Q95V25 · SLO1_CAEEL

  • Protein
    Calcium-activated potassium channel slo-1
  • Gene
    slo-1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Potassium channel activated by both membrane depolarization or increase in cytosolic Ca2+ that mediates export of K+ (PubMed:11738032).
Its activation dampens the excitatory events that elevate the cytosolic Ca2+ concentration and/or depolarize the cell membrane (PubMed:11738032).
It therefore contributes to repolarization of the membrane potential (PubMed:11738032).
Essential for the regulation of neurotransmitter release at synapses (PubMed:11738032, PubMed:28168949).

Miscellaneous

Its activity is activated by ethanol, leading to the inhibition of neuronal activity.

Features

Showing features for binding site.

111401002003004005006007008009001,0001,100
TypeIDPosition(s)Description
Binding site964Ca2+ (UniProtKB | ChEBI)
Binding site967Ca2+ (UniProtKB | ChEBI)
Binding site970Ca2+ (UniProtKB | ChEBI)
Binding site972Ca2+ (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular ComponentM band
Cellular Componentmonoatomic ion channel complex
Cellular Componentpostsynaptic membrane
Cellular Componentstriated muscle dense body
Cellular Componentsynapse
Molecular Functioncalcium-activated potassium channel activity
Molecular Functionlarge conductance calcium-activated potassium channel activity
Molecular Functionmetal ion binding
Molecular Functionvoltage-gated potassium channel activity
Biological Processbehavioral response to ethanol
Biological Processlocomotion
Biological Processmodulation of chemical synaptic transmission
Biological Processnematode pharyngeal pumping
Biological Processpotassium ion transmembrane transport
Biological Processpotassium ion transport
Biological Processregulation of neurotransmitter secretion
Biological Processresponse to xenobiotic stimulus
Biological Processsarcomere organization

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Calcium-activated potassium channel slo-1
  • Alternative names
    • BK channel
    • Maxi K channel (MaxiK)
    • Slo homolog
    • Slowpoke protein 1

Gene names

    • Name
      slo-1
    • ORF names
      Y51A2D.19

Organism names

  • Taxonomic identifier
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    Q95V25
  • Secondary accessions
    • Q95V26
    • Q95V27
    • Q9U268

Proteomes

Organism-specific databases

Subcellular Location

Features

Showing features for topological domain, transmembrane, intramembrane.

TypeIDPosition(s)Description
Topological domain1-44Extracellular
Transmembrane45-65Helical; Name=Segment S0
Topological domain66-139Cytoplasmic
Transmembrane140-161Helical; Name=Segment S1
Topological domain162-178Extracellular
Transmembrane179-199Helical; Name=Segment S2
Topological domain200-203Cytoplasmic
Transmembrane204-224Helical; Name=Segment S3
Topological domain225-228Extracellular
Transmembrane229-249Helical; Voltage-sensor; Name=Segment S4
Topological domain250-264Cytoplasmic
Transmembrane265-285Helical; Name=Segment S5
Topological domain286-299Extracellular
Intramembrane300-322Pore-forming; Name=P region
Topological domain323-331Extracellular
Transmembrane332-352Helical; Name=Segment S6
Topological domain353-1140Cytoplasmic

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis289In md1715; induces prolonged synaptic release.
Mutagenesis350In ky399gf; gain of function. Insensitive to the acetylcholinesterase inhibitor aldicarb, and locomotory speed defects. In an erg-28 mutant background, these defects are suppressed.

Chemistry

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000541401-1140Calcium-activated potassium channel slo-1

Post-translational modification

Phosphorylated.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in synaptic regions of the nervous system including in both the nerve ring and nerve cords, as well as in the body-wall and vulval muscle.

Gene expression databases

Interaction

Subunit

Homotetramer; which constitutes the calcium-activated potassium channel.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for motif, domain, region.

TypeIDPosition(s)Description
Motif316-319Selectivity for potassium
Domain371-514RCK N-terminal 1
Region520-540Segment S7
Region578-598Segment S8
Region797-817Segment S9
Domain799-953RCK N-terminal 2
Motif955-977Calcium bowl
Region984-1004Segment S10

Domain

The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor.
The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium (By similarity).
The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel. It includes binding sites for Ca2+ and Mg2+ (By similarity).
The calcium bowl constitutes one of the Ca2+ sensors and probably acts as a Ca2+-binding site.

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete

This entry describes 3 isoforms produced by Alternative splicing.

Q95V25-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    1,140
  • Mass (Da)
    129,530
  • Last updated
    2004-04-13 v2
  • Checksum
    15670CB2CEA0F412
MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTSDTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Q95V25-2

  • Name
    b
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 713-735: SSTSDTHLNTKSLRFAYEIKKLM → R

Q95V25-3

  • Name
    c
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 549-585: QTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVD → PHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAAN
    • 678-678: R → RDYSDFDALFYQND
    • 713-735: SSTSDTHLNTKSLRFAYEIKKLM → R

Computationally mapped potential isoform sequences

There are 10 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
H7BRC9H7BRC9_CAEELslo-11160
H7BRM4H7BRM4_CAEELslo-11153
H7BRM5H7BRM5_CAEELslo-11118
H7BRM7H7BRM7_CAEELslo-11131
H8ESD6H8ESD6_CAEELslo-11146
H8ESD8H8ESD8_CAEELslo-11138
H8ESE2H8ESE2_CAEELslo-11160
H8ESE3H8ESE3_CAEELslo-11153
H8ESE4H8ESE4_CAEELslo-11138
H8ESE5H8ESE5_CAEELslo-11140

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_009996549-585in isoform c
Alternative sequenceVSP_009997678in isoform c
Alternative sequenceVSP_009998713-735in isoform b and isoform c

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF431891
EMBL· GenBank· DDBJ
AAL28102.1
EMBL· GenBank· DDBJ
mRNA
AF431892
EMBL· GenBank· DDBJ
AAL28103.1
EMBL· GenBank· DDBJ
mRNA
AF431893
EMBL· GenBank· DDBJ
AAL28104.1
EMBL· GenBank· DDBJ
mRNA
AL021497
EMBL· GenBank· DDBJ
CAB54459.2
EMBL· GenBank· DDBJ
Genomic DNA
AL021497
EMBL· GenBank· DDBJ
CAD27617.1
EMBL· GenBank· DDBJ
Genomic DNA
AL021497
EMBL· GenBank· DDBJ
CAD27618.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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