Q94BN2 · SPSY_ARATH
- ProteinSpermine synthase
- GeneSPMS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids359 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
Catalytic activity
- S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = H+ + S-methyl-5'-thioadenosine + spermine
Pathway
Amine and polyamine biosynthesis; spermine biosynthesis; spermine from spermidine: step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 99 | S-adenosyl 3-(methylsulfanyl)propylamine (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 129 | spermidine (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 130 | S-adenosyl 3-(methylsulfanyl)propylamine (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 154 | spermidine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 174 | S-adenosyl 3-(methylsulfanyl)propylamine (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 205-206 | S-adenosyl 3-(methylsulfanyl)propylamine (UniProtKB | ChEBI) | ||||
Sequence: DA | ||||||
Active site | 224 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 292 | putrescine (UniProtKB | ChEBI) | ||||
Sequence: Y |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | spermidine synthase activity | |
Molecular Function | spermine synthase activity | |
Biological Process | spermidine biosynthetic process | |
Biological Process | spermine biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSpermine synthase
- EC number
- Short namesSPMSY
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ94BN2
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000397673 | 1-359 | Spermine synthase | |||
Sequence: MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPHPRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPALALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFKSVHYAWSSVPTYPSGVIGFVLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVASLLAS |
Proteomic databases
Expression
Tissue specificity
Expressed predominantly in stem internodes, flower buds and roots.
Induction
Up-regulated by abscisic acid and salt stress.
Gene expression databases
Interaction
Subunit
Heterodimer. Component of a multiprotein complex. Interacts with SPDSYN1 and SPDSYN2.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q94BN2 | SPDSYN1 Q9ZUB3 | 4 | EBI-1770109, EBI-1770123 | |
BINARY | Q94BN2 | SPDSYN2 O48661 | 4 | EBI-1770109, EBI-1770100 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 53-304 | PABS | ||||
Sequence: SGWFSEPHPRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPALALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFKSVHYAWSSVPTYPSGVIGFVLCST |
Sequence similarities
Belongs to the spermidine/spermine synthase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
Q94BN2-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length359
- Mass (Da)39,239
- Last updated2001-12-01 v1
- Checksum5251D28EBB98865D
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1R7T3F8 | A0A1R7T3F8_ARATH | SPDS3 | 376 | ||
F4KJ05 | F4KJ05_ARATH | SPDS3 | 386 |
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 40 | in Ref. 4; BAH19534 | ||||
Sequence: V → E | ||||||
Sequence conflict | 204 | in Ref. 5; AAM64782 | ||||
Sequence: G → S | ||||||
Sequence conflict | 224 | in Ref. 3; AAL11565 | ||||
Sequence: D → N |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB025622 EMBL· GenBank· DDBJ | BAB08415.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002688 EMBL· GenBank· DDBJ | AED96305.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED96306.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED96307.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED96308.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED96309.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF424571 EMBL· GenBank· DDBJ | AAL11565.1 EMBL· GenBank· DDBJ | mRNA | ||
AY040013 EMBL· GenBank· DDBJ | AAK64170.1 EMBL· GenBank· DDBJ | mRNA | ||
AY079367 EMBL· GenBank· DDBJ | AAL85098.1 EMBL· GenBank· DDBJ | mRNA | ||
BT000742 EMBL· GenBank· DDBJ | AAN31883.1 EMBL· GenBank· DDBJ | mRNA | ||
AK316822 EMBL· GenBank· DDBJ | BAH19534.1 EMBL· GenBank· DDBJ | mRNA | ||
AY087226 EMBL· GenBank· DDBJ | AAM64782.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |