Q94IA6 · C90D1_ARATH
- Protein3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1
- GeneCYP90D1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids491 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in brassinosteroid (BR) biosynthesis (PubMed:15703058, PubMed:17138693).
May convert teasterone (TE) to 3-dehydroteasterone (3DT, 3-DHT), or 6-deoxoteasterone (6-deoxoTE) to 3-dehydro-6-deoxoteasterone (6-deoxo3DT, 6-deoxo3DHT) (PubMed:15703058).
C-23 hydroxylase that converts directly (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one and 3-epi-6-deoxocathasterone to 3-dehydro-6-deoxoteasterone (6-deoxo3DT, 6-deoxo3DHT) and 6-deoxotyphasterol (6-deoxoTY), respectively (PubMed:17138693).
These C-23 hydroxylation shortcuts bypass campestanol, 6-deoxocathasterone, and 6-deoxoteasterone (6-deoxoTE) (PubMed:17138693).
Catalyzes also the conversion of cathasterone to teasterone (TE), 6-deoxotyphasterol (6-deoxoTY) to 6-deoxocathasterone (6-deoxoCT), (22S,24R)-22-hydroxyergost-4-en-3-one (22-OH-4-en-3-one) to (22R,23R)-22,23-dihydroxy-campest-4-en-3-one (22,23-diOH-4-en-3-one) and (22S)-22-hydroxycampesterol (22-OHCR) to (22R,23R)-22,23-dihydroxycampesterol (22,23-diOHCR) (PubMed:17138693).
May convert teasterone (TE) to 3-dehydroteasterone (3DT, 3-DHT), or 6-deoxoteasterone (6-deoxoTE) to 3-dehydro-6-deoxoteasterone (6-deoxo3DT, 6-deoxo3DHT) (PubMed:15703058).
C-23 hydroxylase that converts directly (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one and 3-epi-6-deoxocathasterone to 3-dehydro-6-deoxoteasterone (6-deoxo3DT, 6-deoxo3DHT) and 6-deoxotyphasterol (6-deoxoTY), respectively (PubMed:17138693).
These C-23 hydroxylation shortcuts bypass campestanol, 6-deoxocathasterone, and 6-deoxoteasterone (6-deoxoTE) (PubMed:17138693).
Catalyzes also the conversion of cathasterone to teasterone (TE), 6-deoxotyphasterol (6-deoxoTY) to 6-deoxocathasterone (6-deoxoCT), (22S,24R)-22-hydroxyergost-4-en-3-one (22-OH-4-en-3-one) to (22R,23R)-22,23-dihydroxy-campest-4-en-3-one (22,23-diOH-4-en-3-one) and (22S)-22-hydroxycampesterol (22-OHCR) to (22R,23R)-22,23-dihydroxycampesterol (22,23-diOHCR) (PubMed:17138693).
Catalytic activity
- 3-epi-6-deoxocathasterone + O2 + reduced [NADPH--hemoprotein reductase] = 6-deoxotyphasterol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
18.1 μM | (22S)-22-hydroxycampesterol | |||||
0.73 μM | (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one | |||||
0.77 μM | (22S,24R)-22-hydroxyergost-4-en-3-one | |||||
1.06 μM | 3-epi-6-deoxocathasterone | |||||
16.9 μM | 6-deoxocathasterone |
kcat is 0.12 min-1 for (22S)-22-hydroxycampesterol (PubMed:17138693).
kcat is 1.27 min-1 for (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one (PubMed:17138693).
kcat is 1.01 min-1 for (22S,24R)-22-hydroxyergost-4-en-3-one (PubMed:17138693).
kcat is 1.10 min-1 for 3-epi-6-deoxocathasterone (PubMed:17138693).
kcat is 0.29 min-1 for 6-deoxocathasterone (PubMed:17138693).
kcat is 1.27 min-1 for (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one (PubMed:17138693).
kcat is 1.01 min-1 for (22S,24R)-22-hydroxyergost-4-en-3-one (PubMed:17138693).
kcat is 1.10 min-1 for 3-epi-6-deoxocathasterone (PubMed:17138693).
kcat is 0.29 min-1 for 6-deoxocathasterone (PubMed:17138693).
Pathway
Plant hormone biosynthesis; brassinosteroid biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | (22S)-22-hydroxy-5alpha-campestan-3-one C-23 hydroxylase activity | |
Molecular Function | 3-epi-6-deoxocathasterone C-23 hydroxylase activity | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen | |
Biological Process | brassinosteroid biosynthetic process | |
Biological Process | leaf development | |
Biological Process | petal development | |
Biological Process | stamen development |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1
- EC number
- Short names3-dehydro-6-deoxoteasterone synthase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ94IA6
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 7-27 | Helical | ||||
Sequence: LLFFSFFFFIIIVIFNKINGL |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
No visible phenotype under normal growth conditions, but the double mutant plants cyp90c1 and cyp90d1 exhibit a characteristic brassinosteroid-deficient dwarf phenotype.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 37 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000394999 | 1-491 | 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 | |||
Sequence: MDTSSSLLFFSFFFFIIIVIFNKINGLRSSPASKKKLNDHHVTSQSHGPKFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVNRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDMHKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLVTRFRWIAEEDTIINFPTVHMKNKLPIWIKRI |
Proteomic databases
Expression
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length491
- Mass (Da)56,153
- Last updated2001-12-01 v1
- Checksum02FB908A91995A40
Computationally mapped potential isoform sequences
There are 7 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1I9LL75 | A0A1I9LL75_ARATH | RNC4 | 267 | ||
A0A1I9LL76 | A0A1I9LL76_ARATH | RNC4 | 175 | ||
A0A1I9LL77 | A0A1I9LL77_ARATH | RNC4 | 160 | ||
A0A1I9LL78 | A0A1I9LL78_ARATH | RNC4 | 197 | ||
A0A178VBI6 | A0A178VBI6_ARATH | CYP90D1 | 464 | ||
F4JEI2 | F4JEI2_ARATH | RNC4 | 172 | ||
Q94F12 | Q94F12_ARATH | RNC4 | 247 |
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB066286 EMBL· GenBank· DDBJ | BAB62109.1 EMBL· GenBank· DDBJ | mRNA | ||
AP001307 EMBL· GenBank· DDBJ | BAB01922.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002686 EMBL· GenBank· DDBJ | AEE75404.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT004084 EMBL· GenBank· DDBJ | AAO42111.1 EMBL· GenBank· DDBJ | mRNA | ||
BT005093 EMBL· GenBank· DDBJ | AAO50626.1 EMBL· GenBank· DDBJ | mRNA |