Q949N0 · NAC53_ARATH
- ProteinNAC domain-containing protein 53
- GeneNAC053
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids549 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Transcriptional activator activated by proteolytic cleavage through regulated intramembrane proteolysis (RIP) (PubMed:24323506, PubMed:25219309, Ref.6). Promotes reactive oxygen species (ROS) production during drought-induced leaf senescence. In response to abscisic acid (ABA)-mediated drought stress signals, binds directly to the promoters of RBOHC and RBOHE genes, encoding ROS biosynthetic enzymes, resulting in ROS accumulation and triggering leaf senescence via programmed cell death (PCD). ROS-induced leaf senescence sustains plant survival under drought conditions (PubMed:22313226).
Involved in heat stress response. Modulates PCD through a ROS-mediated positive feedback control under heat stress conditions. This may provide an adaptation strategy for plant survival under extreme heat stress conditions (PubMed:25219309).
Acts as a repressor in preventing anther dehiscence during stamen development by suppressing genes that participate in jasmonic acid (JA) biosynthesis, such as DAD1, AOS, AOC3, OPR3 and 4CLL5/OPCL1 (PubMed:24323506).
Involved in heat stress response. Modulates PCD through a ROS-mediated positive feedback control under heat stress conditions. This may provide an adaptation strategy for plant survival under extreme heat stress conditions (PubMed:25219309).
Acts as a repressor in preventing anther dehiscence during stamen development by suppressing genes that participate in jasmonic acid (JA) biosynthesis, such as DAD1, AOS, AOC3, OPR3 and 4CLL5/OPCL1 (PubMed:24323506).
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
DNA binding | 108-165 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | nucleus | |
Molecular Function | DNA-binding transcription factor activity | |
Molecular Function | transcription cis-regulatory region binding | |
Biological Process | drought recovery | |
Biological Process | leaf senescence | |
Biological Process | positive regulation of DNA-templated transcription | |
Biological Process | regulation of reactive oxygen species metabolic process |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameNAC domain-containing protein 53
- Short namesANAC053
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ949N0
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass membrane protein
Note: Localized primarily in plasma membrane or endoplasmic reticulum membrane as dormant form and, upon abiotic stress, is processed into a transcriptionally active and nuclear form after a proteolytic cleavage through regulated intramembrane proteolysis (RIP).
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 526-546 | Helical | |||
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Delayed leaf senescence and enhanced drought resistance (PubMed:22313226).
Enhanced heat resistance (PubMed:25219309).
Enhanced heat resistance (PubMed:25219309).
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 44 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000432444 | 1-549 | NAC domain-containing protein 53 | ||
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in roots, rosette leaves, cauline leaves, shoot apex and stems.
Induction
By abscisic acid (ABA) (PubMed:22313226, PubMed:25219309).
Induced by heat shock (PubMed:25219309).
Induced by cold, drought stress and methyl methanesulfonate (MMS) treatment (PubMed:17158162).
Induced by heat shock (PubMed:25219309).
Induced by cold, drought stress and methyl methanesulfonate (MMS) treatment (PubMed:17158162).
Developmental stage
Expressed in the anthers and the upper parts of the stamen filaments after stage 7 to 9 of flower budding. In stage 10 flower buds, expressed in the anthers and pollen grains. Weakly expressed in mature flowers at stage 12.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 9-159 | NAC | |||
Region | 395-418 | Disordered | |||
Domain
The NAC domain includes a DNA binding domain and a dimerization domain.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length549
- Mass (Da)62,356
- Last updated2001-12-01 v1
- MD5 Checksum8BD010ED9BDACD4D33BC22DC222D7206
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1I9LN95 | A0A1I9LN95_ARATH | NAC053 | 415 |
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 74 | in Ref. 4; AAM65338 | |||
Sequence conflict | 276 | in Ref. 4; AAM65338 | |||
Sequence conflict | 297 | in Ref. 4; AAM65338 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC011560 EMBL· GenBank· DDBJ | AAG51388.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AC013428 EMBL· GenBank· DDBJ | AAF76351.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002686 EMBL· GenBank· DDBJ | AEE74917.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY051003 EMBL· GenBank· DDBJ | AAK93680.1 EMBL· GenBank· DDBJ | mRNA | ||
AY079345 EMBL· GenBank· DDBJ | AAL85076.1 EMBL· GenBank· DDBJ | mRNA | ||
AY087802 EMBL· GenBank· DDBJ | AAM65338.1 EMBL· GenBank· DDBJ | mRNA |