Q95V25 · SLO1_CAEEL
- ProteinCalcium-activated potassium channel slo-1
- Geneslo-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1140 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Potassium channel activated by both membrane depolarization or increase in cytosolic Ca2+ that mediates export of K+ (PubMed:11738032).
Its activation dampens the excitatory events that elevate the cytosolic Ca2+ concentration and/or depolarize the cell membrane (PubMed:11738032).
It therefore contributes to repolarization of the membrane potential (PubMed:11738032).
Essential for the regulation of neurotransmitter release at synapses (PubMed:11738032, PubMed:28168949).
Its activation dampens the excitatory events that elevate the cytosolic Ca2+ concentration and/or depolarize the cell membrane (PubMed:11738032).
It therefore contributes to repolarization of the membrane potential (PubMed:11738032).
Essential for the regulation of neurotransmitter release at synapses (PubMed:11738032, PubMed:28168949).
Miscellaneous
Its activity is activated by ethanol, leading to the inhibition of neuronal activity.
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | M band | |
Cellular Component | monoatomic ion channel complex | |
Cellular Component | postsynaptic membrane | |
Cellular Component | striated muscle dense body | |
Cellular Component | synapse | |
Molecular Function | calcium-activated potassium channel activity | |
Molecular Function | large conductance calcium-activated potassium channel activity | |
Molecular Function | metal ion binding | |
Molecular Function | voltage-gated potassium channel activity | |
Biological Process | behavioral response to ethanol | |
Biological Process | locomotion | |
Biological Process | modulation of chemical synaptic transmission | |
Biological Process | nematode pharyngeal pumping | |
Biological Process | potassium ion transmembrane transport | |
Biological Process | potassium ion transport | |
Biological Process | regulation of neurotransmitter secretion | |
Biological Process | response to xenobiotic stimulus | |
Biological Process | sarcomere organization |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameCalcium-activated potassium channel slo-1
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ95V25
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane, intramembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-44 | Extracellular | ||||
Sequence: MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERK | ||||||
Transmembrane | 45-65 | Helical; Name=Segment S0 | ||||
Sequence: YWCFLLSSITTFCASMILVVI | ||||||
Topological domain | 66-139 | Cytoplasmic | ||||
Sequence: WRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGR | ||||||
Transmembrane | 140-161 | Helical; Name=Segment S1 | ||||
Sequence: FLVLLVFILSIGSLIIYFYDAS | ||||||
Topological domain | 162-178 | Extracellular | ||||
Sequence: FQNFQVETCIPWQDSPS | ||||||
Transmembrane | 179-199 | Helical; Name=Segment S2 | ||||
Sequence: QQIDLGFNIFFLVYFFIRFIA | ||||||
Topological domain | 200-203 | Cytoplasmic | ||||
Sequence: ASDK | ||||||
Transmembrane | 204-224 | Helical; Name=Segment S3 | ||||
Sequence: VWFLLEMYSWIDFFTIPPSFV | ||||||
Topological domain | 225-228 | Extracellular | ||||
Sequence: AIYL | ||||||
Transmembrane | 229-249 | Helical; Voltage-sensor; Name=Segment S4 | ||||
Sequence: QRNWLGFRFLRALRLMTVPDI | ||||||
Topological domain | 250-264 | Cytoplasmic | ||||
Sequence: LQYLNILKTSSSIRL | ||||||
Transmembrane | 265-285 | Helical; Name=Segment S5 | ||||
Sequence: TQLVTIFVAVCLTGAGLVHLL | ||||||
Topological domain | 286-299 | Extracellular | ||||
Sequence: ENSGDFFKGFINPH | ||||||
Intramembrane | 300-322 | Pore-forming; Name=P region | ||||
Sequence: RITYADSVYFVLVTMSTVGYGDI | ||||||
Topological domain | 323-331 | Extracellular | ||||
Sequence: YCTTLCGRL | ||||||
Transmembrane | 332-352 | Helical; Name=Segment S6 | ||||
Sequence: FMIFFILFGLAMFASYVPEIA | ||||||
Topological domain | 353-1140 | Cytoplasmic | ||||
Sequence: DLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTSDTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 289 | In md1715; induces prolonged synaptic release. | ||||
Sequence: G → E | ||||||
Mutagenesis | 350 | In ky399gf; gain of function. Insensitive to the acetylcholinesterase inhibitor aldicarb, and locomotory speed defects. In an erg-28 mutant background, these defects are suppressed. | ||||
Sequence: E → K |
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000054140 | 1-1140 | Calcium-activated potassium channel slo-1 | |||
Sequence: MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTSDTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF |
Post-translational modification
Phosphorylated.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in synaptic regions of the nervous system including in both the nerve ring and nerve cords, as well as in the body-wall and vulval muscle.
Gene expression databases
Structure
Family & Domains
Features
Showing features for motif, domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 316-319 | Selectivity for potassium | ||||
Sequence: TVGY | ||||||
Domain | 371-514 | RCK N-terminal 1 | ||||
Sequence: KKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVIC | ||||||
Region | 520-540 | Segment S7 | ||||
Sequence: LGFIAQSCLAPGFSTMMANLF | ||||||
Region | 578-598 | Segment S8 | ||||
Sequence: MTFPEAVDLLFNRLGLLLLAI | ||||||
Region | 797-817 | Segment S9 | ||||
Sequence: VLNGHVVVCLFADQDSPLIGL | ||||||
Domain | 799-953 | RCK N-terminal 2 | ||||
Sequence: NGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPM | ||||||
Motif | 955-977 | Calcium bowl | ||||
Sequence: TELVNDSNVQFLDQDDDDDPDTE | ||||||
Region | 984-1004 | Segment S10 | ||||
Sequence: FACGTAFAISVLDSLMSTTYF |
Domain
The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor.
The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium (By similarity).
The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel. It includes binding sites for Ca2+ and Mg2+ (By similarity).
The calcium bowl constitutes one of the Ca2+ sensors and probably acts as a Ca2+-binding site.
Sequence similarities
Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. Slo sub-subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
Q95V25-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Namea
- Length1,140
- Mass (Da)129,530
- Last updated2004-04-13 v2
- Checksum15670CB2CEA0F412
Q95V25-2
- Nameb
- Differences from canonical
- 713-735: SSTSDTHLNTKSLRFAYEIKKLM → R
Q95V25-3
- Namec
Computationally mapped potential isoform sequences
There are 10 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
H7BRC9 | H7BRC9_CAEEL | slo-1 | 1160 | ||
H7BRM4 | H7BRM4_CAEEL | slo-1 | 1153 | ||
H7BRM5 | H7BRM5_CAEEL | slo-1 | 1118 | ||
H7BRM7 | H7BRM7_CAEEL | slo-1 | 1131 | ||
H8ESD6 | H8ESD6_CAEEL | slo-1 | 1146 | ||
H8ESD8 | H8ESD8_CAEEL | slo-1 | 1138 | ||
H8ESE2 | H8ESE2_CAEEL | slo-1 | 1160 | ||
H8ESE3 | H8ESE3_CAEEL | slo-1 | 1153 | ||
H8ESE4 | H8ESE4_CAEEL | slo-1 | 1138 | ||
H8ESE5 | H8ESE5_CAEEL | slo-1 | 1140 |
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_009996 | 549-585 | in isoform c | |||
Sequence: QTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVD → PHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAAN | ||||||
Alternative sequence | VSP_009997 | 678 | in isoform c | |||
Sequence: R → RDYSDFDALFYQND | ||||||
Alternative sequence | VSP_009998 | 713-735 | in isoform b and isoform c | |||
Sequence: SSTSDTHLNTKSLRFAYEIKKLM → R |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF431891 EMBL· GenBank· DDBJ | AAL28102.1 EMBL· GenBank· DDBJ | mRNA | ||
AF431892 EMBL· GenBank· DDBJ | AAL28103.1 EMBL· GenBank· DDBJ | mRNA | ||
AF431893 EMBL· GenBank· DDBJ | AAL28104.1 EMBL· GenBank· DDBJ | mRNA | ||
AL021497 EMBL· GenBank· DDBJ | CAB54459.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL021497 EMBL· GenBank· DDBJ | CAD27617.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL021497 EMBL· GenBank· DDBJ | CAD27618.1 EMBL· GenBank· DDBJ | Genomic DNA |