Q95Y80 · BET1_CAEEL
- ProteinBromodomain-containing protein bet-1
- Genebet-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids853 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Required for the establishment and maintenance of stable cell fate in several lineages including V5.pa, T, Z1/Z4 and QR lineages probably by repressing the expression of cell fate determinants (PubMed:20181741, PubMed:24346701).
Required to maintain non-distal tip cell (DTC) fate of somatic gonadal cells through the htz-1-mediated repression of transcription factor ceh-22. Regulates the subnuclear localization of histone variant htz-1 in somatic gonadal cells (PubMed:24346701).
Plays a role in the attenuation of the let-60/ras pathway, probably by preventing expression of activators of the pathway (PubMed:24285704, PubMed:24349540).
Involved in adult locomotion. Acts together with the sumoylation pathway to prevent muscle myosin depletion in aging adults probably by preventing myoblast growth factor receptor egl-15 overexpression (PubMed:24285704).
May play a role in vulva development (PubMed:24349540).
Required to maintain non-distal tip cell (DTC) fate of somatic gonadal cells through the htz-1-mediated repression of transcription factor ceh-22. Regulates the subnuclear localization of histone variant htz-1 in somatic gonadal cells (PubMed:24346701).
Plays a role in the attenuation of the let-60/ras pathway, probably by preventing expression of activators of the pathway (PubMed:24285704, PubMed:24349540).
Involved in adult locomotion. Acts together with the sumoylation pathway to prevent muscle myosin depletion in aging adults probably by preventing myoblast growth factor receptor egl-15 overexpression (PubMed:24285704).
May play a role in vulva development (PubMed:24349540).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chromatin | |
Cellular Component | chromosome | |
Cellular Component | metaphase plate | |
Cellular Component | nucleus | |
Molecular Function | lysine-acetylated histone binding | |
Molecular Function | SUMO binding | |
Biological Process | cell fate specification | |
Biological Process | chromatin remodeling | |
Biological Process | multicellular organismal locomotion | |
Biological Process | muscle cell cellular homeostasis | |
Biological Process | negative regulation of cell fate specification | |
Biological Process | negative regulation of DNA-templated transcription | |
Biological Process | neuroblast fate specification | |
Biological Process | protein localization to chromatin | |
Biological Process | regulation of DNA-templated transcription | |
Biological Process | regulation of protein stability | |
Biological Process | regulation of transcription by RNA polymerase II | |
Biological Process | regulation of vulval development |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBromodomain-containing protein bet-1
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ95Y80
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localizes to granular structures in interphase and co-localizes with chromosomes during metaphase.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Abnormal cell fate determination of several lineages. T.p daughter cells adopt a hypodermal cell fate instead of a neuronal cell fate resulting in the loss of the phasmid socket cell. Generation of ectopic PDE, AVM and PVM neurons, and distal tip cell (DTC) (PubMed:20181741, PubMed:24346701).
In addition, ectopic egl-17 expression in multiple vulva precursor cells and ectopic che-22 expression in extra distal tip cells (PubMed:24346701, PubMed:24349540).
Moderate increase in mpk-1 phosphorylation (PubMed:24349540).
RNAi-mediated knockdown further suppresses the age-dependent paralysis phenotype of the spop-1 gk630214 or dr95 mutants (PubMed:34593637).
In addition, ectopic egl-17 expression in multiple vulva precursor cells and ectopic che-22 expression in extra distal tip cells (PubMed:24346701, PubMed:24349540).
Moderate increase in mpk-1 phosphorylation (PubMed:24349540).
