Q962P8 · TBA6_NAEPR
- ProteinTubulin alpha-6 chain
- GeneTUBA6
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids452 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Catalytic activity
- GTP + H2O = GDP + phosphate + H+This reaction proceeds in the forward direction.
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 11 | GTP (UniProtKB | ChEBI) | |||
Binding site | 69 | GTP (UniProtKB | ChEBI) | |||
Binding site | 69 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 138 | GTP (UniProtKB | ChEBI) | |||
Binding site | 142 | GTP (UniProtKB | ChEBI) | |||
Binding site | 143 | GTP (UniProtKB | ChEBI) | |||
Binding site | 177 | GTP (UniProtKB | ChEBI) | |||
Binding site | 204 | GTP (UniProtKB | ChEBI) | |||
Binding site | 226 | GTP (UniProtKB | ChEBI) | |||
Active site | 252 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | microtubule | |
Cellular Component | spindle | |
Molecular Function | GTP binding | |
Molecular Function | hydrolase activity | |
Molecular Function | metal ion binding | |
Molecular Function | structural constituent of cytoskeleton | |
Biological Process | microtubule cytoskeleton organization | |
Biological Process | mitotic cell cycle |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTubulin alpha-6 chain
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Discoba > Heterolobosea > Tetramitia > Eutetramitia > Vahlkampfiidae > Naegleria
Accessions
- Primary accessionQ962P8
Subcellular Location
UniProt Annotation
GO Annotation
Note: Present in the nuclei and mitotic spindle-fibers but absent in flagellar axonemes or cytoskeletal microtubules.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000048196 | 1-452 | Tubulin alpha-6 chain | ||
Expression
Developmental stage
Expressed in actively growing cells and repressed quickly when these cells were induced to differentiate.
Interaction
Subunit
Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.
Structure
Sequence
- Sequence statusComplete
- Length452
- Mass (Da)50,470
- Last updated2001-12-01 v1
- MD5 Checksum3CE98F19B8E249FF31FD2A38074B7077
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF401640 EMBL· GenBank· DDBJ | AAK84065.1 EMBL· GenBank· DDBJ | Genomic DNA |