Q95XG8 · NRAMC_CAEEL
- ProteinNRAMP-like transporter smf-3
- Genesmf-3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids560 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Probable divalent metal ion transporter which regulates the uptake of several heavy metals such as Mn2+, Al3+ and iron (PubMed:19785996, PubMed:19924247, PubMed:22194696, PubMed:23106139).
Plays a role in modulating Al3+-induced dopamine (DA) neuron degeneration through the intracellular sequestration of Al3+ (PubMed:23106139).
Plays a role in modulating Al3+-induced dopamine (DA) neuron degeneration through the intracellular sequestration of Al3+ (PubMed:23106139).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | apical plasma membrane | |
Cellular Component | endosome membrane | |
Cellular Component | organelle membrane | |
Cellular Component | plasma membrane | |
Molecular Function | cadmium ion transmembrane transporter activity | |
Molecular Function | iron ion transmembrane transporter activity | |
Molecular Function | manganese ion transmembrane transporter activity | |
Molecular Function | transition metal ion transmembrane transporter activity | |
Biological Process | defense response to Gram-negative bacterium | |
Biological Process | intracellular iron ion homeostasis | |
Biological Process | iron ion transmembrane transport | |
Biological Process | manganese ion homeostasis | |
Biological Process | manganese ion transport | |
Biological Process | response to manganese ion | |
Biological Process | response to metal ion | |
Biological Process | response to oxidative stress | |
Biological Process | sequestering of iron ion | |
Biological Process | transition metal ion transport |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameNRAMP-like transporter smf-3
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ95XG8
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Apical cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-43 | Cytoplasmic | ||||
Sequence: MEGEMKCPIEEIREKPEMRKAQQTYEVQVEVEDTPDTTFSWRK | ||||||
Transmembrane | 44-64 | Helical | ||||
Sequence: LWAFTGPGFLMSIAYLDPGNI | ||||||
Topological domain | 65-71 | Extracellular | ||||
Sequence: ESDLQAG | ||||||
Transmembrane | 72-92 | Helical | ||||
Sequence: AISYFKLIWVLLVAHIMGLLL | ||||||
Topological domain | 93-120 | Cytoplasmic | ||||
Sequence: QRLAARLGVVSGKHMAEIAFSYYPKIPR | ||||||
Transmembrane | 121-141 | Helical | ||||
Sequence: LVLWMLVESAIVGSDMQEVIG | ||||||
Topological domain | 142-152 | Extracellular | ||||
Sequence: TAISFYLLSNG | ||||||
Transmembrane | 153-173 | Helical | ||||
Sequence: VIPLWAGVLITICDTFTFLFL | ||||||
Topological domain | 174-182 | Cytoplasmic | ||||
Sequence: EKYGVRKFE | ||||||
Transmembrane | 183-203 | Helical | ||||
Sequence: AFFCFLITCMAITFGYEFGVS | ||||||
Topological domain | 204-229 | Extracellular | ||||
Sequence: APDAGKMFSGMFVPWCNGCDNNMVMQ | ||||||
Transmembrane | 230-250 | Helical | ||||
Sequence: GVAIIGAVIMPHNFYLHSALV | ||||||
Topological domain | 251-268 | Cytoplasmic | ||||
Sequence: KSRRVDRRRAEKVTEANK | ||||||
Transmembrane | 269-289 | Helical | ||||
Sequence: YFFIESAFALFVSFIINTLVI | ||||||
Topological domain | 290-339 | Extracellular | ||||
Sequence: SVFAQGMYGKTNQDIRDTCYNNTHNGMPDFYKVEFPANNDAAQSDIYHAG | ||||||
Transmembrane | 340-360 | Helical | ||||
Sequence: IFLGCTFGIFALYVWAVGILA | ||||||
Topological domain | 361-390 | Cytoplasmic | ||||
Sequence: AGQSSTMTGTYAGQFAMEGFIQIKLPQWKR | ||||||
Transmembrane | 391-411 | Helical | ||||
Sequence: ILITRSLAILPTLAVVIFSGG | ||||||
Topological domain | 412-420 | Extracellular | ||||
Sequence: IDNISSLND | ||||||
Transmembrane | 421-441 | Helical | ||||
Sequence: FLNCLQLIQLPFALIPVLTFV | ||||||
Topological domain | 442-458 | Cytoplasmic | ||||
Sequence: SDRNIMHEYKLASVSKV | ||||||
Transmembrane | 459-479 | Helical | ||||
Sequence: VSIVISLIILFINFYFLYSWI | ||||||
Topological domain | 480-486 | Extracellular | ||||
Sequence: GSTFGYN | ||||||
Transmembrane | 487-507 | Helical | ||||
Sequence: AVSIPITIFCAIFYIIFIAYL | ||||||
Topological domain | 508-560 | Cytoplasmic | ||||
Sequence: TYYCLVAMEFISPIQTKWLAEPIYHDFDAPWLEDSENPSTKNTISDDELSMRY |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Increased survival rate and reduced body levels of iron in response to increasing Mn2+ levels (PubMed:19924247).
