Q94JM3 · ARFB_ARATH
- ProteinAuxin response factor 2
- GeneARF2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids859 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Could act as transcriptional activator or repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Promotes flowering, stamen development, floral organ abscission and fruit dehiscence. Functions independently of ethylene and cytokinin response pathways. May act as a repressor of cell division and organ growth.
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 164-266 | TF-B3 | ||||
Sequence: FCKTLTASDTSTHGGFSVLRRHADECLPPLDMSRQPPTQELVAKDLHANEWRFRHIFRGQPRRHLLQSGWSVFVSSKRLVAGDAFIFLRGENGELRVGVRRAM |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | DNA-binding transcription factor activity | |
Molecular Function | sequence-specific DNA binding | |
Biological Process | auxin-activated signaling pathway | |
Biological Process | floral organ abscission | |
Biological Process | fruit dehiscence | |
Biological Process | leaf senescence | |
Biological Process | negative regulation of cell population proliferation | |
Biological Process | negative regulation of DNA-templated transcription | |
Biological Process | plant ovule development | |
Biological Process | positive regulation of flower development | |
Biological Process | positive regulation of potassium ion import across plasma membrane | |
Biological Process | response to abscisic acid |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameAuxin response factor 2
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ94JM3
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Large, dark green rosette leaves, delayed flowering, thick and long inflorescence, abnormal flower morphology and sterility in early formed flowers, but fertility in late-formed flowers. Delayed senescence and abscission. Increased seed size and weight, and extra cell division and expansion in many organs.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 51 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000111506 | 1-859 | Auxin response factor 2 | |||
Sequence: MASSEVSMKGNRGGDNFSSSGFSDPKETRNVSVAGEGQKSNSTRSAAAERALDPEAALYRELWHACAGPLVTVPRQDDRVFYFPQGHIEQVEASTNQAAEQQMPLYDLPSKLLCRVINVDLKAEADTDEVYAQITLLPEANQDENAIEKEAPLPPPPRFQVHSFCKTLTASDTSTHGGFSVLRRHADECLPPLDMSRQPPTQELVAKDLHANEWRFRHIFRGQPRRHLLQSGWSVFVSSKRLVAGDAFIFLRGENGELRVGVRRAMRQQGNVPSSVISSHSMHLGVLATAWHAISTGTMFTVYYKPRTSPSEFIVPFDQYMESVKNNYSIGMRFKMRFEGEEAPEQRFTGTIVGIEESDPTRWPKSKWRSLKVRWDETSSIPRPDRVSPWKVEPALAPPALSPVPMPRPKRPRSNIAPSSPDSSMLTREGTTKANMDPLPASGLSRVLQGQEYSTLRTKHTESVECDAPENSVVWQSSADDDKVDVVSGSRRYGSENWMSSARHEPTYTDLLSGFGTNIDPSHGQRIPFYDHSSSPSMPAKRILSDSEGKFDYLANQWQMIHSGLSLKLHESPKVPAATDASLQGRCNVKYSEYPVLNGLSTENAGGNWPIRPRALNYYEEVVNAQAQAQAREQVTKQPFTIQEETAKSREGNCRLFGIPLTNNMNGTDSTMSQRNNLNDAAGLTQIASPKVQDLSDQSKGSKSTNDHREQGRPFQTNNPHPKDAQTKTNSSRSCTKVHKQGIALGRSVDLSKFQNYEELVAELDRLFEFNGELMAPKKDWLIVYTDEENDMMLVGDDPWQEFCCMVRKIFIYTKEEVRKMNPGTLSCRSEEEAVVGEGSDAKDAKSASNPSLSSAGNS |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the whole plant.
Developmental stage
Expressed in the sepals and carpels of young flower buds. At stage 10 of flower development, expression in the carpels becomes restricted to the style. Also expressed in anthers and filaments. At stage 13, expressed in the region at the top of the pedicel, including the abscission zone. Expressed in developing siliques.
