Q93HS4 · RTXC_BRAEL
- ProteinDihydrorhizobitoxine desaturase
- GenertxC
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids352 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Involved in the biosynthesis of the nodulation enhancer compound rhizobitoxine. Catalyzes the final step of the pathway, the introduction of a carbon double bond into the C3 position of dihydrorhizobitoxine to produce rhizobitoxine.
Catalytic activity
- dihydrorhizobitoxine + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H+ = rhizobitoxine + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 H2OThis reaction proceeds in the forward direction.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water | |
Biological Process | lipid metabolic process |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDihydrorhizobitoxine desaturase
- EC number
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Nitrobacteraceae > Bradyrhizobium
Accessions
- Primary accessionQ93HS4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 53-73 | Helical | ||||
Sequence: LATLCVSWWLYPLAVLLIGAY | ||||||
Transmembrane | 89-109 | Helical | ||||
Sequence: LAKNTTWNYVLGILFAAYPLF | ||||||
Transmembrane | 204-224 | Helical | ||||
Sequence: IGILAGSYALGLLHIVILFWI |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Mutant can produce serinol and dihydrorhizobitoxine, but not rhizobitoxine (PubMed:11679318, PubMed:14711685).
Mutant produces no symptoms of foliar chlorosis on G. max cv. Lee and does not accumulate rhizobitoxine in the nodules or upper shoots (PubMed:14711685).
It shows significantly less nodulation competitiveness than the wild-type strain on M. atropurpureum (PubMed:14711685).
Mutant produces no symptoms of foliar chlorosis on G. max cv. Lee and does not accumulate rhizobitoxine in the nodules or upper shoots (PubMed:14711685).
It shows significantly less nodulation competitiveness than the wild-type strain on M. atropurpureum (PubMed:14711685).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000454506 | 1-352 | Dihydrorhizobitoxine desaturase | |||
Sequence: MNQADAWKLRSSRYEAVQFSREINKQLSELRPDNVMGAIYIAKDYAVIAACTLATLCVSWWLYPLAVLLIGAYQRGLTTIAHDAAHRTLAKNTTWNYVLGILFAAYPLFQRHWAYRISHVYLHHPYLGDPEKDPDLKFFMANGVYDVQPPKRYAFNIIWKPIFGGATLAYLKYLWTNRFSITDSEDQSRSSILVDKYGFYLFWIGILAGSYALGLLHIVILFWIVPYLTTFQVLGWFVELAEHSPMCESETKNVYLTRNRKGNFLERAILGQNLDEYHLEHHLSPGIPFWLLHKAQKIRMQDPGYAKVAASWGGLFVKGPQGQPSVITQLKERNRRLYEQSLADAHAKGHVA |
Structure
Sequence
- Sequence statusComplete
- Length352
- Mass (Da)40,461
- Last updated2001-12-01 v1
- Checksum7C4F6FE5F9DC19A3
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB062279 EMBL· GenBank· DDBJ | BAB55901.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BJNL01000074 EMBL· GenBank· DDBJ | GEC57952.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
SWAO01000075 EMBL· GenBank· DDBJ | NWL71962.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JAFICZ010000001 EMBL· GenBank· DDBJ | MBP1299571.1 EMBL· GenBank· DDBJ | Genomic DNA |