Reduced survival rate in response to high Mn2+ levels or to infection mediated by pathogenic bacterium S.aureus (PubMed:19785996).
Reduced CEP neuron death and higher Al3+ accumulation in the body in response to high Al3+ levels (PubMed:23106139).
Reduced ferritin ftn-1 mRNA levels and Mn2+ and iron body levels (PubMed:22194696).
Increased sfm-1, sfm-2 and sfm-3 mRNA levels (PubMed:19924247).
Reduced survival rate in response to high Mn2+ levels or to infection mediated by pathogenic bacterium S.aureus (PubMed:19785996).
Reduced CEP neuron death and higher Al3+ accumulation in the body in response to high Al3+ levels (PubMed:23106139).
Reduced ferritin ftn-1 mRNA levels and Mn2+ and iron body levels (PubMed:22194696).
Increased sfm-1, sfm-2 and sfm-3 mRNA levels (PubMed:19924247).
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000437466 | 1-560 | NRAMP-like transporter smf-3 | |||
Sequence: MEGEMKCPIEEIREKPEMRKAQQTYEVQVEVEDTPDTTFSWRKLWAFTGPGFLMSIAYLDPGNIESDLQAGAISYFKLIWVLLVAHIMGLLLQRLAARLGVVSGKHMAEIAFSYYPKIPRLVLWMLVESAIVGSDMQEVIGTAISFYLLSNGVIPLWAGVLITICDTFTFLFLEKYGVRKFEAFFCFLITCMAITFGYEFGVSAPDAGKMFSGMFVPWCNGCDNNMVMQGVAIIGAVIMPHNFYLHSALVKSRRVDRRRAEKVTEANKYFFIESAFALFVSFIINTLVISVFAQGMYGKTNQDIRDTCYNNTHNGMPDFYKVEFPANNDAAQSDIYHAGIFLGCTFGIFALYVWAVGILAAGQSSTMTGTYAGQFAMEGFIQIKLPQWKRILITRSLAILPTLAVVIFSGGIDNISSLNDFLNCLQLIQLPFALIPVLTFVSDRNIMHEYKLASVSKVVSIVISLIILFINFYFLYSWIGSTFGYNAVSIPITIFCAIFYIIFIAYLTYYCLVAMEFISPIQTKWLAEPIYHDFDAPWLEDSENPSTKNTISDDELSMRY | ||||||
Glycosylation | 310 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 414 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in dopaminergic neurons (at protein level) (PubMed:23106139).
Expressed in intestine with a weaker expression in the most proximal and distal regions (PubMed:19785996, PubMed:19924247).
Weakly expressed in the hyp1-6, hyp7 and hyp8-12 hypodermis and in head and tail neurons (PubMed:19924247).
Expressed in intestine with a weaker expression in the most proximal and distal regions (PubMed:19785996, PubMed:19924247).
Weakly expressed in the hyp1-6, hyp7 and hyp8-12 hypodermis and in head and tail neurons (PubMed:19924247).
Induction
Induced by pathogenic bacterium S.aureus (PubMed:19785996).
Repressed by high levels of Mn2+ (PubMed:19924247).
Repressed by high levels of Al3+ (PubMed:23106139).
Induced by low iron levels (PubMed:22194696).
Repressed by high levels of Mn2+ (PubMed:19924247).
Repressed by high levels of Al3+ (PubMed:23106139).
Induced by low iron levels (PubMed:22194696).
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length560
- Mass (Da)63,186
- Last updated2012-01-25 v3
- ChecksumBCFF0BC8B3055ABC
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX284604 EMBL· GenBank· DDBJ | CCD74141.1 EMBL· GenBank· DDBJ | Genomic DNA |