Q94515 · DEGS1_DROME
- ProteinSphingolipid delta(4)-desaturase DES1
- Geneifc
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids321 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Has sphingolipid-delta-4-desaturase activity. Converts sphinganine-containing sphingolipids (such as N-acylsphinganines or dihydroceramides) into sphingolipids containing the delta-4-desaturated sphingoid base (E)-sphing-4-enine (such as N-acylsphing-4-enines or ceramides), which are required for many different functions (structural functions as well as signaling) (PubMed:11937514).
Required to initiate spermatid differentiation among other signals (PubMed:9003299).
Required for central spindle assembly and cytokinesis during male meiosis, may act as part of an anchoring mechanism that links membrane-bounded cellular compartments to components of the cytoskeleton (PubMed:9819060).
Required to initiate spermatid differentiation among other signals (PubMed:9003299).
Required for central spindle assembly and cytokinesis during male meiosis, may act as part of an anchoring mechanism that links membrane-bounded cellular compartments to components of the cytoskeleton (PubMed:9819060).
Catalytic activity
- an N-acylsphinganine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H+ = an N-acylsphing-4-enine + 2 Fe(III)-[cytochrome b5] + 2 H2OThis reaction proceeds in the forward direction.
- an N-acyleicosasphinganine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H+ = an N-acyleicosasphing-4-enine + 2 Fe(III)-[cytochrome b5] + 2 H2OThis reaction proceeds in the forward direction.
Pathway
Sphingolipid metabolism.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | late endosome | |
Cellular Component | mitochondrion | |
Molecular Function | sphingolipid delta-4 desaturase activity | |
Biological Process | ceramide biosynthetic process | |
Biological Process | negative regulation of lipophagy |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameSphingolipid delta(4)-desaturase DES1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ94515
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 41-61 | Helical | |||
Transmembrane | 68-88 | Helical | |||
Transmembrane | 107-127 | Helical | |||
Transmembrane | 157-177 | Helical | |||
Transmembrane | 187-206 | Helical | |||
Transmembrane | 208-230 | Helical | |||
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Primary spermatocytes become mature in size but degenerate without initiating meiotic chromosome condensation in their nuclei. The mutation does not affect female fertility but leads to semi-lethality both in males and females during embryonic stages (PubMed:9003299).
Homozigous mutant spermatids show a misshapen Nebenkern, which varies in size and is associated with multiple nuclei (PubMed:9819060).
Homozigous mutant spermatids show a misshapen Nebenkern, which varies in size and is associated with multiple nuclei (PubMed:9819060).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000453621 | 1-321 | Sphingolipid delta4-desaturase DES1 | ||
Proteomic databases
Expression
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length321
- Mass (Da)37,213
- Last updated1997-02-01 v1
- MD5 ChecksumD1B931025699BB5B2EF17D12C141887D
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
M9PC47 | M9PC47_DROME | ifc | 102 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE014134 EMBL· GenBank· DDBJ | AAF52318.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X94180 EMBL· GenBank· DDBJ | CAA63889.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY061196 EMBL· GenBank· DDBJ | AAL28744.1 EMBL· GenBank· DDBJ | mRNA | ||
AF466379 EMBL· GenBank· DDBJ | AAM12535.1 EMBL· GenBank· DDBJ | mRNA |