Q94K85 · CATB3_ARATH

Function

function

Thiol protease that possesses high activity toward the cathepsin synthetic substrate Arg-Arg-7-amino-4-methylcoumarin (RR-AMC) and the papain substrate Gly-Arg-Arg-AMC (GRR-AMC). Can cleave the papain substrate Phe-Arg-AMC (FR-AMC) and the caspase-3 substrate Asp-Glu-Val-Asp-rhodamine 110 (DEVD-R110). Has no activity towards the caspase-6 substrate VEID-AMC, caspase-8 substrate IETD-AMC and caspase-1 substrate YVAD-AMC (PubMed:27058316).
Plays a central role in plant programmed cell death (PCD). In addition to its role in protein degradation, may cleave and/or degrade a number of target proteins, activating signaling towards PCD. Contributes to the increase of caspase-3-like activity after UV-C-induced PCD and is required for abiotic stress-induced PCD (PubMed:27058316).
Functions redundantly with CATHB1 and CATHB2 in basal defense and distinct forms of plant programmed cell death (PCD). Participates in the establishment of basal resistance against the bacterial pathogen Pseudomonase syringae pv. tomato DC3000. Required for full levels of PCD during resistance (R) gene-mediated hypersensitive response (HR). Involved in the regulation of senescence, a developmental form of PCD in plants (PubMed:19453434).
May be involved in the degradation of seed storage proteins during seed germination (PubMed:24600022).

Activity regulation

Inhibited by the cathepsin B inhibitors Ac-LVK-CHO, CA-074 and Z-FA-FMK, and the caspase-3 inhibitor Z-DEVD-CHO.

pH Dependence

Optimum pH is 5.5.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site131
Active site286
Active site307

GO annotations

AspectTerm
Cellular Componentplant-type vacuole
Cellular Componentsecretory vesicle
Molecular Functioncysteine-type endopeptidase activity
Biological Processdefense response
Biological Processproteolysis

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Cathepsin B-like protease 3
  • EC number
  • Alternative names
    • Cathepsin B3
      (AtCathB3
      )

Gene names

    • Name
      CATHB3
    • ORF names
      T15B16.17a
    • Ordered locus names
      At4g01610

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q94K85
  • Secondary accessions
    • Q9ZSI0

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Delayed germination and decreased germination rate.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis131Loss of protease activity.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 24 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for signal, propeptide, glycosylation, chain, disulfide bond.

TypeIDPosition(s)Description
Signal1-26
PropeptidePRO_000043942127-102Activation peptide
Glycosylation69N-linked (GlcNAc...) asparagine
ChainPRO_0000439457102-342Cathepsin B-like protease 3
Disulfide bond116↔145
Disulfide bond128↔171
Glycosylation151N-linked (GlcNAc...) asparagine
Disulfide bond162↔216
Disulfide bond163↔167
Disulfide bond193↔220
Disulfide bond202↔207
PropeptidePRO_0000439422343-359Removed in mature form

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in root tips, root vasculature, emerging lateral root, hydathodes, vascular tissue of leaves, vasculature of sepals and anthers, stigma, and vascular bundles at the base and the upper part of the siliques.

Induction

By dark-induced senescence. Induced by infection with an avirulent strain of the bacterial pathogen Pseudomonase syringae pv. tomato DC3000.

Developmental stage

Expressed in developing siliques 13 to 15 days after pollination (DAP). In germinating seeds, expressed from 12 to 48 hours after imbibition with a peak of expression at 24 hours.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the peptidase C1 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 2 isoforms produced by Alternative splicing.

Q94K85-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    359
  • Mass (Da)
    39,418
  • Last updated
    2001-12-01 v1
  • Checksum
    D3199ABA3FEA831E
MAVYNTKLCLASVFLLLGLLLAFDLKGIEAESLTKQKLDSKILQDEIVKKVNENPNAGWKAAINDRFSNATVAEFKRLLGVKPTPKKHFLGVPIVSHDPSLKLPKAFDARTAWPQCTSIGNILDQGHCGSCWAFGAVESLSDRFCIQFGMNISLSVNDLLACCGFRCGDGCDGGYPIAAWQYFSYSGVVTEECDPYFDNTGCSHPGCEPAYPTPKCSRKCVSDNKLWSESKHYSVSTYTVKSNPQDIMAEVYKNGPVEVSFTVYEDFAHYKSGVYKHITGSNIGGHAVKLIGWGTSSEGEDYWLMANQWNRGWGDDGYFMIRRGTNECGIEDEPVAGLPSSKNVFRVDTGSNDLPVASV

Q94K85-2

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_058851124-125in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF083797
EMBL· GenBank· DDBJ
AAN60355.1
EMBL· GenBank· DDBJ
mRNA
AF104919
EMBL· GenBank· DDBJ
AAC72872.1
EMBL· GenBank· DDBJ
Genomic DNA
AL161492
EMBL· GenBank· DDBJ
CAB77732.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002687
EMBL· GenBank· DDBJ
AEE82052.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002687
EMBL· GenBank· DDBJ
AEE82053.1
EMBL· GenBank· DDBJ
Genomic DNA
AF370193
EMBL· GenBank· DDBJ
AAK44008.1
EMBL· GenBank· DDBJ
mRNA
AY065167
EMBL· GenBank· DDBJ
AAL38343.1
EMBL· GenBank· DDBJ
mRNA
AY114015
EMBL· GenBank· DDBJ
AAM45063.1
EMBL· GenBank· DDBJ
mRNA
BT001190
EMBL· GenBank· DDBJ
AAN65077.1
EMBL· GenBank· DDBJ
mRNA
AK175280
EMBL· GenBank· DDBJ
BAD43043.1
EMBL· GenBank· DDBJ
mRNA
AK175481
EMBL· GenBank· DDBJ
BAD43244.1
EMBL· GenBank· DDBJ
mRNA
AK175539
EMBL· GenBank· DDBJ
BAD43302.1
EMBL· GenBank· DDBJ
mRNA
AK176165
EMBL· GenBank· DDBJ
BAD43928.1
EMBL· GenBank· DDBJ
mRNA
AK176244
EMBL· GenBank· DDBJ
BAD44007.1
EMBL· GenBank· DDBJ
mRNA
AK176281
EMBL· GenBank· DDBJ
BAD44044.1
EMBL· GenBank· DDBJ
mRNA
AK176330
EMBL· GenBank· DDBJ
BAD44093.1
EMBL· GenBank· DDBJ
mRNA
AK176416
EMBL· GenBank· DDBJ
BAD44179.1
EMBL· GenBank· DDBJ
mRNA
AK176433
EMBL· GenBank· DDBJ
BAD44196.1
EMBL· GenBank· DDBJ
mRNA
AK176487
EMBL· GenBank· DDBJ
BAD44250.1
EMBL· GenBank· DDBJ
mRNA
AK221398
EMBL· GenBank· DDBJ
BAD94342.1
EMBL· GenBank· DDBJ
mRNA
AK230235
EMBL· GenBank· DDBJ
BAF02040.1
EMBL· GenBank· DDBJ
mRNA
AY086034
EMBL· GenBank· DDBJ
AAM63244.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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