Q93ZB2 · KO1_ARATH

Function

function

Catalyzes three successive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid, a key step in gibberellins (GAs) biosynthesis. GAs, which are involved many processes, including stem elongation, play a central role in plant development.

Catalytic activity

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
1.8 μMent-kaurene
6 μMent-kaurenol
1.9 μMent-kaurenal
10 μMent-isokaurene
28 μMent-beyerene

Pathway

Plant hormone biosynthesis; gibberellin biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site454Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentchloroplast outer membrane
Cellular Componentendoplasmic reticulum
Molecular Functionent-kaur-16-en-19-al oxidase activity
Molecular Functionent-kaur-16-en-19-ol oxidase activity
Molecular Functionent-kaurene oxidase activity
Molecular Functionheme binding
Molecular Functioniron ion binding
Biological Processent-kaurene oxidation to kaurenoic acid
Biological Processgibberellic acid mediated signaling pathway
Biological Processgibberellin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ent-kaurene oxidase, chloroplastic
  • EC number
  • Short names
    AtKO1
  • Alternative names
    • Cytochrome P450 701A3

Gene names

    • Name
      KO
    • Synonyms
      CYP701A3, GA3, KO1
    • ORF names
      T1N24.23
    • Ordered locus names
      At5g25900

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Columbia
    • cv. Landsberg erecta
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q93ZB2
  • Secondary accessions
    • O81188
    • Q9S7C8

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-2Chloroplast intermembrane
Transmembrane3-23Helical
Topological domain24-509Cytoplasmic

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000521811-509Ent-kaurene oxidase, chloroplastic

Proteomic databases

PTM databases

Expression

Tissue specificity

Widely expressed. Highly expressed in influorescence tissues. In germinating seeds, it is mainly localized in the cortex and endodermis of embryo axis.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    509
  • Mass (Da)
    58,160
  • Last updated
    2004-07-19 v2
  • Checksum
    A84F7E2C78F0E83F
MAFFSMISILLGFVISSFIFIFFFKKLLSFSRKNMSEVSTLPSVPVVPGFPVIGNLLQLKEKKPHKTFTRWSEIYGPIYSIKMGSSSLIVLNSTETAKEAMVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQKRKRHYRDALIENVSSKLHAHARDHPQEPVNFRAIFEHELFGVALKQAFGKDVESIYVKELGVTLSKDEIFKVLVHDMMEGAIDVDWRDFFPYLKWIPNKSFEARIQQKHKRRLAVMNALIQDRLKQNGSESDDDCYLNFLMSEAKTLTKEQIAILVWETIIETADTTLVTTEWAIYELAKHPSVQDRLCKEIQNVCGGEKFKEEQLSQVPYLNGVFHETLRKYSPAPLVPIRYAHEDTQIGGYHVPAGSEIAINIYGCNMDKKRWERPEDWWPERFLDDGKYETSDLHKTMAFGAGKRVCAGALQASLMAGIAIGRLVQEFEWKLRDGEEENVDTYGLTSQKLYPLMAIINPRRS

Sequence caution

The sequence AAL15302.1 differs from that shown. Reason: Frameshift

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict97in Ref. 1; AAC39507

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF047719
EMBL· GenBank· DDBJ
AAC39505.1
EMBL· GenBank· DDBJ
mRNA
AF047720
EMBL· GenBank· DDBJ
AAC39506.1
EMBL· GenBank· DDBJ
Genomic DNA
AF047721
EMBL· GenBank· DDBJ
AAC39507.1
EMBL· GenBank· DDBJ
Genomic DNA
AF149413
EMBL· GenBank· DDBJ
AAD40137.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED93499.1
EMBL· GenBank· DDBJ
Genomic DNA
AY057671
EMBL· GenBank· DDBJ
AAL15302.1
EMBL· GenBank· DDBJ
mRNA Frameshift

Genome annotation databases

Similar Proteins

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