Q95165 · SCNNB_CANLF
- ProteinAmiloride-sensitive sodium channel subunit beta
- GeneSCNN1B
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids641 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
Activity regulation
Activated by WNK1, WNK2, WNK3 and WNK4.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | apical plasma membrane | |
Cellular Component | cytoplasmic vesicle membrane | |
Cellular Component | plasma membrane | |
Cellular Component | sodium channel complex | |
Molecular Function | ligand-gated sodium channel activity | |
Biological Process | multicellular organismal-level water homeostasis | |
Biological Process | sensory perception of taste | |
Biological Process | sodium ion homeostasis | |
Biological Process | sodium ion transmembrane transport |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameAmiloride-sensitive sodium channel subunit beta
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Carnivora > Caniformia > Canidae > Canis
Accessions
- Primary accessionQ95165
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Apical cell membrane ; Multi-pass membrane protein
Note: Apical membrane of epithelial cells.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-50 | Cytoplasmic | ||||
Sequence: MHLKKYLLKGLHRLQKGPGYSYKELLVWYCNNTNTHGPKRIICEGPKKKA | ||||||
Transmembrane | 51-71 | Helical; Name=1 | ||||
Sequence: MWFLITLLFTSLVCWQWGVFI | ||||||
Topological domain | 72-533 | Extracellular | ||||
Sequence: RTYLSWEVSVSLSLGFKTMDFPAVTICNASPFQYSKVKHLLRDLDELMEAVLERILAPEHSDANATRTLNVTMWNYTPLVLIDEQNPHCPVVLDLFGDIHNGSASSSPAPARSCTVHGCKVAMRLCSLNGTVCTFRNFTSATQAVTEWYLLQATNIFSQVPQRELVEMSYPAERLILACLFGAEPCSYRNFTSIFHPDYGNCYIFNWGMTEKALPSANPGAEFGLKLILDIGQEDYVPFLTSTAGARLMLHEQRSYPFIKDEGIYAMSGTETSIGVLVDRLERKGEPYSQCTVNGSDVPVRNLYSDYNTTYSIQACIRSCFQDHMIQNCSCAHYLYPLPRGERYCNNREFPDWAYCYSHLRMSVAQRETCINMCKESCNDTQYKMTISMADWPSEASEDWIFHVLSQERDQSTNITLSRKGVVKLNIYFQEFNYRTIEESAANNIVWLLSNLGGQFGFWMGG | ||||||
Transmembrane | 534-554 | Helical; Name=2 | ||||
Sequence: SVLCLIEFGEILIDFVWITII | ||||||
Topological domain | 555-641 | Cytoplasmic | ||||
Sequence: KLVAFAKSLRQKRAQARYAGPPPTVAELVEAHTNFGFQPDVARPGPDPGTYPDEQTLPIPGTPPPNYDSLRLQPLDVIESDSEGDAI |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000181267 | 1-641 | Amiloride-sensitive sodium channel subunit beta | |||
Sequence: MHLKKYLLKGLHRLQKGPGYSYKELLVWYCNNTNTHGPKRIICEGPKKKAMWFLITLLFTSLVCWQWGVFIRTYLSWEVSVSLSLGFKTMDFPAVTICNASPFQYSKVKHLLRDLDELMEAVLERILAPEHSDANATRTLNVTMWNYTPLVLIDEQNPHCPVVLDLFGDIHNGSASSSPAPARSCTVHGCKVAMRLCSLNGTVCTFRNFTSATQAVTEWYLLQATNIFSQVPQRELVEMSYPAERLILACLFGAEPCSYRNFTSIFHPDYGNCYIFNWGMTEKALPSANPGAEFGLKLILDIGQEDYVPFLTSTAGARLMLHEQRSYPFIKDEGIYAMSGTETSIGVLVDRLERKGEPYSQCTVNGSDVPVRNLYSDYNTTYSIQACIRSCFQDHMIQNCSCAHYLYPLPRGERYCNNREFPDWAYCYSHLRMSVAQRETCINMCKESCNDTQYKMTISMADWPSEASEDWIFHVLSQERDQSTNITLSRKGVVKLNIYFQEFNYRTIEESAANNIVWLLSNLGGQFGFWMGGSVLCLIEFGEILIDFVWITIIKLVAFAKSLRQKRAQARYAGPPPTVAELVEAHTNFGFQPDVARPGPDPGTYPDEQTLPIPGTPPPNYDSLRLQPLDVIESDSEGDAI | ||||||
Glycosylation | 141 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 261 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Modified residue | 634 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 636 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation.
N-glycosylated. N-glycosylation is required for interaction with BPIFA1.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in some organisms and can replace the alpha/SCNN1A subunit to form an alternative channel with specific properties. Interacts with NEDD4 (via WW domains). Interacts with NEDD4L (via WW domains). Interacts with WWP1 (via WW domains). Interacts with WWP2 (via WW domains). Interacts with the full-length immature form of PCSK9 (pro-PCSK9). Interacts (N-glycosylated) with BPIFA1; the interaction is direct and inhibits the proteolytic processing of SCNN1A and SCNN1G and the activation of ENaC.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 593-624 | Disordered | ||||
Sequence: PDVARPGPDPGTYPDEQTLPIPGTPPPNYDSL | ||||||
Compositional bias | 605-621 | Pro residues | ||||
Sequence: YPDEQTLPIPGTPPPNY |
Sequence similarities
Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1B subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length641
- Mass (Da)72,654
- Last updated2015-04-29 v2
- Checksum5A040E85895D751E
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 567 | in Ref. 2; AAB09034 | ||||
Sequence: R → Q | ||||||
Compositional bias | 605-621 | Pro residues | ||||
Sequence: YPDEQTLPIPGTPPPNY |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAEX03004450 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
U68062 EMBL· GenBank· DDBJ | AAB09034.1 EMBL· GenBank· DDBJ | Genomic DNA |