Q949U1 · C79F1_ARATH
- ProteinDihomomethionine N-hydroxylase
- GeneCYP79F1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids538 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the conversion of the short chain elongated methionines di-, tri-, and tetrahomomethionine to their respective aldoximes 5-methylthiopentanaldoxime, 6-methylthiohexanaldoxime, and 7-methylheptanaldoxime.
Catalytic activity
- an L-polyhomomethionine + 2 O2 + 2 reduced [NADPH--hemoprotein reductase] = an (E)-omega-(methylsulfanyl)-alkanal oxime + CO2 + 2 H+ + 3 H2O + 2 oxidized [NADPH--hemoprotein reductase]
an L-polyhomomethionine RHEA-COMP:13111 + 2 CHEBI:15379 + 2 RHEA-COMP:11964 = an (E)-ω-(methylsulfanyl)-alkanal oxime RHEA-COMP:13114 + CHEBI:16526 + 2 CHEBI:15378 + 3 CHEBI:15377 + 2 RHEA-COMP:11965
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
34 μM | dihomomethionine | |||||
37 μM | trihomomethionine | |||||
194 μM | tetrahomomethionine | |||||
216 μM | pentahomomethionine | |||||
74 μM | hexahomomethionine |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | nucleus | |
Molecular Function | CYP79F1 dihomomethionine monooxygenase activity | |
Molecular Function | CYP79F1 trihomomethionine monooxygenase activity | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen | |
Biological Process | glucosinolate biosynthetic process | |
Biological Process | response to insect |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDihomomethionine N-hydroxylase
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ949U1
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 8-28 | Helical | ||||
Sequence: LPYPFHILLVFILSMASITLL |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Plants have a bushy phenotype with crinkled leaves and retarded vascularization.
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 43 | in strain: cv. Ag-0, cv. Br-0, cv. C24, cv. Ct-1, cv. Edi-0, cv. Kas-1, cv. Kin-0, cv. Landsberg erecta, cv. Ll-0, cv. Lz-0, cv. Ms-0, cv. Mt-0, cv. Nd-1, cv. Nok-3, cv. Oy-0, cv. Sorbo, cv. Van-0, cv. Wa-1 and cv. Wassilewskija | ||||
Sequence: C → R | ||||||
Natural variant | 83 | in strain: cv. Se-0 | ||||
Sequence: A → G | ||||||
Natural variant | 120 | in strain: cv. Br-0 and cv. Mt-0 | ||||
Sequence: I → T | ||||||
Natural variant | 154 | in strain: cv. Ag-0, cv. Cvi-1, cv. Edi-0, cv. Ll-0 and cv. Nok-3 | ||||
Sequence: V → F | ||||||
Natural variant | 158 | in strain: cv. Ag-0, cv. Br-0, cv. Cvi-1, cv. Edi-0, cv. Ll-0, cv. Lz-0, cv. Mt-0, cv. Nok-3 and cv. Van-0 | ||||
Sequence: K → N | ||||||
Natural variant | 159 | in strain: cv. Nd-1 | ||||
Sequence: M → I | ||||||
Natural variant | 161 | in strain: cv. Lz-0 and cv. Van-0 | ||||
Sequence: E → K | ||||||
Natural variant | 192 | in strain: cv. Kin-0 | ||||
Sequence: R → W | ||||||
Natural variant | 222 | in strain: cv. Ll-0 | ||||
Sequence: G → V |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 46 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000315843 | 1-538 | Dihomomethionine N-hydroxylase | |||
Sequence: MMSFTTSLPYPFHILLVFILSMASITLLGRILSRPTKTKDRSCQLPPGPPGWPILGNLPELFMTRPRSKYFRLAMKELKTDIACFNFAGIRAITINSDEIAREAFRERDADLADRPQLFIMETIGDNYKSMGISPYGEQFMKMKRVITTEIMSVKTLKMLEAARTIEADNLIAYVHSMYQRSETVDVRELSRVYGYAVTMRMLFGRRHVTKENVFSDDGRLGNAEKHHLEVIFNTLNCLPSFSPADYVERWLRGWNVDGQEKRVTENCNIVRSYNNPIIDERVQLWREEGGKAAVEDWLDTFITLKDQNGKYLVTPDEIKAQCVEFCIAAIDNPANNMEWTLGEMLKNPEILRKALKELDEVVGRDRLVQESDIPNLNYLKACCRETFRIHPSAHYVPSHLARQDTTLGGYFIPKGSHIHVCRPGLGRNPKIWKDPLVYKPERHLQGDGITKEVTLVETEMRFVSFSTGRRGCIGVKVGTIMMVMLLARFLQGFNWKLHQDFGPLSLEEDDASLLMAKPLHLSVEPRLAPNLYPKFRP |
Proteomic databases
Expression
Tissue specificity
Highly expressed in cotyledons, leaves, stems and siliques. Detected in flowers and lateral roots, but not in the main root. Expressed only in the vascular bundles in apical plant parts.
Induction
By methyl jasmonate.
Gene expression databases
Structure
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q949U1-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length538
- Mass (Da)61,695
- Last updated2001-12-01 v1
- Checksum4001A1179BBE5ADD
Q949U1-2
- Name2
Sequence caution
Features
Showing features for alternative sequence.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC006341 EMBL· GenBank· DDBJ | AAD34693.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
CP002684 EMBL· GenBank· DDBJ | AEE29447.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE29448.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY035021 EMBL· GenBank· DDBJ | AAK59526.1 EMBL· GenBank· DDBJ | mRNA | ||
AY050890 EMBL· GenBank· DDBJ | AAK92827.1 EMBL· GenBank· DDBJ | mRNA | ||
AY114074 EMBL· GenBank· DDBJ | AAM45122.1 EMBL· GenBank· DDBJ | mRNA | ||
EF598753 EMBL· GenBank· DDBJ | ABR09166.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598754 EMBL· GenBank· DDBJ | ABR09167.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598755 EMBL· GenBank· DDBJ | ABR09168.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598756 EMBL· GenBank· DDBJ | ABR09169.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598757 EMBL· GenBank· DDBJ | ABR09170.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598758 EMBL· GenBank· DDBJ | ABR09171.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598759 EMBL· GenBank· DDBJ | ABR09172.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598760 EMBL· GenBank· DDBJ | ABR09173.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598761 EMBL· GenBank· DDBJ | ABR09174.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598762 EMBL· GenBank· DDBJ | ABR09175.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598763 EMBL· GenBank· DDBJ | ABR09176.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598764 EMBL· GenBank· DDBJ | ABR09177.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598765 EMBL· GenBank· DDBJ | ABR09178.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598766 EMBL· GenBank· DDBJ | ABR09179.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598767 EMBL· GenBank· DDBJ | ABR09180.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598768 EMBL· GenBank· DDBJ | ABR09181.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598769 EMBL· GenBank· DDBJ | ABR09182.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598770 EMBL· GenBank· DDBJ | ABR09183.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598771 EMBL· GenBank· DDBJ | ABR09184.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598772 EMBL· GenBank· DDBJ | ABR09185.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598773 EMBL· GenBank· DDBJ | ABR09186.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598774 EMBL· GenBank· DDBJ | ABR09187.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598775 EMBL· GenBank· DDBJ | ABR09188.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF598776 EMBL· GenBank· DDBJ | ABR09189.1 EMBL· GenBank· DDBJ | Genomic DNA |