Q95Y80 · BET1_CAEEL

  • Protein
    Bromodomain-containing protein bet-1
  • Gene
    bet-1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Required for the establishment and maintenance of stable cell fate in several lineages including V5.pa, T, Z1/Z4 and QR lineages probably by repressing the expression of cell fate determinants (PubMed:20181741, PubMed:24346701).
Required to maintain non-distal tip cell (DTC) fate of somatic gonadal cells through the htz-1-mediated repression of transcription factor ceh-22. Regulates the subnuclear localization of histone variant htz-1 in somatic gonadal cells (PubMed:24346701).
Plays a role in the attenuation of the let-60/ras pathway, probably by preventing expression of activators of the pathway (PubMed:24285704, PubMed:24349540).
Involved in adult locomotion. Acts together with the sumoylation pathway to prevent muscle myosin depletion in aging adults probably by preventing myoblast growth factor receptor egl-15 overexpression (PubMed:24285704).
May play a role in vulva development (PubMed:24349540).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentchromatin
Cellular Componentchromosome
Cellular Componentmetaphase plate
Cellular Componentnucleus
Molecular Functionlysine-acetylated histone binding
Molecular FunctionSUMO binding
Biological Processcell fate specification
Biological Processchromatin remodeling
Biological Processmulticellular organismal locomotion
Biological Processmuscle cell cellular homeostasis
Biological Processnegative regulation of cell fate specification
Biological Processnegative regulation of DNA-templated transcription
Biological Processneuroblast fate specification
Biological Processprotein localization to chromatin
Biological Processregulation of DNA-templated transcription
Biological Processregulation of protein stability
Biological Processregulation of transcription by RNA polymerase II
Biological Processregulation of vulval development

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bromodomain-containing protein bet-1

Gene names

    • Name
      bet-1
    • ORF names
      Y119C1B.8

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    Q95Y80
  • Secondary accessions
    • H2KZI7

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Chromosome
Note: Localizes to granular structures in interphase and co-localizes with chromosomes during metaphase.

Keywords

Phenotypes & Variants

Disruption phenotype

Abnormal cell fate determination of several lineages. T.p daughter cells adopt a hypodermal cell fate instead of a neuronal cell fate resulting in the loss of the phasmid socket cell. Generation of ectopic PDE, AVM and PVM neurons, and distal tip cell (DTC) (PubMed:20181741, PubMed:24346701).
In addition, ectopic egl-17 expression in multiple vulva precursor cells and ectopic che-22 expression in extra distal tip cells (PubMed:24346701, PubMed:24349540).
Moderate increase in mpk-1 phosphorylation (PubMed:24349540).
RNAi-mediated knockdown further suppresses the age-dependent paralysis phenotype of the spop-1 gk630214 or dr95 mutants (PubMed:34593637).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis113In os118; 50 percent of animals lack phasmid socket cell and have an ectopic distal tip cell; when associated with K-585.
Mutagenesis252Loss of interaction with smo-1 and ubc-9.
Mutagenesis253No effect on the interaction with smo-1 and ubc-9.
Mutagenesis264No effect on the interaction with smo-1; when associated with R-276 and R-277.
Mutagenesis276-277No effect on the interaction with smo-1; when associated with R-264.
Mutagenesis304No effect on the interaction with smo-1; when associated with R-313 and R-315.
Mutagenesis313No effect on the interaction with smo-1; when associated with R-304 and R-315.
Mutagenesis315No effect on the interaction with smo-1; when associated with R-304 and R-313.
Mutagenesis585In os118; 50 percent of animals lack phasmid socket cell and have an ectopic distal tip cell; when associated with I-113.

PTM/Processing

Features

Showing features for chain, cross-link.

TypeIDPosition(s)Description
ChainPRO_00004346031-853Bromodomain-containing protein bet-1
Cross-link252Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Keywords

Proteomic databases

Expression

Tissue specificity

Expressed in T-cells, Q-cells, V5-cells and their descendants such as somatic gonad and syncytium.

Gene expression databases

Interaction

Subunit

Interacts with acetylated histone H4 (PubMed:20181741).
Interacts (via BROMO domain 2) with smo-1 and ubc-9 (PubMed:24349540).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q95Y80siah-1 Q965X62EBI-311872, EBI-311877

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for compositional bias, region, domain, coiled coil.

