Q95QD7 · LIN54_CAEEL
- ProteinProtein lin-54
- Genelin-54
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids435 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Synthetic multivulva class B (synMuvB) protein. SynMuvB proteins are required to repress the induction of vulval development by Ras signaling and probably act by forming the multiprotein DRM complex that repress transcription.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 177 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 177 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 179 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 184 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 184 | Zn2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Site | 188 | Critical for interaction with target DNA | ||||
Sequence: Y | ||||||
Binding site | 189 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 191 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 198 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 198 | Zn2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 201 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 203 | Zn2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 206 | Zn2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Site | 226 | Interaction with DNA | ||||
Sequence: R | ||||||
Binding site | 253 | Zn2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 253 | Zn2+ 5 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 255 | Zn2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 260 | Zn2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 260 | Zn2+ 6 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Site | 264 | Critical for interaction with target DNA | ||||
Sequence: Y | ||||||
Binding site | 265 | Zn2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 267 | Zn2+ 5 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 274 | Zn2+ 5 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 274 | Zn2+ 6 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 278 | Zn2+ 5 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 280 | Zn2+ 6 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 283 | Zn2+ 6 (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | autosome | |
Cellular Component | condensed nuclear chromosome | |
Cellular Component | DRM complex | |
Cellular Component | nucleus | |
Molecular Function | metal ion binding | |
Molecular Function | RNA polymerase II transcription regulatory region sequence-specific DNA binding | |
Biological Process | negative regulation of vulval development | |
Biological Process | regulation of DNA-templated transcription | |
Biological Process | regulation of transcription by RNA polymerase II |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein lin-54
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ95QD7
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localizes to condensed chromosomes during the diakinesis phase of meiosis.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Decreased protein levels of DRM complex components including lin-9 and lin-52 (PubMed:17075059).
Double knockout with the programmed cell death regulator mcd-1 results in 100% lethality during the L1 stage of larval development (PubMed:17237514).
RNAi-mediated knockdown in a ced-1 mutant background results in reduced somatic cell apoptosis (PubMed:27650246).
Double knockout with the programmed cell death regulator mcd-1 results in 100% lethality during the L1 stage of larval development (PubMed:17237514).
RNAi-mediated knockdown in a ced-1 mutant background results in reduced somatic cell apoptosis (PubMed:27650246).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000341395 | 1-435 | Protein lin-54 | |||
Sequence: MNQGEIVYQDDDDYYDESEIYDNYEEGAEFIEVNGQLVPHNPNLQAQQNRPGTSSMIQQHNRSMEVNQGLVKDEPIDTSSHRVYVPPPRPVQRKLFQPGPSTPGSSQYTVRNLSNLSGSPSMYDRQPASLPRTVQPMGLEMGNSEQRKVYIDMKDHVSHIRLKTKKKVFAPGQRKPCNCTKSQCLKLYCDCFANGEFCRDCNCKDCHNNIEYDSQRSKAIRQSLERNPNAFKPKIGIARGGITDIERLHQKGCHCKKSGCLKNYCECYEAKVPCTDRCKCKGCQNTETYRMTRYKNSGGAVSNTNALMSLTNASSTATPDSGPGSVVTDEHGDDYEDMLLSHKPKVEMDPRRFPWYYMTDEVVEAATMCMVAQAEEALNYEKVQTEDEKLINMEKLVLREFGRCLEQMITNTTELTQDLDAAPTDDIPGPSTSTS |
Proteomic databases
Expression
Developmental stage
Widely expressed. Present in most cells from the embryo through the adulthood.
Gene expression databases
Interaction
Subunit
Component of the DRM complex, at least composed of lin-9, lin-35, lin-37, lin-52, lin-53, lin-54- dpl-1 and efl-1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q95QD7 | lin-37 Q23482 | 6 | EBI-6762766, EBI-324325 |
Complex viewer
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 73-136 | Disordered | ||||
Sequence: DEPIDTSSHRVYVPPPRPVQRKLFQPGPSTPGSSQYTVRNLSNLSGSPSMYDRQPASLPRTVQP | ||||||
Compositional bias | 98-128 | Polar residues | ||||
Sequence: PGPSTPGSSQYTVRNLSNLSGSPSMYDRQPA | ||||||
Domain | 173-288 | CRC | ||||
Sequence: QRKPCNCTKSQCLKLYCDCFANGEFCRDCNCKDCHNNIEYDSQRSKAIRQSLERNPNAFKPKIGIARGGITDIERLHQKGCHCKKSGCLKNYCECYEAKVPCTDRCKCKGCQNTET | ||||||
Region | 175-188 | DNA-binding | ||||
Sequence: KPCNCTKSQCLKLY | ||||||
Region | 235-250 | Linker | ||||
Sequence: IGIARGGITDIERLHQ | ||||||
Region | 253-266 | DNA-binding | ||||
Sequence: CHCKKSGCLKNYCE | ||||||
Region | 415-435 | Disordered | ||||
Sequence: LTQDLDAAPTDDIPGPSTSTS |
Domain
The CRC domain mediates DNA-binding. It contains two CXC subdomains (joined by a flexible linker) which are both required for efficient association with target DNA. Each CXC subdomain coordinates three Zn2+ ions.
Sequence similarities
Belongs to the lin-54 family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q95QD7-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Namea
- Length435
- Mass (Da)49,109
- Last updated2001-12-01 v1
- Checksum7629A5C749D25A5D
Q95QD7-2
- Nameb
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A486WX88 | A0A486WX88_CAEEL | lin-54 | 432 | ||
A0A486WWG3 | A0A486WWG3_CAEEL | lin-54 | 432 |
Features
Showing features for alternative sequence, compositional bias.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z82274 EMBL· GenBank· DDBJ | CAB05229.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z82274 EMBL· GenBank· DDBJ | CAB05228.1 EMBL· GenBank· DDBJ | Genomic DNA |