Q95JD4 · RHBG_RABIT
- ProteinAmmonium transporter Rh type B
- GeneRHBG
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids458 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Ammonium transporter involved in the maintenance of acid-base homeostasis. Transports ammonium and its related derivative methylammonium across the basolateral plasma membrane of epithelial cells likely contributing to renal transepithelial ammonia transport and ammonia metabolism. May transport either NH4+ or NH3 ammonia species predominantly mediating an electrogenic NH4+ transport (By similarity).
May act as a CO2 channel providing for renal acid secretion (By similarity).
May act as a CO2 channel providing for renal acid secretion (By similarity).
Catalytic activity
- NH4+(in) = NH4+(out)This reaction proceeds in the forward and the backward directions.
- methylamine(out) = methylamine(in)This reaction proceeds in the forward direction.
- CO2(out) = CO2(in)This reaction proceeds in the forward direction.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | basolateral plasma membrane | |
Cellular Component | spectrin-associated cytoskeleton | |
Molecular Function | ammonium channel activity | |
Molecular Function | ankyrin binding | |
Molecular Function | carbon dioxide transmembrane transporter activity | |
Biological Process | ammonium homeostasis | |
Biological Process | ammonium transmembrane transport | |
Biological Process | transepithelial ammonium transport |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameAmmonium transporter Rh type B
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Lagomorpha > Leporidae > Oryctolagus
Accessions
- Primary accessionQ95JD4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Basolateral cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-13 | Cytoplasmic | ||||
Sequence: MAKSPRRVAGRRL | ||||||
Transmembrane | 14-34 | Helical | ||||
Sequence: LLPLLCLFFQGATAILFAIFV | ||||||
Topological domain | 35-61 | Extracellular | ||||
Sequence: RYDQQTDAALWHGGNHSNADNEFYFRY | ||||||
Transmembrane | 62-82 | Helical | ||||
Sequence: PSFQDVHAMVFVGFGFLMVFL | ||||||
Topological domain | 83-86 | Cytoplasmic | ||||
Sequence: QRYG | ||||||
Transmembrane | 87-107 | Helical | ||||
Sequence: YSSLGFTFLLGAFALQWATLV | ||||||
Topological domain | 108-124 | Extracellular | ||||
Sequence: QGFLHSFHGGHIHVGME | ||||||
Transmembrane | 125-145 | Helical | ||||
Sequence: SLINADFCAGAVLISFGAVLG | ||||||
Topological domain | 146-149 | Cytoplasmic | ||||
Sequence: KTGP | ||||||
Transmembrane | 150-170 | Helical | ||||
Sequence: AQLLLMALLEVALFGLNEFVL | ||||||
Topological domain | 171-178 | Extracellular | ||||
Sequence: LCLLGVRD | ||||||
Transmembrane | 179-201 | Helical | ||||
Sequence: AGGSMTIHTFGAYFGLVLSRVLY | ||||||
Topological domain | 202-219 | Cytoplasmic | ||||
Sequence: RPHLEKSQHRQGSVYHSD | ||||||
Transmembrane | 220-240 | Helical | ||||
Sequence: LFAMIGTIFLWIFWPSFNSAL | ||||||
Topological domain | 241-251 | Extracellular | ||||
Sequence: TSRGDGQPRTA | ||||||
Transmembrane | 252-272 | Helical | ||||
Sequence: LNTYYSLTASTLSTFALSALV | ||||||
Topological domain | 273-282 | Cytoplasmic | ||||
Sequence: GKDGRLDMVH | ||||||
Transmembrane | 283-303 | Helical | ||||
Sequence: VQNAALAGGVVVGTASEMMLT | ||||||
Topological domain | 304 | Extracellular | ||||
Sequence: P | ||||||
Transmembrane | 305-325 | Helical | ||||
Sequence: FGALAAGCLAGAISTLGYKFF | ||||||
Topological domain | 326-346 | Cytoplasmic | ||||
Sequence: TPILESKLKIQDTCGVHNLHG | ||||||
Transmembrane | 347-367 | Helical | ||||
Sequence: MPGVLGALLGALMTGLTTHEA | ||||||
Topological domain | 368-393 | Extracellular | ||||
Sequence: YGDGLQSVFPLIAEGQRSATSQAIYQ | ||||||
Transmembrane | 394-414 | Helical | ||||
Sequence: LFGLSVTLLFASAGGVLGGLL | ||||||
Topological domain | 415-458 | Cytoplasmic | ||||
Sequence: LKLPFLDAPPDSQCYEDQMCWEVPGEHGYEAQEALRVEEPDTEA |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000283604 | 1-458 | Ammonium transporter Rh type B | |||
Sequence: MAKSPRRVAGRRLLLPLLCLFFQGATAILFAIFVRYDQQTDAALWHGGNHSNADNEFYFRYPSFQDVHAMVFVGFGFLMVFLQRYGYSSLGFTFLLGAFALQWATLVQGFLHSFHGGHIHVGMESLINADFCAGAVLISFGAVLGKTGPAQLLLMALLEVALFGLNEFVLLCLLGVRDAGGSMTIHTFGAYFGLVLSRVLYRPHLEKSQHRQGSVYHSDLFAMIGTIFLWIFWPSFNSALTSRGDGQPRTALNTYYSLTASTLSTFALSALVGKDGRLDMVHVQNAALAGGVVVGTASEMMLTPFGALAAGCLAGAISTLGYKFFTPILESKLKIQDTCGVHNLHGMPGVLGALLGALMTGLTTHEAYGDGLQSVFPLIAEGQRSATSQAIYQLFGLSVTLLFASAGGVLGGLLLKLPFLDAPPDSQCYEDQMCWEVPGEHGYEAQEALRVEEPDTEA | ||||||
Glycosylation | 49 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Post-translational modification
N-glycosylated.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Interacts (via C-terminus) with ANK2 and ANK3; required for targeting to the basolateral membrane.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 416-424 | Interaction with ANK3 | ||||
Sequence: KLPFLDAPP | ||||||
Motif | 429-432 | Basolateral sorting signal | ||||
Sequence: YEDQ |
Sequence similarities
Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length458
- Mass (Da)49,425
- Last updated2001-12-01 v1
- Checksum0FC41E08EC78B357
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY013262 EMBL· GenBank· DDBJ | AAK14652.1 EMBL· GenBank· DDBJ | mRNA |