441-529: ILIMMSMCREVHVYEYIPSVRQTELCHYHELYYDAACTLGAYHPLLYEKLLVQRLNMGTQGDLHRKGKVVLPGFQAVHCPAPSPVIPHS → SFVKIGHIRACSEPRSRDCTPAWTTE
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Sequence caution
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_030356 | 441-529 | in isoform 2 | |||
Sequence: ILIMMSMCREVHVYEYIPSVRQTELCHYHELYYDAACTLGAYHPLLYEKLLVQRLNMGTQGDLHRKGKVVLPGFQAVHCPAPSPVIPHS → SFVKIGHIRACSEPRSRDCTPAWTTE |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB059555 EMBL· GenBank· DDBJ | BAC24793.1 EMBL· GenBank· DDBJ | mRNA | ||
AB058780 EMBL· GenBank· DDBJ | BAB47506.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AC016994 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AC108049 EMBL· GenBank· DDBJ | AAY15022.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH471127 EMBL· GenBank· DDBJ | EAX01742.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH471127 EMBL· GenBank· DDBJ | EAX01743.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC008680 EMBL· GenBank· DDBJ | AAH08680.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AJ512141 EMBL· GenBank· DDBJ | CAD54408.1 EMBL· GenBank· DDBJ | mRNA |