Q94IA6 · C90D1_ARATH

Function

function

Involved in brassinosteroid (BR) biosynthesis (PubMed:15703058, PubMed:17138693).
May convert teasterone (TE) to 3-dehydroteasterone (3DT, 3-DHT), or 6-deoxoteasterone (6-deoxoTE) to 3-dehydro-6-deoxoteasterone (6-deoxo3DT, 6-deoxo3DHT) (PubMed:15703058).
C-23 hydroxylase that converts directly (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one and 3-epi-6-deoxocathasterone to 3-dehydro-6-deoxoteasterone (6-deoxo3DT, 6-deoxo3DHT) and 6-deoxotyphasterol (6-deoxoTY), respectively (PubMed:17138693).
These C-23 hydroxylation shortcuts bypass campestanol, 6-deoxocathasterone, and 6-deoxoteasterone (6-deoxoTE) (PubMed:17138693).
Catalyzes also the conversion of cathasterone to teasterone (TE), 6-deoxotyphasterol (6-deoxoTY) to 6-deoxocathasterone (6-deoxoCT), (22S,24R)-22-hydroxyergost-4-en-3-one (22-OH-4-en-3-one) to (22R,23R)-22,23-dihydroxy-campest-4-en-3-one (22,23-diOH-4-en-3-one) and (22S)-22-hydroxycampesterol (22-OHCR) to (22R,23R)-22,23-dihydroxycampesterol (22,23-diOHCR) (PubMed:17138693).

Catalytic activity

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
18.1 μM(22S)-22-hydroxycampesterol
0.73 μM(22S,24R)-22-hydroxy-5-alpha-ergostan-3-one
0.77 μM(22S,24R)-22-hydroxyergost-4-en-3-one
1.06 μM3-epi-6-deoxocathasterone
16.9 μM6-deoxocathasterone
kcat is 0.12 min-1 for (22S)-22-hydroxycampesterol (PubMed:17138693).
kcat is 1.27 min-1 for (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one (PubMed:17138693).
kcat is 1.01 min-1 for (22S,24R)-22-hydroxyergost-4-en-3-one (PubMed:17138693).
kcat is 1.10 min-1 for 3-epi-6-deoxocathasterone (PubMed:17138693).
kcat is 0.29 min-1 for 6-deoxocathasterone (PubMed:17138693).

Pathway

Plant hormone biosynthesis; brassinosteroid biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site442Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Molecular Function(22S)-22-hydroxy-5alpha-campestan-3-one C-23 hydroxylase activity
Molecular Function3-epi-6-deoxocathasterone C-23 hydroxylase activity
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
Biological Processbrassinosteroid biosynthetic process
Biological Processleaf development
Biological Processpetal development
Biological Processstamen development

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1
  • EC number
  • Short names
    3-dehydro-6-deoxoteasterone synthase
  • Alternative names
    • (22R,23R)-22,23-dihydroxy-campest-4-en-3-one synthase
      (EC:1.14.14.-
      ) . EC:1.14.14.- (UniProtKB | ENZYME | Rhea)
    • (22R,23R)-22,23-dihydroxycampesterol synthase
      (EC:1.14.14.-
      ) . EC:1.14.14.- (UniProtKB | ENZYME | Rhea)
    • 6-deoxoteasterone synthase
      (EC:1.14.14.-
      ) . EC:1.14.14.- (UniProtKB | ENZYME | Rhea)
    • Cytochrome P450 90D1
    • Teasterone synthase
      (EC:1.14.14.-
      ) . EC:1.14.14.- (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      CYP90D1
    • ORF names
      MMM17.15
    • Ordered locus names
      At3g13730

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q94IA6
  • Secondary accessions
    • Q9LIC5

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane7-27Helical

Keywords

Phenotypes & Variants

Disruption phenotype

No visible phenotype under normal growth conditions, but the double mutant plants cyp90c1 and cyp90d1 exhibit a characteristic brassinosteroid-deficient dwarf phenotype.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 37 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003949991-4913-epi-6-deoxocathasterone 23-monooxygenase CYP90D1

Proteomic databases

Expression

Tissue specificity

Expressed in leaf vascular tissue.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    491
  • Mass (Da)
    56,153
  • Last updated
    2001-12-01 v1
  • Checksum
    02FB908A91995A40
MDTSSSLLFFSFFFFIIIVIFNKINGLRSSPASKKKLNDHHVTSQSHGPKFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVNRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDMHKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLVTRFRWIAEEDTIINFPTVHMKNKLPIWIKRI

Computationally mapped potential isoform sequences

There are 7 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1I9LL75A0A1I9LL75_ARATHRNC4267
A0A1I9LL76A0A1I9LL76_ARATHRNC4175
A0A1I9LL77A0A1I9LL77_ARATHRNC4160
A0A1I9LL78A0A1I9LL78_ARATHRNC4197
A0A178VBI6A0A178VBI6_ARATHCYP90D1464
F4JEI2F4JEI2_ARATHRNC4172
Q94F12Q94F12_ARATHRNC4247

Sequence caution

The sequence BAB01922.1 differs from that shown. Reason: Erroneous gene model prediction

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB066286
EMBL· GenBank· DDBJ
BAB62109.1
EMBL· GenBank· DDBJ
mRNA
AP001307
EMBL· GenBank· DDBJ
BAB01922.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002686
EMBL· GenBank· DDBJ
AEE75404.1
EMBL· GenBank· DDBJ
Genomic DNA
BT004084
EMBL· GenBank· DDBJ
AAO42111.1
EMBL· GenBank· DDBJ
mRNA
BT005093
EMBL· GenBank· DDBJ
AAO50626.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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