Q969S8 · HDA10_HUMAN
- ProteinPolyamine deacetylase HDAC10
- GeneHDAC10
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids669 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Exhibits attenuated catalytic activity toward N1,N8-diacetylspermidine and very low activity, if any, toward N1-acetylspermidine (PubMed:28516954).
Histone deacetylase activity has been observed in vitro (PubMed:11677242, PubMed:11726666, PubMed:11739383, PubMed:11861901).
Has also been shown to be involved in MSH2 deacetylation (PubMed:26221039).
The physiological relevance of protein/histone deacetylase activity is unclear and could be very weak (PubMed:28516954).
May play a role in the promotion of late stages of autophagy, possibly autophagosome-lysosome fusion and/or lysosomal exocytosis in neuroblastoma cells (PubMed:23801752, PubMed:29968769).
May play a role in homologous recombination (PubMed:21247901).
May promote DNA mismatch repair (PubMed:26221039).
Miscellaneous
Catalytic activity
- H2O + N8-acetylspermidine = acetate + spermidine
- H2O + N-acetylcadaverine = acetate + cadaverine
- H2O + N6-acetyl-L-lysyl-[protein] = acetate + L-lysyl-[protein]
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
110 μM | acetylcadaverine | |||||
170 μM | acetylputrescine | |||||
100 μM | N8-acetylspermidine | |||||
180 μM | N1-acetylspermine | |||||
150 μM | N1,N8-diacetylspermidine |
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 20 | substrate | ||||
Sequence: D | ||||||
Active site | 135 | Proton donor/acceptor | ||||
Sequence: H | ||||||
Binding site | 172 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 174 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 265 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Site | 272 | Substrate specificity | ||||
Sequence: E | ||||||
Binding site | 305 | substrate | ||||
Sequence: Y |
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePolyamine deacetylase HDAC10
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ969S8
- Secondary accessions
Proteomes
Organism-specific databases
Disease & Variants
Involvement in disease
Features
Showing features for mutagenesis, natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 135 | Abolishes deacetylase activity. Does not affect interaction with HDAC3. Loss of autophagy regulation. | ||||
Sequence: H → A | ||||||
Natural variant | VAR_049356 | 429 | in dbSNP:rs34402301 | |||
Sequence: V → I |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 867 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data), modified residue.
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000114712 | 1-669 | UniProt | Polyamine deacetylase HDAC10 | |||
Sequence: MGTALVYHEDMTATRLLWDDPECEIERPERLTAALDRLRQRGLEQRCLRLSAREASEEELGLVHSPEYVSLVRETQVLGKEELQALSGQFDAIYFHPSTFHCARLAAGAGLQLVDAVLTGAVQNGLALVRPPGHHGQRAAANGFCVFNNVAIAAAHAKQKHGLHRILVVDWDVHHGQGIQYLFEDDPSVLYFSWHRYEHGRFWPFLRESDADAVGRGQGLGFTVNLPWNQVGMGNADYVAAFLHLLLPLAFEFDPELVLVSAGFDSAIGDPEGQMQATPECFAHLTQLLQVLAGGRVCAVLEGGYHLESLAESVCMTVQTLLGDPAPPLSGPMAPCQSALESIQSARAAQAPHWKSLQQQDVTAVPMSPSSHSPEGRPPPLLPGGPVCKAAASAPSSLLDQPCLCPAPSVRTAVALTTPDITLVLPPDVIQQEASALREETEAWARPHESLAREEALTALGKLLYLLDGMLDGQVNSGIAATPASAAAATLDVAVRRGLSHGAQRLLCVALGQLDRPPDLAHDGRSLWLNIRGKEAAALSMFHVSTPLPVMTGGFLSCILGLVLPLAYGFQPDLVLVALGPGHGLQGPHAALLAAMLRGLAGGRVLALLEENSTPQLAGILARVLNGEAPPSLGPSSVASPEDVQALMYLRGQLEPQWKMLQCHPHLVA | |||||||
Modified residue (large scale data) | 368 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue | 393 | UniProt | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Gene expression databases
Organism-specific databases
Interaction
Subunit
Interacts with HDAC2 and NCOR2/SMRT (PubMed:11739383).
Interacts with HSPA8/HSC70 (PubMed:23801752).
