Q94IW5 · C90D2_ORYSJ
- ProteinCytochrome P450 90D2
- GeneCYP90D2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids490 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the C6-oxidation step in brassinosteroids biosynthesis (PubMed:14615594).
May convert 6-deoxoteasterone (6-deoxoTE) to 3-dehydro-6-deoxoteasterone (6-deoxo3DT, 6-deoxo3DHT), and teasterone (TE) to 3-dehydroteasterone (3DT, 3-DHT) (PubMed:14615594).
Involved in the elongation of leaf sheaths and stems (PubMed:14615594).
May convert 6-deoxoteasterone (6-deoxoTE) to 3-dehydro-6-deoxoteasterone (6-deoxo3DT, 6-deoxo3DHT), and teasterone (TE) to 3-dehydroteasterone (3DT, 3-DHT) (PubMed:14615594).
Involved in the elongation of leaf sheaths and stems (PubMed:14615594).
Catalytic activity
- 6-deoxoteasterone + O2 + reduced [NADPH--hemoprotein reductase] = 3-dehydro-6-deoxoteasterone + H+ + 2 H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
Cofactor
Pathway
Plant hormone biosynthesis; brassinosteroid biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen | |
Biological Process | brassinosteroid biosynthetic process | |
Biological Process | brassinosteroid homeostasis | |
Biological Process | sterol metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytochrome P450 90D2
- Short namesOsCYP90D2
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ94IW5
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 4-24 | Helical | ||||
Sequence: AAAGWAAPAFAVAAVVIWVVL |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Plants show abnormalities in growth: inhibition of second internode elongation, shortened leaf sheaths, erect leaves, and deficiency in skotomorphogenesis. Treatment with exogenous brassinolide (BL) rescues the abnormal phenotype.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 305 | In d2-2; dwarf and defective in brassinosteroid biosynthesis. | ||||
Sequence: P → S |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000052187 | 1-490 | Cytochrome P450 90D2 | |||
Sequence: MVSAAAGWAAPAFAVAAVVIWVVLCSELLRRRRRGAGSGKGDAAAAARLPPGSFGWPVVGETLEFVSCAYSPRPEAFVDKRRKLHGSAVFRSHLFGSATVVTADAEVSRFVLQSDARAFVPWYPRSLTELMGKSSILLINGALQRRVHGLVGAFFKSSHLKSQLTADMRRRLSPALSSFPDSSLLHVQHLAKSVVFEILVRGLIGLEAGEEMQQLKQQFQEFIVGLMSLPIKLPGTRLYRSLQAKKKMARLIQRIIREKRARRAAASPPRDAIDVLIGDGSDELTDELISDNMIDLMIPAEDSVPVLITLAVKFLSECPLALHQLEEENIQLKRRKTDMGETLQWTDYMSLSFTQHVITETLRLGNIIGGIMRKAVRDVEVKGHLIPKGWCVFVYFRSVHLDDTLYDEPYKFNPWRWKEKDMSNGSFTPFGGGQRLCPGLDLARLEASIFLHHLVTSFRWVAEEDHIVNFPTVRLKRGMPIRVTAKEDDD |
Proteomic databases
Expression
Tissue specificity
Expressed at low levels leaf blades, shoot apex and elongating stem.
Induction
Down-regulated by brassinolide (BL) in ebisu dwarf (d2) mutant.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length490
- Mass (Da)54,825
- Last updated2001-12-01 v1
- Checksum9EC2853BBAFBB88F
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0P0UZ85 | A0A0P0UZ85_ORYSJ | Os01g0197100 | 243 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP003244 EMBL· GenBank· DDBJ | BAB56089.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP008207 EMBL· GenBank· DDBJ | BAF04211.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP014957 EMBL· GenBank· DDBJ | BAS70868.1 EMBL· GenBank· DDBJ | Genomic DNA |