Q96F10 · SAT2_HUMAN
- ProteinThialysine N-epsilon-acetyltransferase
- GeneSAT2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids170 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the N-acetylation of the amino acid thialysine (S-(2-aminoethyl)-L-cysteine), a L-lysine analog with the 4-methylene group substituted with a sulfur (PubMed:15283699).
May also catalyze acetylation of polyamines, such as norspermidine, spermidine or spermine (PubMed:12803540).
However, ability to acetylate polyamines is weak, suggesting that it does not act as a diamine acetyltransferase in vivo (PubMed:15283699).
May also catalyze acetylation of polyamines, such as norspermidine, spermidine or spermine (PubMed:12803540).
However, ability to acetylate polyamines is weak, suggesting that it does not act as a diamine acetyltransferase in vivo (PubMed:15283699).
Catalytic activity
- acetyl-CoA + S-(2-aminoethyl)-L-cysteine = CoA + H+ + S-(2-acetamidoethyl)-L-cysteineThis reaction proceeds in the forward direction.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
8.2 mM | putrescine | |||||
13.4 mM | spermidine | |||||
4.8 mM | spermine | |||||
1.6 mM | 1,3-diaminopropane | |||||
6 mM | norspermidine | |||||
0.29 mM | thialysine |
kcat is 0.0023 sec-1 with putrescine as substrate (PubMed:15283699).
kcat is 0.0072 sec-1 with spermidine as substrate (PubMed:15283699).
kcat is 0.0020 sec-1 with spermine as substrate (PubMed:15283699).
kcat is 0.0137 sec-1 with 1,3-diaminopropane as substrate (PubMed:15283699).
kcat is 5.25 sec-1 with thialysine as substrate (PubMed:15283699).
kcat is 0.0072 sec-1 with spermidine as substrate (PubMed:15283699).
kcat is 0.0020 sec-1 with spermine as substrate (PubMed:15283699).
kcat is 0.0137 sec-1 with 1,3-diaminopropane as substrate (PubMed:15283699).
kcat is 5.25 sec-1 with thialysine as substrate (PubMed:15283699).
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 27-28 | substrate | ||||
Sequence: FE | ||||||
Binding site | 92 | substrate | ||||
Sequence: E | ||||||
Binding site | 94-96 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: IYV | ||||||
Binding site | 102-107 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: GQGIGS | ||||||
Binding site | 133-135 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: NQR | ||||||
Active site | 140 | Proton donor | ||||
Sequence: Y | ||||||
Binding site | 140 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 152 | substrate | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | extracellular exosome | |
Molecular Function | diamine N-acetyltransferase activity | |
Molecular Function | identical protein binding | |
Molecular Function | N-acetyltransferase activity | |
Biological Process | nor-spermidine metabolic process | |
Biological Process | putrescine acetylation | |
Biological Process | spermidine acetylation | |
Biological Process | spermine acetylation |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThialysine N-epsilon-acetyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ96F10
Proteomes
Organism-specific databases
Subcellular Location
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_020465 | 126 | in dbSNP:rs13894 | |||
Sequence: R → C |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 179 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000074598 | 1-170 | Thialysine N-epsilon-acetyltransferase | |||
Sequence: MASVRIREAKEGDCGDILRLIRELAEFEKLSDQVKISEEALRADGFGDNPFYHCLVAEILPAPGKLLGPCVVGYGIYYFIYSTWKGRTIYLEDIYVMPEYRGQGIGSKIIKKVAEVALDKGCSQFRLAVLDWNQRAMDLYKALGAQDLTEAEGWHFFCFQGEATRKLAGK | ||||||
Modified residue | 29 | N6-acetyllysine | ||||
Sequence: K |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Widely expressed (PubMed:12803540, PubMed:15283699).
Under physiological conditions, SSAT2 is expressed at lower level that SSAT1 (SSAT). Many tissues express only SSAT1, several tissues express both SSAT1 and SSAT2, and bone, cervix, ovary and pineal gland expressed only SSAT2 (PubMed:12803540).
Under physiological conditions, SSAT2 is expressed at lower level that SSAT1 (SSAT). Many tissues express only SSAT1, several tissues express both SSAT1 and SSAT2, and bone, cervix, ovary and pineal gland expressed only SSAT2 (PubMed:12803540).
Induction
Not inducible by polyamine analogs.
Gene expression databases
Organism-specific databases
Interaction
Subunit
Homodimer.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q96F10 | SAT1 P21673 | 6 | EBI-748746, EBI-711613 | |
BINARY | Q96F10 | SAT2 Q96F10 | 5 | EBI-748746, EBI-748746 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 4-168 | N-acetyltransferase | ||||
Sequence: VRIREAKEGDCGDILRLIRELAEFEKLSDQVKISEEALRADGFGDNPFYHCLVAEILPAPGKLLGPCVVGYGIYYFIYSTWKGRTIYLEDIYVMPEYRGQGIGSKIIKKVAEVALDKGCSQFRLAVLDWNQRAMDLYKALGAQDLTEAEGWHFFCFQGEATRKLA |
Sequence similarities
Belongs to the acetyltransferase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length170
- Mass (Da)19,155
- Last updated2001-12-01 v1
- Checksum68D46F0E28186C68
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF348524 EMBL· GenBank· DDBJ | AAL83905.1 EMBL· GenBank· DDBJ | mRNA | ||
BC011751 EMBL· GenBank· DDBJ | AAH11751.1 EMBL· GenBank· DDBJ | mRNA |