Interacts with MSH2 (PubMed:26221039).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q969S8 | APBB2 Q92870-2 | 3 | EBI-301762, EBI-21535880 | |
BINARY | Q969S8 | ATXN3 P54252 | 3 | EBI-301762, EBI-946046 | |
BINARY | Q969S8 | HTT P42858 | 21 | EBI-301762, EBI-466029 | |
BINARY | Q969S8 | OPTN Q96CV9 | 3 | EBI-301762, EBI-748974 | |
BINARY | Q969S8 | PEX26 Q7Z412 | 3 | EBI-301762, EBI-752057 | |
BINARY | Q969S8 | PMP22 A0A6Q8PF08 | 3 | EBI-301762, EBI-50433196 | |
BINARY | Q969S8 | SNCA P37840 | 3 | EBI-301762, EBI-985879 | |
BINARY | Q969S8 | TRIM54 Q9BYV2 | 2 | EBI-301762, EBI-2130429 |
Protein-protein interaction databases
Chemistry
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-323 | Histone deacetylase | ||||
Sequence: MGTALVYHEDMTATRLLWDDPECEIERPERLTAALDRLRQRGLEQRCLRLSAREASEEELGLVHSPEYVSLVRETQVLGKEELQALSGQFDAIYFHPSTFHCARLAAGAGLQLVDAVLTGAVQNGLALVRPPGHHGQRAAANGFCVFNNVAIAAAHAKQKHGLHRILVVDWDVHHGQGIQYLFEDDPSVLYFSWHRYEHGRFWPFLRESDADAVGRGQGLGFTVNLPWNQVGMGNADYVAAFLHLLLPLAFEFDPELVLVSAGFDSAIGDPEGQMQATPECFAHLTQLLQVLAGGRVCAVLEGGYHLESLAESVCMTVQTLLG | ||||||
Motif | 21-24 | Substrate specificity | ||||
Sequence: PECE | ||||||
Region | 361-387 | Disordered | ||||
Sequence: DVTAVPMSPSSHSPEGRPPPLLPGGPV |
Sequence similarities
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 4 isoforms produced by Alternative splicing.
Q969S8-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- SynonymsAlpha, HDAC10b, HDAC10v1
- Length669
- Mass (Da)71,445
- Last updated2001-12-01 v1
- Checksum872D9427E6893A18
Q969S8-2
- Name2
- SynonymsBeta
- Differences from canonical
- 252-271: Missing
Q969S8-4
- Name4
- SynonymsA, HDAC10v2
- NoteMay be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
- Differences from canonical
- 612-669: NSTPQLAGILARVLNGEAPPSLGPSSVASPEDVQALMYLRGQLEPQWKMLQCHPHLVA → VSWAGWRCCGVGRGKGPVTASVFAPGPELHTPASRDPGPGAEWRGTS
Q969S8-5
- Name5
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Features
Showing features for sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 92 | in Ref. 6; AAS48345 | ||||
Sequence: A → T | ||||||
Sequence conflict | 177 | in Ref. 6; AAS48345 | ||||
Sequence: Q → R | ||||||
Alternative sequence | VSP_002089 | 252-271 | in isoform 2 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_014698 | 252-301 | in isoform 5 | |||
Sequence: Missing | ||||||
Sequence conflict | 337 | in Ref. 9; CAC21653 | ||||
Sequence: Q → QRC | ||||||
Sequence conflict | 337 | in Ref. 4; no nucleotide entry | ||||
Sequence: Q → QRCEG | ||||||
Alternative sequence | VSP_014699 | 447-669 | in isoform 5 | |||
Sequence: Missing | ||||||
Sequence conflict | 594 | in Ref. 3; AAK92205/AAK92206 | ||||
Sequence: A → T | ||||||
Alternative sequence | VSP_002090 | 612-669 | in isoform 4 | |||
Sequence: NSTPQLAGILARVLNGEAPPSLGPSSVASPEDVQALMYLRGQLEPQWKMLQCHPHLVA → VSWAGWRCCGVGRGKGPVTASVFAPGPELHTPASRDPGPGAEWRGTS |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF426160 EMBL· GenBank· DDBJ | AAL30513.1 EMBL· GenBank· DDBJ | mRNA | ||
AF393962 EMBL· GenBank· DDBJ | AAK84023.1 EMBL· GenBank· DDBJ | mRNA | ||
AF407272 EMBL· GenBank· DDBJ | AAK92205.1 EMBL· GenBank· DDBJ | mRNA | ||
AF407273 EMBL· GenBank· DDBJ | AAK92206.1 EMBL· GenBank· DDBJ | mRNA | ||
CR456465 EMBL· GenBank· DDBJ | CAG30351.1 EMBL· GenBank· DDBJ | mRNA | ||
AY450395 EMBL· GenBank· DDBJ | AAS48345.1 EMBL· GenBank· DDBJ | mRNA | ||
AL022328 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC125083 EMBL· GenBank· DDBJ | AAI25084.1 EMBL· GenBank· DDBJ | mRNA | ||
AL512711 EMBL· GenBank· DDBJ | CAC21653.2 EMBL· GenBank· DDBJ | mRNA |