Q96GA7 · SDSL_HUMAN
- ProteinSerine dehydratase-like
- GeneSDSL
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids329 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the pyridoxal-phosphate-dependent dehydrative deamination of L-threonine and L-serine to ammonia and alpha-ketobutyrate and pyruvate, respectively (PubMed:16580895, PubMed:18342636).
Also exhibits racemase activity towards L-glutamate and D-glutamate (By similarity).
Also exhibits racemase activity towards L-glutamate and D-glutamate (By similarity).
Catalytic activity
- L-serine = NH4+ + pyruvate
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
30 mM | L-serine | |||||
32.2 mM | L-serine | |||||
7 mM | L-threonine | |||||
3.1 mM | L-threonine |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
7.8 nmol/min/mg | for L-serine | ||||
1.8 nmol/min/mg | for L-threonine |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | glutamate racemase activity | |
Molecular Function | identical protein binding | |
Molecular Function | L-serine ammonia-lyase activity | |
Molecular Function | pyridoxal phosphate binding | |
Molecular Function | threonine deaminase activity | |
Biological Process | isoleucine biosynthetic process | |
Biological Process | L-serine catabolic process | |
Biological Process | lipid metabolic process | |
Biological Process | threonine catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSerine dehydratase-like
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ96GA7
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Disease & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 72 | Strongly increased enzyme activity towards threonine. | ||||
Sequence: G → A | ||||||
Mutagenesis | 287 | Almost no change in Km and Vmax for serine and threonine. Significantly increased protein levels. | ||||
Sequence: L → V | ||||||
Mutagenesis | 309 | Loss of enzyme activity. | ||||
Sequence: C → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 461 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 1 | N-acetylmethionine | ||||
Sequence: M | ||||||
Chain | PRO_0000264624 | 1-329 | Serine dehydratase-like | |||
Sequence: MDGPVAEHAKQEPFHVVTPLLESWALSQVAGMPVFLKCENVQPSGSFKIRGIGHFCQEMAKKGCRHLVCSSGGNAGIAAAYAARKLGIPATIVLPESTSLQVVQRLQGEGAEVQLTGKVWDEANLRAQELAKRDGWENVPPFDHPLIWKGHASLVQELKAVLRTPPGALVLAVGGGGLLAGVVAGLLEVGWQHVPIIAMETHGAHCFNAAITAGKLVTLPDITSVAKSLGAKTVAARALECMQVCKIHSEVVEDTEAVSAVQQLLDDERMLVEPACGAALAAIYSGLLRRLQAEGCLPPSLTSVVVIVCGGNNINSRELQALKTHLGQV | ||||||
Modified residue | 48 | N6-(pyridoxal phosphate)lysine | ||||
Sequence: K |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in lung cancer cell lines.
Gene expression databases
Organism-specific databases
Interaction
Subunit
Monomer (By similarity).
Homodimer
Homodimer
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q96GA7 | SDSL Q96GA7 | 6 | EBI-744440, EBI-744440 |
Protein-protein interaction databases
Miscellaneous
Structure
Sequence
- Sequence statusComplete
- Length329
- Mass (Da)34,674
- Last updated2001-12-01 v1
- ChecksumCEAD30E570A43B7E
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF134473 EMBL· GenBank· DDBJ | AAP97250.1 EMBL· GenBank· DDBJ | mRNA | ||
BC009849 EMBL· GenBank· DDBJ | AAH09849.1 EMBL· GenBank· DDBJ | mRNA | ||
BC091479 EMBL· GenBank· DDBJ | AAH91479.1 EMBL· GenBank· DDBJ | mRNA |