Q94EG3 · NEC1_NICLS
- ProteinNectarin-1
- GeneNECI
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids229 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
May interact with bacterial adhesins thereby protecting the reproductive tissues from microbial attack. Has no oxalate oxidase activity.
Catalytic activity
- 2 superoxide + 2 H+ = H2O2 + O2
Cofactor
Note: Binds 1 Mn2+ ion per monomer.
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | apoplast | |
Cellular Component | plasmodesma | |
Molecular Function | manganese ion binding | |
Molecular Function | superoxide dismutase activity | |
Biological Process | plasmodesmata-mediated intercellular transport | |
Biological Process | regulation of root development |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNectarin-1
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Nicotianoideae > Nicotianeae > Nicotiana
Accessions
- Primary accessionQ94EG3
Subcellular Location
UniProt Annotation
GO Annotation
Note: Found in the nectar.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for signal, chain, disulfide bond, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-32 | |||||
Sequence: MAAFGINSKIFQSMEMAILFLLAISIDRYCFA | ||||||
Chain | PRO_0000010840 | 33-229 | Nectarin-1 | |||
Sequence: ADEDMLQDVCVADLHSKVKVNGFPCKTNFTAADFSSLAISKPGATNNKFGSVVTTANVEQVPGLNTLGVSLARIDYAPGGINPPHTHPRASEMVFVMEGELDVGFITTANVLVSKKIIKGEVFVFPRGLVHFQKNNGEVPAAVISAFNSQLPGTQSIPITLFGASPPVPDDVLAQTFQINTEDVQQIKSKFAPVKKF | ||||||
Disulfide bond | 42↔57 | |||||
Sequence: CVADLHSKVKVNGFPC | ||||||
Glycosylation | 60 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
PTM databases
Expression
Tissue specificity
Nectary tissues and to a lower level ovary. Not detected in petals, stems, leaves, roots or other floral tissues.
Developmental stage
Expressed during nectar secretion, while the flowers remain open.
Interaction
Subunit
Monomer. In the absence of manganese, it forms tetrameric and pentameric forms which show superoxide dismutase activity.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 69-217 | Cupin type-1 | ||||
Sequence: LAISKPGATNNKFGSVVTTANVEQVPGLNTLGVSLARIDYAPGGINPPHTHPRASEMVFVMEGELDVGFITTANVLVSKKIIKGEVFVFPRGLVHFQKNNGEVPAAVISAFNSQLPGTQSIPITLFGASPPVPDDVLAQTFQINTEDVQ |
Sequence similarities
Belongs to the germin family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length229
- Mass (Da)24,622
- Last updated2001-12-01 v1
- ChecksumAEC7C89FD3E1AF63
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 59 | in Ref. 2; AA sequence | ||||
Sequence: T → A |
Mass Spectrometry
Keywords
- Technical term