RNAi-mediated knockdown further suppresses the age-dependent paralysis phenotype of the spop-1 gk630214 or dr95 mutants (PubMed:34593637).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 113 | In os118; 50 percent of animals lack phasmid socket cell and have an ectopic distal tip cell; when associated with K-585. | ||||
Sequence: V → I | ||||||
Mutagenesis | 252 | Loss of interaction with smo-1 and ubc-9. | ||||
Sequence: K → R | ||||||
Mutagenesis | 253 | No effect on the interaction with smo-1 and ubc-9. | ||||
Sequence: K → R | ||||||
Mutagenesis | 264 | No effect on the interaction with smo-1; when associated with R-276 and R-277. | ||||
Sequence: K → R | ||||||
Mutagenesis | 276-277 | No effect on the interaction with smo-1; when associated with R-264. | ||||
Sequence: KK → RR | ||||||
Mutagenesis | 304 | No effect on the interaction with smo-1; when associated with R-313 and R-315. | ||||
Sequence: K → R | ||||||
Mutagenesis | 313 | No effect on the interaction with smo-1; when associated with R-304 and R-315. | ||||
Sequence: K → R | ||||||
Mutagenesis | 315 | No effect on the interaction with smo-1; when associated with R-304 and R-313. | ||||
Sequence: K → R | ||||||
Mutagenesis | 585 | In os118; 50 percent of animals lack phasmid socket cell and have an ectopic distal tip cell; when associated with I-113. | ||||
Sequence: E → K |
PTM/Processing
Features
Showing features for chain, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000434603 | 1-853 | Bromodomain-containing protein bet-1 | |||
Sequence: MSEGSGDQSQQRPWASPRQQPIKGIVQPRVLPPFGKPTRHTNKLDYIMTTVLKEAGKHKHVWPFQKPVDAVALCIPLYHERVARPMDLKTIENRLKSTYYTCAQECIDDIETVFQNCYTFNGKEDDVTIMAQNVHEVIKKSLEQAPREEHDMDVYWGKNKKKPAKSDGGSKSSSSKKNDARGPSEAPSEAGSEVSSVTTASAAAPTVSESASVAAKPERKVAGKKTGKRKAESEDDEKPEPLRAKREVAVVKKEVHQPLLPSMKPCLKLLNDFSTKKYQEFAWPFNEPVDAEQLGLHDYHKIIKEPMDLKSMKAKMESGAYKEPSDFEHDVRLMLRNCFLYNPVGDPVHSFGLRFQEVFDRRWAELGDSSSRASSVAPQSAPIAPTPKVAKSSAPKEPKESRKEHKKETTFEASGAKSEDLMQINNALSMIREREEKLKAELAAAQAIKDKLTSVKNRREDNPNEPFPEKLINETRALCTTQVGQNASSSSASSAALRNGRSKKAASARLYGYEFDSDDEDNKMALTYEEKRNLSNLINNLPNNQLNTIISIIQRRERSALMQQQLDDSEVELDFESLGDMCLREMGAFIKTIPTLNGNGDDEKPKTSSNPTSSGATGSKGSSSLESKNGKKKKNFNMSESSDDETSNSRKRRKRESSESQSSSSSDDDSDDEDRPSIPRKSGQPPSTSREWNQSSAPPPRMGGMGGQPPMSRVPASSSTSVSAIGKNNAAASSNSYQAPKPAPVPAPTSSRPPAAPRPPSKPKKTGGASILDTLLPDTFGASPPQFFQSQPTTSATIRSPTESQPGNGEDEQTRIQRMRMEAKRARQKEDEGSVSLSNQMEMMAAFEFDNTY | ||||||
Cross-link | 252 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) | ||||
Sequence: K |
Keywords
- PTM
Proteomic databases
Expression
Tissue specificity
Expressed in T-cells, Q-cells, V5-cells and their descendants such as somatic gonad and syncytium.
Gene expression databases
Interaction
Subunit
Interacts with acetylated histone H4 (PubMed:20181741).
Interacts (via BROMO domain 2) with smo-1 and ubc-9 (PubMed:24349540).
Interacts (via BROMO domain 2) with smo-1 and ubc-9 (PubMed:24349540).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q95Y80 | siah-1 Q965X6 | 2 | EBI-311872, EBI-311877 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-17 | Polar residues | ||||
Sequence: MSEGSGDQSQQRPWASP | ||||||
Region | 1-22 | Disordered | ||||
Sequence: MSEGSGDQSQQRPWASPRQQPI | ||||||
Domain | 56-128 | Bromo 1 | ||||
Sequence: GKHKHVWPFQKPVDAVALCIPLYHERVARPMDLKTIENRLKSTYYTCAQECIDDIETVFQNCYTFNGKEDDVT | ||||||
Compositional bias | 141-167 | Basic and acidic residues | ||||
Sequence: SLEQAPREEHDMDVYWGKNKKKPAKSD | ||||||
Region | 141-245 | Disordered | ||||
Sequence: SLEQAPREEHDMDVYWGKNKKKPAKSDGGSKSSSSKKNDARGPSEAPSEAGSEVSSVTTASAAAPTVSESASVAAKPERKVAGKKTGKRKAESEDDEKPEPLRAK | ||||||
Compositional bias | 189-208 | Polar residues | ||||
Sequence: EAGSEVSSVTTASAAAPTVS | ||||||
Compositional bias | 218-245 | Basic and acidic residues | ||||
Sequence: ERKVAGKKTGKRKAESEDDEKPEPLRAK | ||||||
Domain | 277-349 | Bromo 2 | ||||
