Q96HD9 · ACY3_HUMAN
- ProteinN-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming)
- GeneACY3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids319 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plays an important role in deacetylating mercapturic acids in kidney proximal tubules. Also acts on N-acetyl-aromatic amino acids (By similarity).
Catalytic activity
- an N-acyl-aromatic L-alpha-amino acid + H2O = a carboxylate + an aromatic L-alpha-amino acid
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 21 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 24 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 63 | substrate | ||||
Sequence: R | ||||||
Binding site | 70-71 | substrate | ||||
Sequence: NR | ||||||
Binding site | 116 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 178 | substrate | ||||
Sequence: E | ||||||
Binding site | 288 | substrate | ||||
Sequence: Y |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | apical plasma membrane | |
Cellular Component | cytosol | |
Cellular Component | extracellular exosome | |
Molecular Function | aminoacylase activity | |
Molecular Function | hydrolase activity, acting on ester bonds | |
Molecular Function | identical protein binding | |
Molecular Function | metal ion binding |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameN-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming)
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ96HD9
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Apical cell membrane ; Peripheral membrane protein
Note: Predominantly localized in the apical membrane of cells in the S1 segment. In the proximal straight tubules (S2 and S3 segments) is expressed diffusely throughout the cytoplasm.
Keywords
- Cellular component
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_048341 | 8 | in dbSNP:rs948445 | |||
Sequence: R → Q | ||||||
Natural variant | VAR_048342 | 281 | in dbSNP:rs2290959 | |||
Sequence: V → M |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 429 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000216875 | 1-319 | N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming) | |||
Sequence: MCSLPVPREPLRRVAVTGGTHGNEMSGVYLARHWLHAPAELQRASFSAVPVLANPAATSGCRRYVDHDLNRTFTSSFLNSRPTPDDPYEVTRARELNQLLGPKASGQAFDFVLDLHNTTANMGTCLIAKSSHEVFAMHLCRHLQLQYPELSCQVFLYQRSGEESYNLDSVAKNGLGLELGPQPQGVLRADIFSRMRTLVATVLDFIELFNQGTAFPAFEMEAYRPVGVVDFPRTEAGHLAGTVHPQLQDRDFQPLQPGAPIFQMFSGEDLLYEGESTVYPVFINEAAYYEKGVAFVQTEKFTFTVPAMPALTPAPSPAS |
Proteomic databases
PTM databases
Expression
Interaction
Subunit
Exists as a mixture of homodimers and homotetramer, both catalytically active.
(Microbial infection) Interacts with hepatitis C virus/HCV core protein.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q96HD9 | AAMDC Q9H7C9 | 3 | EBI-3916242, EBI-10308705 | |
BINARY | Q96HD9 | ARF5 P84085 | 3 | EBI-3916242, EBI-4289908 | |
BINARY | Q96HD9 | ASPA P45381 | 15 | EBI-3916242, EBI-750475 | |
BINARY | Q96HD9 | CYSRT1 A8MQ03 | 3 | EBI-3916242, EBI-3867333 | |
BINARY | Q96HD9 | DDIT4L Q96D03 | 5 | EBI-3916242, EBI-742054 | |
BINARY | Q96HD9 | GORASP2 Q9H8Y8 | 15 | EBI-3916242, EBI-739467 | |
BINARY | Q96HD9 | INCA1 Q0VD86 | 5 | EBI-3916242, EBI-6509505 | |
BINARY | Q96HD9 | KRTAP10-8 P60410 | 3 | EBI-3916242, EBI-10171774 | |
BINARY | Q96HD9 | KRTAP15-1 Q3LI76 | 3 | EBI-3916242, EBI-11992140 | |
BINARY | Q96HD9 | KRTAP26-1 Q6PEX3 | 3 | EBI-3916242, EBI-3957672 | |
BINARY | Q96HD9 | LNX1 Q8TBB1 | 3 | EBI-3916242, EBI-739832 | |
BINARY | Q96HD9 | NOTCH2NLA Q7Z3S9 | 3 | EBI-3916242, EBI-945833 | |
BINARY | Q96HD9 | PIAS2 O75928 | 3 | EBI-3916242, EBI-348555 | |
BINARY | Q96HD9 | PLEKHF2 Q9H8W4 | 4 | EBI-3916242, EBI-742388 | |
BINARY | Q96HD9 | PMM2 O15305 | 9 | EBI-3916242, EBI-10182608 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-210 | Hydrolytic domain | ||||
Sequence: MCSLPVPREPLRRVAVTGGTHGNEMSGVYLARHWLHAPAELQRASFSAVPVLANPAATSGCRRYVDHDLNRTFTSSFLNSRPTPDDPYEVTRARELNQLLGPKASGQAFDFVLDLHNTTANMGTCLIAKSSHEVFAMHLCRHLQLQYPELSCQVFLYQRSGEESYNLDSVAKNGLGLELGPQPQGVLRADIFSRMRTLVATVLDFIELFN | ||||||
Region | 211-318 | Shielding domain | ||||
Sequence: QGTAFPAFEMEAYRPVGVVDFPRTEAGHLAGTVHPQLQDRDFQPLQPGAPIFQMFSGEDLLYEGESTVYPVFINEAAYYEKGVAFVQTEKFTFTVPAMPALTPAPSPA |
Sequence similarities
Belongs to the AspA/AstE family. Aspartoacylase subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length319
- Mass (Da)35,241
- Last updated2001-12-01 v1
- Checksum2595358AC8B5BEFB
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
E9PRA7 | E9PRA7_HUMAN | ACY3 | 198 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY040761 EMBL· GenBank· DDBJ | AAK94770.1 EMBL· GenBank· DDBJ | mRNA | ||
AY169233 EMBL· GenBank· DDBJ | AAN87896.1 EMBL· GenBank· DDBJ | mRNA | ||
BC008689 EMBL· GenBank· DDBJ | AAH08689.1 EMBL· GenBank· DDBJ | mRNA |