Q93086 · P2RX5_HUMAN
- ProteinP2X purinoceptor 5
- GeneP2RX5
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids444 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Isoform 6
ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium and calcium (PubMed:12761352).
Unlike, other P2RX receptors, the P2X5 receptor is also permeable to chloride (PubMed:12761352).
May play a supporting role in the inflammatory response (By similarity).
Unlike, other P2RX receptors, the P2X5 receptor is also permeable to chloride (PubMed:12761352).
May play a supporting role in the inflammatory response (By similarity).
Isoform 1
Non-functional.
Catalytic activity
- Na+(in) = Na+(out)
- Ca2+(in) = Ca2+(out)
- chloride(in) = chloride(out)
Activity regulation
Activated by ATP (PubMed:12761352).
Slowly desensitizing (PubMed:12761352).
Sensitives to the ATP agonist:alpha/beta-methylene-ATP (PubMed:12761352).
Slowly desensitizing (PubMed:12761352).
Sensitives to the ATP agonist:alpha/beta-methylene-ATP (PubMed:12761352).
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | plasma membrane | |
Cellular Component | postsynapse | |
Molecular Function | ATP binding | |
Molecular Function | ATP-gated ion channel activity | |
Molecular Function | extracellularly ATP-gated monoatomic cation channel activity | |
Molecular Function | ligand-gated monoatomic anion channel activity | |
Molecular Function | monoatomic ion channel activity | |
Molecular Function | purinergic nucleotide receptor activity | |
Molecular Function | transmembrane signaling receptor activity | |
Biological Process | calcium ion transmembrane transport | |
Biological Process | chloride transport | |
Biological Process | nervous system development | |
Biological Process | osteoclast maturation | |
Biological Process | positive regulation of calcium ion transport into cytosol | |
Biological Process | positive regulation of calcium-mediated signaling | |
Biological Process | response to ATP | |
Biological Process | signal transduction |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameP2X purinoceptor 5
- Short namesP2X5
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ93086
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Isoform 6
Cell membrane ; Multi-pass membrane protein
Note: The absence of the exon 10-encoded amino acids substantially results in subunit aggregation and retention in the cell cytoplasm.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-30 | Cytoplasmic | ||||
Sequence: MGQAGCKGLCLSLFDYKTEKYVIAKNKKVG | ||||||
Transmembrane | 31-51 | Helical; Name=1 | ||||
Sequence: LLYRLLQASILAYLVVWVFLI | ||||||
Topological domain | 52-319 | Extracellular | ||||
Sequence: KKGYQDVDTSLQSAVITKVKGVAFTNTSDLGQRIWDVADYVIPAQGENVFFVVTNLIVTPNQRQNVCAENEGIPDGACSKDSDCHAGEAVTAGNGVKTGRCLRRENLARGTCEIFAWCPLETSSRPEEPFLKEAEDFTIFIKNHIRFPKFNFSKSNVMDVKDRSFLKSCHFGPKNHYCPIFRLGSVIRWAGSDFQDIALEGGVIGINIEWNCDLDKAASECHPHYSFSRLDNKLSKSVSSGYNFRFARYYRDAAGVEFRTLMKAYGIR | ||||||
Transmembrane | 320-362 | Helical; Name=2 | ||||
Sequence: FDVMVNGKAGKFSIIPTIINVGSGVALMGAGAFFCDLVLIYLI | ||||||
Topological domain | 363-444 | Cytoplasmic | ||||
Sequence: KKREFYRDKKYEEVRGLEDSSQEAEDEASGLGLSEQLTSGPGLLGMPEQQELQEPPEAKRGSSSQKGNGSVCPQLLEPHRST |
Keywords
- Cellular component
Disease & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 355 | Impairs homotrimerization. | ||||
Sequence: D → L |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 541 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for chain, glycosylation, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000161555 | 1-444 | P2X purinoceptor 5 | |||
Sequence: MGQAGCKGLCLSLFDYKTEKYVIAKNKKVGLLYRLLQASILAYLVVWVFLIKKGYQDVDTSLQSAVITKVKGVAFTNTSDLGQRIWDVADYVIPAQGENVFFVVTNLIVTPNQRQNVCAENEGIPDGACSKDSDCHAGEAVTAGNGVKTGRCLRRENLARGTCEIFAWCPLETSSRPEEPFLKEAEDFTIFIKNHIRFPKFNFSKSNVMDVKDRSFLKSCHFGPKNHYCPIFRLGSVIRWAGSDFQDIALEGGVIGINIEWNCDLDKAASECHPHYSFSRLDNKLSKSVSSGYNFRFARYYRDAAGVEFRTLMKAYGIRFDVMVNGKAGKFSIIPTIINVGSGVALMGAGAFFCDLVLIYLIKKREFYRDKKYEEVRGLEDSSQEAEDEASGLGLSEQLTSGPGLLGMPEQQELQEPPEAKRGSSSQKGNGSVCPQLLEPHRST | ||||||
Glycosylation | 77 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 118↔169 | |||||
Sequence: CAENEGIPDGACSKDSDCHAGEAVTAGNGVKTGRCLRRENLARGTCEIFAWC | ||||||
Disulfide bond | 129↔152 | |||||
Sequence: CSKDSDCHAGEAVTAGNGVKTGRC | ||||||
Disulfide bond | 135↔163 | |||||
Sequence: CHAGEAVTAGNGVKTGRCLRRENLARGTC | ||||||
Glycosylation | 202 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 220↔229 | |||||
Sequence: CHFGPKNHYC | ||||||
Disulfide bond | 263↔272 | |||||
Sequence: CDLDKAASEC |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed at high levels in brain and immune system.