Sequence: KYQEFAWPFNEPVDAEQLGLHDYHKIIKEPMDLKSMKAKMESGAYKEPSDFEHDVRLMLRNCFLYNPVGDPVH | ||||||
Region | 369-418 | Disordered | ||||
Sequence: SSSRASSVAPQSAPIAPTPKVAKSSAPKEPKESRKEHKKETTFEASGAKS | ||||||
Compositional bias | 393-414 | Basic and acidic residues | ||||
Sequence: SAPKEPKESRKEHKKETTFEAS | ||||||
Coiled coil | 419-458 | |||||
Sequence: EDLMQINNALSMIREREEKLKAELAAAQAIKDKLTSVKNR | ||||||
Domain | 516-601 | NET | ||||
Sequence: DSDDEDNKMALTYEEKRNLSNLINNLPNNQLNTIISIIQRRERSALMQQQLDDSEVELDFESLGDMCLREMGAFIKTIPTLNGNGD | ||||||
Region | 594-814 | Disordered | ||||
Sequence: PTLNGNGDDEKPKTSSNPTSSGATGSKGSSSLESKNGKKKKNFNMSESSDDETSNSRKRRKRESSESQSSSSSDDDSDDEDRPSIPRKSGQPPSTSREWNQSSAPPPRMGGMGGQPPMSRVPASSSTSVSAIGKNNAAASSNSYQAPKPAPVPAPTSSRPPAAPRPPSKPKKTGGASILDTLLPDTFGASPPQFFQSQPTTSATIRSPTESQPGNGEDEQT | ||||||
Compositional bias | 601-625 | Polar residues | ||||
Sequence: DDEKPKTSSNPTSSGATGSKGSSSL | ||||||
Compositional bias | 626-662 | Basic and acidic residues | ||||
Sequence: ESKNGKKKKNFNMSESSDDETSNSRKRRKRESSESQS | ||||||
Compositional bias | 681-696 | Polar residues | ||||
Sequence: KSGQPPSTSREWNQSS | ||||||
Compositional bias | 712-740 | Polar residues | ||||
Sequence: SRVPASSSTSVSAIGKNNAAASSNSYQAP | ||||||
Compositional bias | 741-761 | Pro residues | ||||
Sequence: KPAPVPAPTSSRPPAAPRPPS | ||||||
Compositional bias | 783-812 | Polar residues | ||||
Sequence: SPPQFFQSQPTTSATIRSPTESQPGNGEDE | ||||||
Region | 819-838 | Disordered | ||||
Sequence: MRMEAKRARQKEDEGSVSLS |
Domain
The BROMO domain 2 is essential for the interaction with smo-1 and E2 enzyme ubc-9.
Sequence similarities
Belongs to the BET family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q95Y80-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Namea
- Length853
- Mass (Da)94,086
- Last updated2012-01-25 v3
- Checksum7B4C4A18226A35A5
Q95Y80-2
- Nameb
Computationally mapped potential isoform sequences
There are 6 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A5S9MPT0 | A0A5S9MPT0_CAEEL | bet-1 | 857 | ||
A0A5S9MQE5 | A0A5S9MQE5_CAEEL | bet-1 | 854 | ||
A0A5S9MM65 | A0A5S9MM65_CAEEL | bet-1 | 852 | ||
A0A5S9MMD4 | A0A5S9MMD4_CAEEL | bet-1 | 852 | ||
A0A5S9MMP9 | A0A5S9MMP9_CAEEL | bet-1 | 855 | ||
A0A5S9MN31 | A0A5S9MN31_CAEEL | bet-1 | 855 |
Features
Showing features for compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-17 | Polar residues | ||||
Sequence: MSEGSGDQSQQRPWASP | ||||||
Compositional bias | 141-167 | Basic and acidic residues | ||||
Sequence: SLEQAPREEHDMDVYWGKNKKKPAKSD | ||||||
Compositional bias | 189-208 | Polar residues | ||||
Sequence: EAGSEVSSVTTASAAAPTVS | ||||||
Compositional bias | 218-245 | Basic and acidic residues | ||||
Sequence: ERKVAGKKTGKRKAESEDDEKPEPLRAK | ||||||
Compositional bias | 393-414 | Basic and acidic residues | ||||
Sequence: SAPKEPKESRKEHKKETTFEAS | ||||||
Compositional bias | 601-625 | Polar residues | ||||
Sequence: DDEKPKTSSNPTSSGATGSKGSSSL | ||||||
Compositional bias | 626-662 | Basic and acidic residues | ||||
Sequence: ESKNGKKKKNFNMSESSDDETSNSRKRRKRESSESQS | ||||||
Compositional bias | 681-696 | Polar residues | ||||
Sequence: KSGQPPSTSREWNQSS | ||||||
Compositional bias | 712-740 | Polar residues | ||||
Sequence: SRVPASSSTSVSAIGKNNAAASSNSYQAP | ||||||
Alternative sequence | VSP_057964 | 739-765 | in isoform b | |||
Sequence: APKPAPVPAPTSSRPPAAPRPPSKPKK → KFYNCFHSYTPPLKVEKKIIKLLVNFC | ||||||
Compositional bias | 741-761 | Pro residues | ||||
Sequence: KPAPVPAPTSSRPPAAPRPPS | ||||||
Alternative sequence | VSP_057965 | 766-853 | in isoform b | |||
Sequence: Missing | ||||||
Compositional bias | 783-812 | Polar residues | ||||
Sequence: SPPQFFQSQPTTSATIRSPTESQPGNGEDE |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX284601 EMBL· GenBank· DDBJ | CCD68304.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284601 EMBL· GenBank· DDBJ | CCD68309.1 EMBL· GenBank· DDBJ | Genomic DNA |