Q94CF0 · EHD1_ARATH
- ProteinEH domain-containing protein 1
- GeneEHD1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids545 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in endocytosis positive regulation. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes (PubMed:18547399, PubMed:23342166).
Confers salt tolerance (PubMed:23342166).
Confers salt tolerance (PubMed:23342166).
Catalytic activity
- GTP + H2O = GDP + H+ + phosphate
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 27 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 29 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 31 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 33 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 38 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 61 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 67 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 72 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 204-211 | GTP (UniProtKB | ChEBI) | ||||
Sequence: GQYSTGKT | ||||||
Binding site | 309-313 | GTP (UniProtKB | ChEBI) | ||||
Sequence: DFTGV | ||||||
Binding site | 359 | GTP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 395-398 | GTP (UniProtKB | ChEBI) | ||||
Sequence: SFSD |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | endosome | |
Cellular Component | endosome membrane | |
Cellular Component | intracellular membrane-bounded organelle | |
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Cellular Component | plasmodesma | |
Molecular Function | calcium ion binding | |
Molecular Function | G protein activity | |
Molecular Function | GTP binding | |
Biological Process | endocytic recycling | |
Biological Process | endocytosis | |
Biological Process | hyperosmotic salinity response | |
Biological Process | protein homooligomerization |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEH domain-containing protein 1
- EC number
- Short namesAtEHD1
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ94CF0
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Endosome membrane ; Peripheral membrane protein
Cell membrane ; Peripheral membrane protein
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Early flowering in short-day growth conditions (PubMed:18547399).
Slightly slower endocytosis with delayed internalization (PubMed:18547399, PubMed:23342166).
Reduced Brefeldin A sensitivity (PubMed:23342166).
Slightly slower endocytosis with delayed internalization (PubMed:18547399, PubMed:23342166).
Reduced Brefeldin A sensitivity (PubMed:23342166).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 1-93 | Impaired endosomal subcellular location and delayed internalization. | ||||
Sequence: Missing |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 27 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000431805 | 1-545 | EH domain-containing protein 1 | |||
Sequence: MEIESVAAGSCSKENQMIYKEWFEFSDSDGDGRITGNDAIKFFTMSNLPRPELKQIWAIADSKRQGYLGFKEFIVAMQLVSLAQTGHEISHEVLISDVDFKNINPPTMEGLGVLMAKKKHSSKSSDPNMNGSPAADTSLTAHWFSSKSSKKISLSSVTSIVDGLKRLYIQKLKPLEVAYRFNDFVSPLLTNSDFDAKPMVMLLGQYSTGKTTFIKHLLKSTYPGAHIGPEPTTDRFVVVMSGPDERSIPGNTVAVQADMPFSGLTTFGTAFLSKFECSQMPHPLLEHVTFVDTPGVLSGEKQRTQRAYDFTGVTSWFASKCDLILLLFDPHKLDVSDEFKRVISSLRGHDDKIRVVLNKADQVDTQQLMRVYGALMWSLGKVLNTPEVSRVYIGSFSDKPINEAATGPIGRELFEKEQDDLLADLKDIPKKACDRRINEFVKRARAAKIHAYIISHLKKEMPAIMGKAKAQQKLIDNLEDEFGKVQREHHLPKGDFPNVDHFREVLSGYNIDKFEKLKPKMLQTVDDMLGYDIPELLKNFKNPYD |
Proteomic databases
Expression
Structure
Family & Domains
Features
Showing features for domain, region, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 14-49 | EF-hand 1 | ||||
Sequence: ENQMIYKEWFEFSDSDGDGRITGNDAIKFFTMSNLP | ||||||
Domain | 15-93 | EH | ||||
Sequence: NQMIYKEWFEFSDSDGDGRITGNDAIKFFTMSNLPRPELKQIWAIADSKRQGYLGFKEFIVAMQLVSLAQTGHEISHEV | ||||||
Domain | 50-83 | EF-hand 2 | ||||
Sequence: RPELKQIWAIADSKRQGYLGFKEFIVAMQLVSLA | ||||||
Domain | 194-429 | Dynamin-type G | ||||
Sequence: FDAKPMVMLLGQYSTGKTTFIKHLLKSTYPGAHIGPEPTTDRFVVVMSGPDERSIPGNTVAVQADMPFSGLTTFGTAFLSKFECSQMPHPLLEHVTFVDTPGVLSGEKQRTQRAYDFTGVTSWFASKCDLILLLFDPHKLDVSDEFKRVISSLRGHDDKIRVVLNKADQVDTQQLMRVYGALMWSLGKVLNTPEVSRVYIGSFSDKPINEAATGPIGRELFEKEQDDLLADLKDIP | ||||||
Region | 204-211 | G1 motif | ||||
Sequence: GQYSTGKT | ||||||
Region | 230-231 | G2 motif | ||||
Sequence: EP | ||||||
Region | 292-295 | G3 motif | ||||
Sequence: DTPG | ||||||
Region | 358-361 | G4 motif | ||||
Sequence: NKAD | ||||||
Region | 382 | G5 motif | ||||
Sequence: V | ||||||
Coiled coil | 467-490 | |||||
Sequence: KAKAQQKLIDNLEDEFGKVQREHH |
Domain
The EH domain (1-93) is critical for vesicular localization.
Sequence similarities
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length545
- Mass (Da)61,230
- Last updated2001-12-01 v1
- Checksum0E5B907504D70117
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB024036 EMBL· GenBank· DDBJ | BAB02809.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002686 EMBL· GenBank· DDBJ | AEE76359.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE76360.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | ANM65913.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY034911 EMBL· GenBank· DDBJ | AAK59418.1 EMBL· GenBank· DDBJ | mRNA | ||
BT004414 EMBL· GenBank· DDBJ | AAO42408.1 EMBL· GenBank· DDBJ | mRNA | ||
AK317308 EMBL· GenBank· DDBJ | BAH19984.1 EMBL· GenBank· DDBJ | mRNA |