TypeIDPosition(s)Description
Compositional bias1-17Polar residues
Region1-22Disordered
Domain56-128Bromo 1
Compositional bias141-167Basic and acidic residues
Region141-245Disordered
Compositional bias189-208Polar residues
Compositional bias218-245Basic and acidic residues
Domain277-349Bromo 2
Region369-418Disordered
Compositional bias393-414Basic and acidic residues
Coiled coil419-458
Domain516-601NET
Region594-814Disordered
Compositional bias601-625Polar residues
Compositional bias626-662Basic and acidic residues
Compositional bias681-696Polar residues
Compositional bias712-740Polar residues
Compositional bias741-761Pro residues
Compositional bias783-812Polar residues
Region819-838Disordered

Domain

The BROMO domain 2 is essential for the interaction with smo-1 and E2 enzyme ubc-9.

Sequence similarities

Belongs to the BET family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q95Y80-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    853
  • Mass (Da)
    94,086
  • Last updated
    2012-01-25 v3
  • Checksum
    7B4C4A18226A35A5
MSEGSGDQSQQRPWASPRQQPIKGIVQPRVLPPFGKPTRHTNKLDYIMTTVLKEAGKHKHVWPFQKPVDAVALCIPLYHERVARPMDLKTIENRLKSTYYTCAQECIDDIETVFQNCYTFNGKEDDVTIMAQNVHEVIKKSLEQAPREEHDMDVYWGKNKKKPAKSDGGSKSSSSKKNDARGPSEAPSEAGSEVSSVTTASAAAPTVSESASVAAKPERKVAGKKTGKRKAESEDDEKPEPLRAKREVAVVKKEVHQPLLPSMKPCLKLLNDFSTKKYQEFAWPFNEPVDAEQLGLHDYHKIIKEPMDLKSMKAKMESGAYKEPSDFEHDVRLMLRNCFLYNPVGDPVHSFGLRFQEVFDRRWAELGDSSSRASSVAPQSAPIAPTPKVAKSSAPKEPKESRKEHKKETTFEASGAKSEDLMQINNALSMIREREEKLKAELAAAQAIKDKLTSVKNRREDNPNEPFPEKLINETRALCTTQVGQNASSSSASSAALRNGRSKKAASARLYGYEFDSDDEDNKMALTYEEKRNLSNLINNLPNNQLNTIISIIQRRERSALMQQQLDDSEVELDFESLGDMCLREMGAFIKTIPTLNGNGDDEKPKTSSNPTSSGATGSKGSSSLESKNGKKKKNFNMSESSDDETSNSRKRRKRESSESQSSSSSDDDSDDEDRPSIPRKSGQPPSTSREWNQSSAPPPRMGGMGGQPPMSRVPASSSTSVSAIGKNNAAASSNSYQAPKPAPVPAPTSSRPPAAPRPPSKPKKTGGASILDTLLPDTFGASPPQFFQSQPTTSATIRSPTESQPGNGEDEQTRIQRMRMEAKRARQKEDEGSVSLSNQMEMMAAFEFDNTY

Q95Y80-2

  • Name
    b
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 739-765: APKPAPVPAPTSSRPPAAPRPPSKPKK → KFYNCFHSYTPPLKVEKKIIKLLVNFC
    • 766-853: Missing

Computationally mapped potential isoform sequences

There are 6 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A5S9MPT0A0A5S9MPT0_CAEELbet-1857
A0A5S9MQE5A0A5S9MQE5_CAEELbet-1854
A0A5S9MM65A0A5S9MM65_CAEELbet-1852
A0A5S9MMD4A0A5S9MMD4_CAEELbet-1852
A0A5S9MMP9A0A5S9MMP9_CAEELbet-1855
A0A5S9MN31A0A5S9MN31_CAEELbet-1855

Features

Showing features for compositional bias, alternative sequence.

TypeIDPosition(s)Description
Compositional bias1-17Polar residues
Compositional bias141-167Basic and acidic residues
Compositional bias189-208Polar residues
Compositional bias218-245Basic and acidic residues
Compositional bias393-414Basic and acidic residues
Compositional bias601-625Polar residues
Compositional bias626-662Basic and acidic residues
Compositional bias681-696Polar residues
Compositional bias712-740Polar residues
Alternative sequenceVSP_057964739-765in isoform b
Compositional bias741-761Pro residues
Alternative sequenceVSP_057965766-853in isoform b
Compositional bias783-812Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BX284601
EMBL· GenBank· DDBJ
CCD68304.1
EMBL· GenBank· DDBJ
Genomic DNA
BX284601
EMBL· GenBank· DDBJ
CCD68309.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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