Essential maintenance is planned to begin on Fri Jan 24 2025. The website may be temporarily unavailable. Please use our fallback: https://wwwdev.ebi.ac.uk/uniprot/front-end/fallback/ in case of any outage.

Q94734 · NP4_RHOPR

Function

function

Heme-based protein that delivers nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also binds tightly to histamine, which is released by the host to induce wound healing. NO release is pH dependent and linked to loop dynamics.

Miscellaneous

Superposition of the structures of NP4 in the presence of ammonia or nitrite shows a conformational change of the protein upon NO2- binding.

Catalytic activity

Cofactor

heme b (UniProtKB | Rhea| CHEBI:60344 )

Note: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site80Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); proximal binding residue

GO annotations

AspectTerm
Cellular Componentextracellular region
Molecular Functionhistamine binding
Molecular Functionmetal ion binding
Molecular Functionnitric oxide binding
Molecular Functionoxidoreductase activity
Biological Processvasodilation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nitrophorin-4
  • EC number
  • Short names
    NP4
  • Alternative names
    • Nitrite dismutase

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Paraneoptera > Hemiptera > Heteroptera > Panheteroptera > Cimicomorpha > Reduviidae > Triatominae > Rhodnius

Accessions

  • Primary accession
    Q94734

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis51Loss of NO-induced conformational change and strongly reduced pH dependence for NO release. Stabilization of the ferric-NO heme center and decreased nitrite disproportionation.
Mutagenesis76Stabilization of the ferric-NO heme center.
Mutagenesis142No effect on NO-induced conformational change or on pH dependence for NO release. Increased NO association rates.
Mutagenesis150-151Loss of NO-induced conformational change and loss of pH dependence for NO release.
Mutagenesis151Coordination of the heme iron center and inhibition of nitrite disproportionation.

PTM/Processing

Features

Showing features for signal, chain, disulfide bond.

Type
IDPosition(s)Description
Signal1-21
ChainPRO_000002182622-205Nitrophorin-4
Disulfide bond23↔143
Disulfide bond62↔192

Keywords

Expression

Tissue specificity

Expressed by the salivary glands.

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the calycin superfamily. Nitrophorin family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    205
  • Mass (Da)
    22,406
  • Last updated
    1997-02-01 v1
  • MD5 Checksum
    F5977553120DACD987D302FA0A1656E7
MKSYTSLLAVAILCLFGGVNGACTKNAIAQTGFNKDKYFNGDVWYVTDYLDLEPDDVPKRYCAALAAGTASGKLKEALYHYDPKTQDTFYDVSELQVESLGKYTANFKKVDKNGNVKVAVTAGNYYTFTVMYADDSSALIHTCLHKGNKDLGDLYAVLNRNKDAAAGDKVKSAVSAATLEFSKFISTKENNCAYDNDSLKSLLTK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U70584
EMBL· GenBank· DDBJ
AAB09590.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help