Q94AR8 · LEUC_ARATH
- Protein3-isopropylmalate dehydratase large subunit, chloroplastic
- GeneIIL1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids509 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (PubMed:19150812, PubMed:20663849).
Functions in both the biosynthesis of leucine and in the methionine chain elongation pathway of aliphatic glucosinolate formation (PubMed:19597944).
Functions in both the biosynthesis of leucine and in the methionine chain elongation pathway of aliphatic glucosinolate formation (PubMed:19597944).
Miscellaneous
The environment dependent reduction in IIL1 activity and severely impaired growth of cv. Bur-0 are caused by a triplet repeat expansion in the third intron of the gene (PubMed:19150812).
Catalytic activity
- (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate
Cofactor
Note: Binds 1 [4Fe-4S] cluster per subunit.
Pathway
Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 376 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 445 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 448 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | chloroplast stroma | |
Cellular Component | plastid | |
Molecular Function | 3-isopropylmalate dehydratase activity | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | intramolecular hydroxytransferase activity | |
Molecular Function | metal ion binding | |
Biological Process | glucosinolate biosynthetic process | |
Biological Process | L-leucine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3-isopropylmalate dehydratase large subunit, chloroplastic
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ94AR8
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transit peptide | 1-47 | Chloroplast | |||
Chain | PRO_0000366939 | 48-509 | 3-isopropylmalate dehydratase large subunit, chloroplastic | ||
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in roots, leaves, stems and flowers. Expressed at low levels in siliques.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-25 | Disordered | |||
Sequence similarities
Belongs to the aconitase/IPM isomerase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length509
- Mass (Da)55,014
- Last updated2001-12-01 v1
- Checksum6CE200D838894ECC
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL049608 EMBL· GenBank· DDBJ | CAB40778.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AL161536 EMBL· GenBank· DDBJ | CAB78385.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002687 EMBL· GenBank· DDBJ | AEE83278.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY045842 EMBL· GenBank· DDBJ | AAK76516.1 EMBL· GenBank· DDBJ | mRNA | ||
AY117151 EMBL· GenBank· DDBJ | AAM51226.1 EMBL· GenBank· DDBJ | mRNA |