Gene expression databases
Interaction
Subunit
Homodimers and heterodimers. Interacts with ARF1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q94JM3 | ARF1 Q8L7G0 | 4 | EBI-1799262, EBI-2324259 | |
BINARY | Q94JM3 | ASK7 Q39011 | 6 | EBI-1799262, EBI-1798250 | |
BINARY | Q94JM3 | IAA10 Q38828 | 6 | EBI-1799262, EBI-3946434 | |
BINARY | Q94JM3 | IAA17 P93830 | 3 | EBI-1799262, EBI-632243 | |
BINARY | Q94JM3 | IAA18 O24408 | 3 | EBI-1799262, EBI-2295525 | |
BINARY | Q94JM3 | IAA26 Q8LAL2 | 7 | EBI-1799262, EBI-3947418 | |
BINARY | Q94JM3 | IAA29 Q93WC4 | 6 | EBI-1799262, EBI-3946697 | |
BINARY | Q94JM3 | IAA32 Q8RYC6 | 6 | EBI-1799262, EBI-3946448 | |
BINARY | Q94JM3 | IAA33 Q9FKM7 | 3 | EBI-1799262, EBI-3946739 | |
BINARY | Q94JM3 | IAA34 Q9C5X0 | 6 | EBI-1799262, EBI-3946459 | |
BINARY | Q94JM3 | TCP23 Q9LQF0 | 3 | EBI-1799262, EBI-15192297 | |
BINARY | Q94JM3 | WRKY38 Q8GWF1 | 3 | EBI-1799262, EBI-1993263 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-47 | Polar residues | ||||
Sequence: MASSEVSMKGNRGGDNFSSSGFSDPKETRNVSVAGEGQKSNSTRSAA | ||||||
Region | 1-48 | Disordered | ||||
Sequence: MASSEVSMKGNRGGDNFSSSGFSDPKETRNVSVAGEGQKSNSTRSAAA | ||||||
Compositional bias | 396-414 | Pro residues | ||||
Sequence: LAPPALSPVPMPRPKRPRS | ||||||
Region | 396-442 | Disordered | ||||
Sequence: LAPPALSPVPMPRPKRPRSNIAPSSPDSSMLTREGTTKANMDPLPAS | ||||||
Compositional bias | 416-431 | Polar residues | ||||
Sequence: IAPSSPDSSMLTREGT | ||||||
Region | 687-736 | Disordered | ||||
Sequence: IASPKVQDLSDQSKGSKSTNDHREQGRPFQTNNPHPKDAQTKTNSSRSCT | ||||||
Compositional bias | 711-736 | Polar residues | ||||
Sequence: QGRPFQTNNPHPKDAQTKTNSSRSCT | ||||||
Domain | 733-817 | PB1 | ||||
Sequence: RSCTKVHKQGIALGRSVDLSKFQNYEELVAELDRLFEFNGELMAPKKDWLIVYTDEENDMMLVGDDPWQEFCCMVRKIFIYTKEE | ||||||
Region | 829-859 | Disordered | ||||
Sequence: RSEEEAVVGEGSDAKDAKSASNPSLSSAGNS | ||||||
Compositional bias | 845-859 | Polar residues | ||||
Sequence: AKSASNPSLSSAGNS |
Domain
Interactions between auxin response factors (ARFs) and Aux/IAA proteins occur through their C-terminal dimerization domains III and IV.
Sequence similarities
Belongs to the ARF family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
Q94JM3-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length859
- Mass (Da)95,701
- Last updated2004-04-13 v2
- Checksum6B75171D7FF0E66B
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F4K536 | F4K536_ARATH | ARF2 | 853 |
Sequence caution
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-47 | Polar residues | ||||
Sequence: MASSEVSMKGNRGGDNFSSSGFSDPKETRNVSVAGEGQKSNSTRSAA | ||||||
Compositional bias | 396-414 | Pro residues | ||||
Sequence: LAPPALSPVPMPRPKRPRS | ||||||
Compositional bias | 416-431 | Polar residues | ||||
Sequence: IAPSSPDSSMLTREGT | ||||||
Sequence conflict | 491-492 | in Ref. 6; AAC49752 and 7; CAD29696/CAD30210 | ||||
Sequence: RR → KK | ||||||
Sequence conflict | 520 | in Ref. 6; AAC49752 and 7; CAD29696/CAD30210 | ||||
Sequence: D → E | ||||||
Sequence conflict | 596 | in Ref. 5; BAD93968 | ||||
Sequence: V → A | ||||||
Sequence conflict | 675 | in Ref. 6; AAC49752 and 7; CAD29696/CAD30210 | ||||
Sequence: R → K | ||||||
Compositional bias | 711-736 | Polar residues | ||||
Sequence: QGRPFQTNNPHPKDAQTKTNSSRSCT | ||||||
Sequence conflict | 823 | in Ref. 6; AAC49752 and 7; CAD29696/CAD30210 | ||||
Sequence: P → Q | ||||||
Sequence conflict | 826 | in Ref. 5; BAD93959/BAD93897/BAD93891 | ||||
Sequence: L → V | ||||||
Compositional bias | 845-859 | Polar residues | ||||
Sequence: AKSASNPSLSSAGNS |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY669787 EMBL· GenBank· DDBJ | AAT67071.1 EMBL· GenBank· DDBJ | mRNA | ||
AB016880 EMBL· GenBank· DDBJ | BAB10162.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED97548.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED97549.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED97550.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | ANM68885.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF336918 EMBL· GenBank· DDBJ | AAG53999.1 EMBL· GenBank· DDBJ | mRNA | ||
AF378862 EMBL· GenBank· DDBJ | AAK55665.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
BT000784 EMBL· GenBank· DDBJ | AAN31923.1 EMBL· GenBank· DDBJ | mRNA | ||
BT001072 EMBL· GenBank· DDBJ | AAN46837.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AK221252 EMBL· GenBank· DDBJ | BAD93891.1 EMBL· GenBank· DDBJ | mRNA | ||
AK221254 EMBL· GenBank· DDBJ | BAD93897.1 EMBL· GenBank· DDBJ | mRNA | ||
AK221274 EMBL· GenBank· DDBJ | BAD93959.1 EMBL· GenBank· DDBJ | mRNA | ||
AK221277 EMBL· GenBank· DDBJ | BAD93968.1 EMBL· GenBank· DDBJ | mRNA | ||
AK221282 EMBL· GenBank· DDBJ | BAD93985.1 EMBL· GenBank· DDBJ | mRNA | ||
AK221305 EMBL· GenBank· DDBJ | BAD94058.1 EMBL· GenBank· DDBJ | mRNA | ||
U89771 EMBL· GenBank· DDBJ | AAC49752.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AJ441313 EMBL· GenBank· DDBJ | CAD29696.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AJ458328 EMBL· GenBank· DDBJ | CAD30210.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |