Q95XP9 · ZWILC_CAEEL
- ProteinProtein zwilch homolog
- Genezwl-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids630 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis (PubMed:18765790).
Required for chromosome segregation, the assembly of the dynein-dynactin and mdf-1-mdf-2 complexes onto kinetochores and spindle pole separation (PubMed:18765790).
Its function related to the spindle assembly machinery and kinetochore-microtubule attachments likely depends on its association in the mitotic RZZ complex (PubMed:18765790).
The RZZ complex recruits the spindly-like protein spdl-1 to kinetochores (PubMed:18765790).
To prevent irregular chromosome segregation, the complex also inhibits the attachment of the kinetochore-associated NDC80 complex to microtubules (PubMed:24231804).
The recruitment of spdl-1 to kinetochores relieves this inhibition (PubMed:24231804).
Required for embryonic development (PubMed:18765790, PubMed:19109417).
Required for chromosome segregation, the assembly of the dynein-dynactin and mdf-1-mdf-2 complexes onto kinetochores and spindle pole separation (PubMed:18765790).
Its function related to the spindle assembly machinery and kinetochore-microtubule attachments likely depends on its association in the mitotic RZZ complex (PubMed:18765790).
The RZZ complex recruits the spindly-like protein spdl-1 to kinetochores (PubMed:18765790).
To prevent irregular chromosome segregation, the complex also inhibits the attachment of the kinetochore-associated NDC80 complex to microtubules (PubMed:24231804).
The recruitment of spdl-1 to kinetochores relieves this inhibition (PubMed:24231804).
Required for embryonic development (PubMed:18765790, PubMed:19109417).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cell cortex | |
Cellular Component | kinetochore | |
Cellular Component | RZZ complex | |
Cellular Component | spindle | |
Biological Process | cell division | |
Biological Process | meiotic cell cycle | |
Biological Process | mitotic spindle assembly checkpoint signaling | |
Biological Process | positive regulation of mitotic spindle pole body separation | |
Biological Process | positive regulation of protein localization to kinetochore | |
Biological Process | protein localization to kinetochore | |
Biological Process | protein localization to kinetochore involved in kinetochore assembly | |
Biological Process | regulation of attachment of spindle microtubules to kinetochore |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameProtein zwilch homolog
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ95XP9
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localizes to the kinetochore during nuclear envelope breakdown and remains there until the metaphase-anaphase transition (PubMed:18765790).
Localizes to kinetochores, spindle-associated rod-shaped structures and near the cell cortex in a knl-1-dependent manner in anaphase (PubMed:18765790, PubMed:20729837).
Localizes to kinetochores, spindle-associated rod-shaped structures and near the cell cortex in a knl-1-dependent manner in anaphase (PubMed:18765790, PubMed:20729837).
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
RNAi-mediated knockdown results in embryonic lethality (PubMed:18765790, PubMed:19109417).
RNAi-mediated knockdown results in defective chromosome segregation, premature spindle pole separation and incorrectly attached kinetochores (PubMed:18765790).
In addition, knl-1 localization to the spindle-associated rod-shaped structures but not to kinetochores is lost during anaphase of meiosis I (PubMed:20729837).
RNAi-mediated knockdown also results in abolished targeting of the spindly-like protein spdl-1 to the kinetochore (PubMed:18765790).
RNAi-mediated knockdown results in defective chromosome segregation, premature spindle pole separation and incorrectly attached kinetochores (PubMed:18765790).
In addition, knl-1 localization to the spindle-associated rod-shaped structures but not to kinetochores is lost during anaphase of meiosis I (PubMed:20729837).
RNAi-mediated knockdown also results in abolished targeting of the spindly-like protein spdl-1 to the kinetochore (PubMed:18765790).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000314807 | 1-630 | Protein zwilch homolog | |||
Sequence: MPLTIEQLKQYNEKMAAKGEEDDAPAPVLLLDKYRVRVLPLTSIPFVMNHSSVSQLSLSSEDVLVVDFPSKGTGSGAGKAEKKMIFKKKIEPMEDSFEDKENVNEGGPLMTSFLTLEKLRKGMVGDVEIIGYECETSFFDANPIPLSDGVSLQRYIRLNCSEHFSPSISTLPVWISTTSSKFPSICWLAAGRTNRNVQFAAATRVLGHFNNENSERVVKQLNQACGTSQLNKYRAVYEEIRKIATENRPAPGEVTIDVRWSTKSTLVLLEHPDNAADCTIKIDLGWGDKRFFVDDEIFEQLFFVLNLADVLANPDNEVIFPMAPPANFDDLVQEMDALVEASSREDNVFVSNENFRGGDITDKVWNIVRKCNDVKQVTLLFRNFLQALAYGKIKSHAQERNKSHLASLIRISKSSEFKIPVLERLSTIDMMMEIGVESLRRRVIDIFSSQLLYPSDELEFILQTCENDLPAGNGAMNSAAISLLPITMALATANRIYELLNEKDHVILPDLTRRILQKYTASMIEKSRRGETETEYTFETTLPLLRMYKEGFMSKRPCIWTCENSNTVGANVQARVLTSLELQPSLEHINRLVNDSRPVWTATEEKPAMIKDLNADYTVVHTIFSYLPKM |
Proteomic databases
Expression
Gene expression databases
Structure
Sequence & Isoforms
- Sequence statusComplete
This entry describes 4 isoforms produced by Alternative splicing.
Q95XP9-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Namea
- Length630
- Mass (Da)71,096
- Last updated2001-12-01 v1
- ChecksumA5E4373C5E262928
Q95XP9-2
- Nameb
- Differences from canonical
- 354-356: NFR → K
Q95XP9-3
- Namec
- Differences from canonical
- 1-321: Missing
Q95XP9-4
- Named
Features
Showing features for alternative sequence.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FO081209 EMBL· GenBank· DDBJ | CCD69901.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
FO081209 EMBL· GenBank· DDBJ | CCE67248.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
FO081209 EMBL· GenBank· DDBJ | CDR32818.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
FO081209 EMBL· GenBank· DDBJ | CDR32819.1 EMBL· GenBank· DDBJ | Genomic DNA |