Q94JV5 · NILP2_ARATH

Function

function

Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione (PubMed:30692244).
Possesses amidase activity toward deaminated ophthalmate in vitro (PubMed:30692244).

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
35 μMN-(4-oxoglutarate)-L-cysteinylglycine
6.7 μMophthalmate
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
7.9 μmol/min/mgwith N-(4-oxoglutarate)-L-cysteinylglycine as substrate
5.6 μmol/min/mgwith ophthalmate as substrate
kcat is 4.2 sec-1 with N-(4-oxoglutarate)-L-cysteinylglycine as substrate (PubMed:30692244).
kcat is 3.0 sec-1 with ophthalmate as substrate (PubMed:30692244).

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site76Proton acceptor
Active site147Proton donor
Active site188Nucleophile

GO annotations

AspectTerm
Cellular Componentchloroplast
Cellular Componentcytoplasm
Cellular Componentplastid
Molecular Function[acetyl-CoA carboxylase]-phosphatase activity
Molecular Functiondeaminated glutathione amidase activity
Biological Processmetabolite repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Deaminated glutathione amidase, chloroplastic/cytosolic
  • EC number
  • Short names
    dGSH amidase
  • Alternative names
    • Nitrilase-like protein 2
    • Protein nitrilase 1 homolog
      (AtNit1
      ; Protein Nit1 homolog
      )

Gene names

    • Name
      NLP2
    • Synonyms
      NIT1
    • ORF names
      T32A17.100
    • Ordered locus names
      At4g08790

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q94JV5
  • Secondary accessions
    • Q9LE50

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

No visible phenotype under normal growth conditions, but the accumuluation of N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione) in mutant plants is up to 70-fold higher than in the wild type.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis29No cytoplasmic localization.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 34 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for transit peptide, chain.

Type
IDPosition(s)Description
Transit peptide1-36Chloroplast
ChainPRO_000042670537-307Deaminated glutathione amidase, chloroplastic/cytosolic

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain37-286CN hydrolase

Sequence similarities

Belongs to the nitrilase superfamily. NIT1/NIT2 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 2 isoforms produced by Alternative initiation.

Q94JV5-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    1
  • Synonyms
    Full-length, FL
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    307
  • Mass (Da)
    33,971
  • Last updated
    2001-12-01 v1
  • MD5 Checksum
    5C1A8D3161CC346FE72EFC20D5177A66
MNAYSVSLDFTKPSLFTRITLSSQIPLTMATTVNKTVRVAAAQMTSVNDLMTNFATCSRLVQEAALAGAKLICFPENFSFVGDKEGESVKIAEPLDGPVMERYCSLARDSNIWLSLGGFQERFDDTHLCNTHVVIDDAGMIRDTYQKMHLFDVDVPGGSSYKESSFTVPGTKIVSVDSPVGRLGLTVCYDLRFPKIYQQLRFEQKAQVLLVPSAFTKVTGEAHWEILLRARAIETQCYVIAAAQAGKHNEKRESYGDTLIIDPWGTVVGRLPDRVSTGIVVADIDFSLIDSVRTKMPIDKQRVSIDL

Q94JV5-2

  • Name
    2
  • Synonyms
    Truncated, Tr
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Sequence caution

The sequence CAB78004.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence CAB82115.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_0603891-28in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL161512
EMBL· GenBank· DDBJ
CAB78004.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AL161813
EMBL· GenBank· DDBJ
CAB82115.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002687
EMBL· GenBank· DDBJ
AEE82678.1
EMBL· GenBank· DDBJ
Genomic DNA
AF372904
EMBL· GenBank· DDBJ
AAK49620.1
EMBL· GenBank· DDBJ
mRNA
AY133544
EMBL· GenBank· DDBJ
AAM91374.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice.
Help