Q94AR8 · LEUC_ARATH

Function

function

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (PubMed:19150812, PubMed:20663849).
Functions in both the biosynthesis of leucine and in the methionine chain elongation pathway of aliphatic glucosinolate formation (PubMed:19597944).

Miscellaneous

The environment dependent reduction in IIL1 activity and severely impaired growth of cv. Bur-0 are caused by a triplet repeat expansion in the third intron of the gene (PubMed:19150812).

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site376[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site445[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site448[4Fe-4S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast
Cellular Componentchloroplast stroma
Cellular Componentplastid
Molecular Function3-isopropylmalate dehydratase activity
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionintramolecular hydroxytransferase activity
Molecular Functionmetal ion binding
Biological Processglucosinolate biosynthetic process
Biological ProcessL-leucine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3-isopropylmalate dehydratase large subunit, chloroplastic
  • EC number
  • Alternative names
    • 2-(omega-methylthio)alkylmalate dehydratase large subunit
      (EC:4.2.1.170
      ) . EC:4.2.1.170 (UniProtKB | ENZYME | Rhea)
    • AtLEUC
    • Isopropylmalate isomerase large subunit 1
      (AtIIL1
      ; IPMI LSU1
      )
    • Methylthioalkylmalate isomerase large subunit
      (MAM-IL
      )

Gene names

    • Name
      IIL1
    • ORF names
      T9E8.170
    • Ordered locus names
      At4g13430

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Bur-0
    • cv. Columbia
    • cv. Pf-0
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q94AR8
  • Secondary accessions
    • Q9T0L4

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

PTM/Processing

Features

Showing features for transit peptide, chain.

Type
IDPosition(s)Description
Transit peptide1-47Chloroplast
ChainPRO_000036693948-5093-isopropylmalate dehydratase large subunit, chloroplastic

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in roots, leaves, stems and flowers. Expressed at low levels in siliques.

Gene expression databases

Interaction

Subunit

Heterodimer of the large LEUC/IIL1 subunit and the small LEUD (SSU1, SSU2 or SSU3) subunits.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-25Disordered

Sequence similarities

Belongs to the aconitase/IPM isomerase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    509
  • Mass (Da)
    55,014
  • Last updated
    2001-12-01 v1
  • Checksum
    6CE200D838894ECC
MASVISSSPFLCKSSSKSDLGISSFPKSSQISIHRCQKKSISRKIVSVMAPQKDRSPGTTGSVKTGMTMTEKILARASEKSLVVPGDNIWVNVDVLMTHDVCGPGAFGIFKREFGEKAKVWDPEKIVVIPDHYIFTADKRANRNVDIMREHCREQNIKYFYDITDLGNFKANPDYKGVCHVALAQEGHCRPGEVLLGTDSHTCTAGAFGQFATGIGNTDAGFVLGTGKILLKVPPTMRFILDGEMPSYLQAKDLILQIIGEISVAGATYKTMEFSGTTIESLSMEERMTLCNMVVEAGGKNGVIPPDATTLNYVENRTSVPFEPVYSDGNASFVADYRFDVSKLEPVVAKPHSPDNRALARECKDVKIDRVYIGSCTGGKTEDFMAAAKLFHAAGRKVKVPTFLVPATQKVWMDVYALPVPGAGGKTCAQIFEEAGCDTPASPSCGACLGGPADTYARLNEPQVCVSTTNRNFPGRMGHKEGQIYLASPYTAAASALTGRVADPREFLQ

Sequence caution

The sequence CAB40778.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence CAB78385.1 differs from that shown. Reason: Erroneous gene model prediction

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL049608
EMBL· GenBank· DDBJ
CAB40778.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AL161536
EMBL· GenBank· DDBJ
CAB78385.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002687
EMBL· GenBank· DDBJ
AEE83278.1
EMBL· GenBank· DDBJ
Genomic DNA
AY045842
EMBL· GenBank· DDBJ
AAK76516.1
EMBL· GenBank· DDBJ
mRNA
AY117151
EMBL· GenBank· DDBJ
AAM51226.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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