Q95107 · WASL_BOVIN

  • Protein
    Actin nucleation-promoting factor WASL
  • Gene
    WASL
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex (PubMed:17609109).
Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization. Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization (By similarity).
Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Plays a role in dendrite spine morphogenesis (By similarity).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoskeleton
Cellular Componentendoplasmic reticulum membrane
Cellular Componentnucleus
Molecular Functionactin binding
Molecular Functionidentical protein binding
Molecular Functionmicrotubule binding
Biological Processactin filament polymerization
Biological Processcell division
Biological Processdendritic spine morphogenesis
Biological Processpositive regulation of transcription by RNA polymerase II

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Actin nucleation-promoting factor WASL
  • Alternative names
    • Neural Wiskott-Aldrich syndrome protein (N-WASP)

Gene names

    • Name
      WASL

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos

Accessions

  • Primary accession
    Q95107

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Cytoplasm
Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated. Exported from the nucleus by an nuclear export signal (NES)-dependent mechanism to the cytoplasm.

Keywords

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylserine
ChainPRO_00001889992-505Actin nucleation-promoting factor WASL
Modified residue242Phosphoserine; by TNK2
Modified residue256Phosphotyrosine; by FAK1 and TNK2
Modified residue307Omega-N-methylarginine
Modified residue484Phosphoserine
Modified residue485Phosphoserine

Post-translational modification

Phosphorylation at Ser-242, Tyr-256, Ser-484 and Ser-485 enhances actin polymerization activity.

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Binds actin and the Arp2/3 complex. Interacts with CDC42 (By similarity).
Interacts with FCHSD1. Interacts with FCHSD2 (By similarity).
Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP (By similarity).
Interacts with SNX9 (PubMed:17609109).
Interacts with the WW domains of PRPF40A/FBP11. Interacts with PTK2/FAK1. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity).
Interacts with NOSTRIN. Binds to TNK2. Interacts with SNX33. Interacts with NONO (via second RRM domain); the interaction is direct. Component of a multiprotein complex with NONO and SFPQ; associates with the complex via direct interaction with NONO (By similarity).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q95107ACTR2 A7MB622EBI-6162776, EBI-6162748
XENO Q95107Cttn Q605982EBI-6162776, EBI-397955
BINARY Q95107WASL Q951073EBI-6162776, EBI-6162776

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain34-141WH1
Region138-163Disordered
Region185-205Disordered
Domain203-216CRIB
Region266-406Disordered
Compositional bias274-395Pro residues
Domain405-422WH2 1
Domain433-450WH2 2
Region449-468Disordered
Region477-505Disordered
Compositional bias486-505Acidic residues

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    505
  • Mass (Da)
    54,672
  • Last updated
    1997-02-01 v1
  • Checksum
    54B83B48F1CDB3B8
MSSGQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQINNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHSSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPGVGAPPPPPNRMYPPPLPALPSSAPSGPPPPPPPLSVSGSVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTPAGSKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVTDAPESTPPAPAPTSGIVGALMEVMQKRSKAIHSSDEDEDEDDDEDFEDDDEWED

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A3Q1M352A0A3Q1M352_BOVINWASL511

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias274-395Pro residues
Compositional bias486-505Acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D67066
EMBL· GenBank· DDBJ
BAA11082.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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