Q94JQ4 · RIDA_ARATH
- ProteinReactive Intermediate Deaminase A, chloroplastic
- GeneRIDA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids187 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Hydrolyzes the Ser-derived enamine/imine product of Thr dehydratase, protecting the plastidial branched-chain aminotransferase BCAT3 (AC Q9M401) from inactivation. Involved in Ile biosynthesis.
Catalytic activity
- 2-iminobutanoate + H2O = 2-oxobutanoate + NH4+
Pathway
Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine.
Features
Showing features for site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 79 | Stabilizes the substrate | ||||
Sequence: Y | ||||||
Binding site | 165 | substrate | ||||
Sequence: R | ||||||
Site | 180 | Important for catalytic activity at high pH | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | chloroplast envelope | |
Cellular Component | chloroplast stroma | |
Cellular Component | cytosol | |
Cellular Component | plant-type vacuole | |
Cellular Component | plastid | |
Cellular Component | thylakoid | |
Molecular Function | 2-iminobutanoate deaminase activity | |
Molecular Function | 2-iminopropanoate deaminase activity | |
Molecular Function | deaminase activity | |
Biological Process | branched-chain amino acid biosynthetic process | |
Biological Process | isoleucine biosynthetic process | |
Biological Process | response to toxic substance |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameReactive Intermediate Deaminase A, chloroplastic
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ94JQ4
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Reduced root growth and increased sensitivity of the root to added Ser.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 165 | Loss of activity. | ||||
Sequence: R → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 12 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-58 | Chloroplast | ||||
Sequence: MTWSVFRSINTPTLDLSTALRSTRTPLVAAGVGCATFAGVSLFRMSSRSPPFASLSVS | ||||||
Chain | PRO_0000430561 | 59-187 | Reactive Intermediate Deaminase A, chloroplastic | |||
Sequence: ASSVKKEVVSTEKAPAALGPYSQAIKANNLVFLSGVLGLIPETGKFVSESVEDQTEQVLKNMGEILKASGADYSSVVKTTIMLADLADFKTVNEIYAKYFPAPSPARSTYQVAALPLNAKIEIECIATL |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in leaves, petiols, petals, carpels and shoot apex.
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length187
- Mass (Da)19,816
- Last updated2001-12-01 v1
- Checksum40C19BA9C8EF2F44
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1I9LS34 | A0A1I9LS34_ARATH | RIDA | 255 |
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB024036 EMBL· GenBank· DDBJ | BAB02821.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002686 EMBL· GenBank· DDBJ | AEE76372.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF375446 EMBL· GenBank· DDBJ | AAK53030.1 EMBL· GenBank· DDBJ | mRNA | ||
AY060547 EMBL· GenBank· DDBJ | AAL31178.1 EMBL· GenBank· DDBJ | mRNA | ||
AY086036 EMBL· GenBank· DDBJ | AAM63246.1 EMBL· GenBank· DDBJ | mRNA | ||
AK227774 EMBL· GenBank· DDBJ | BAE99756.1 EMBL· GenBank· DDBJ | mRNA | ||
AK229768 EMBL· GenBank· DDBJ | BAF01604.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |