Q965X7 · NAPE1_CAEEL

  • Protein
    N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D 1
  • Gene
    nape-1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

D-type phospholipase that hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce bioactive N-acylethanolamines/fatty acid ethanolamides (NAEs/FAEs) and phosphatidic acid (PubMed:25423491).
NAEs are bioactive lipids that are involved in diverse physiological processes such as growth and lifespan (PubMed:21562563, PubMed:25423491).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 zinc divalent cations per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site144Zn2+ 1 (UniProtKB | ChEBI)
Binding site146Zn2+ 1 (UniProtKB | ChEBI)
Binding site147N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine (UniProtKB | ChEBI)
Binding site148Zn2+ 2 (UniProtKB | ChEBI)
Binding site149Zn2+ 2 (UniProtKB | ChEBI)
Binding site217Zn2+ 1 (UniProtKB | ChEBI)
Binding site248Zn2+ 1 (UniProtKB | ChEBI)
Binding site248Zn2+ 2 (UniProtKB | ChEBI)
Binding site286N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine (UniProtKB | ChEBI)
Binding site308Zn2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentmembrane-bounded organelle
Cellular Componentneuronal cell body
Molecular FunctionN-acylphosphatidylethanolamine-specific phospholipase D activity
Molecular Functionzinc ion binding
Biological ProcessN-acylethanolamine metabolic process
Biological ProcessN-acylphosphatidylethanolamine metabolic process
Biological Processphospholipid catabolic process

Keywords

Enzyme and pathway databases

Chemistry

Names & Taxonomy

Protein names

  • Recommended name
    N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D 1
  • EC number
  • Short names
    NAPE-1

Gene names

    • Name
      nape-1
    • ORF names
      Y37E11AR.4

Organism names

  • Taxonomic identifier
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    Q965X7

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

No strong phenotype in the nape-1 deletion mutant, however its overexpression alters growth and lifespan when grown at 25 degrees Celsius compared to 15 degrees Celsius.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004527421-356N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D 1

Proteomic databases

Expression

Tissue specificity

Expressed in interneurons that are in close proximity to the primary sensory neurons (PubMed:21562563).
Predominantly expressed in the pharynx but can also be found in cell bodies of the dorsal and ventral nerve cords (PubMed:25423491).

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the NAPE-PLD family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    356
  • Mass (Da)
    40,319
  • Last updated
    2001-12-01 v1
  • Checksum
    F3EE650FAEE98103
MSLPTTSSPLTNDQEFAKPVRNGKIFENPKSFTNWGGRPGLTNIFKLVLRETSHENLPSDKKVLDSTLPVHNITADDFHSESGLFATWLGHATVLVDLEGVKFVTDPVWADRASFTSFAGPKRYRPPPMKLEDLPDLDFAVVSHDHYDHLDADAVKRITNLNPQIKWFVPLGLKKWMKNQGIGADGSNTVTELNWGESSEFVKNGKTITIWCLPAQHSGQRGLSDHNHRLWSGWAVIGENRRFYFPGDTGFCDVEFKKIGEKLGPFDLAAIPIGAYEPRWFMKSHHINPDEAVEVHKLVRARNSIGIHWGTYPMGTTEYYLEPRDKLKELMDAREDLKDTSFVTVDMGEIWEASDR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BX284604
EMBL· GenBank· DDBJ
CCD74042.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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