Gene expression databases
Organism-specific databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 401-444 | Disordered | ||||
Sequence: SGPGLLGMPEQQELQEPPEAKRGSSSQKGNGSVCPQLLEPHRST | ||||||
Compositional bias | 418-444 | Polar residues | ||||
Sequence: PEAKRGSSSQKGNGSVCPQLLEPHRST |
Domain
The second transmembrane domain and the conserved Asp-355 are essential for P2RX5 subunit assembly.
Sequence similarities
Belongs to the P2X receptor family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 6 isoforms produced by Alternative splicing.
Q93086-6
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name6
- Length444
- Mass (Da)49,303
- Last updated2024-05-29 v5
- ChecksumB7EF070C741C1B00
Q93086-3
- Name1
- NoteDue to exon 10 skipping this isoform is trimerization-defective and therefore enable of assembling into a functional ATP-gated receptor channel.
- Differences from canonical
- 328-349: Missing
Q93086-1
- Name2
- SynonymsA
Q93086-2
- Name3
- SynonymsB
Q93086-4
- Name4
Q93086-5
- Name5
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Sequence caution
Features
Showing features for sequence conflict, alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 96 | in Ref. 1; AAB08577 | ||||
Sequence: Q → E | ||||||
Sequence conflict | 97-98 | in Ref. 1; AAB08576 | ||||
Sequence: GE → EK | ||||||
Alternative sequence | VSP_062292 | 97-120 | in isoform 3 and isoform 5 | |||
Sequence: Missing | ||||||
Sequence conflict | 156 | in Ref. 2; AAC51931 | ||||
Sequence: E → G | ||||||
Alternative sequence | VSP_062293 | 205-206 | in isoform 3 and isoform 2 | |||
Sequence: KS → N | ||||||
Sequence conflict | 206 | in Ref. 2; AAC51931 | ||||
Sequence: S → N | ||||||
Sequence conflict | 237-238 | in Ref. 2; AAC51931 | ||||
Sequence: VI → IV | ||||||
Sequence conflict | 251 | in Ref. 2; AAC51931 | ||||
Sequence: E → R | ||||||
Alternative sequence | VSP_062294 | 328-349 | in isoform 1, isoform 2, isoform 3, isoform 4 and isoform 5 | |||
Sequence: Missing | ||||||
Sequence conflict | 352 | in Ref. 1; AAB08576/AAB08577 | ||||
Sequence: F → S | ||||||
Sequence conflict | 372 | in Ref. 5; ABB29978 | ||||
Sequence: K → R | ||||||
Sequence conflict | 374 | in Ref. 1; AAB08576/AAB08577 | ||||
Sequence: E → Q | ||||||
Compositional bias | 418-444 | Polar residues | ||||
Sequence: PEAKRGSSSQKGNGSVCPQLLEPHRST | ||||||
Sequence conflict | 421-422 | in Ref. 1; AAB08576/AAB08577 | ||||
Sequence: KR → NV | ||||||
Alternative sequence | VSP_062295 | 443-444 | in isoform 4 | |||
Sequence: ST → LRTPSASPLHQE |
Polymorphism
A thymine (T) to guanine (G) nucleotide substitution at genomic position chr17:3688012 is very common in human populations. This SNP affects P2RX5 exon 10 splicing resulting in an apparently non-functional protein. This protein lacks the 22 amino acids encoded by exon 10, including parts of the ATP-binding domain and the second transmembrane domain, and is trimerization-defective. The sequence shown in this entry corresponds to the rarer, full length isoform (isoform 6) which is capable of assembling into a functional ATP-gated receptor channel.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U49395 EMBL· GenBank· DDBJ | AAB08576.1 EMBL· GenBank· DDBJ | mRNA | ||
U49396 EMBL· GenBank· DDBJ | AAB08577.1 EMBL· GenBank· DDBJ | mRNA | ||
AF016709 EMBL· GenBank· DDBJ | AAC51931.1 EMBL· GenBank· DDBJ | mRNA | ||
AF168787 EMBL· GenBank· DDBJ | AAF43105.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF168787 EMBL· GenBank· DDBJ | AAF43106.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AF070573 EMBL· GenBank· DDBJ | AAC28645.1 EMBL· GenBank· DDBJ | mRNA | ||
DQ234349 EMBL· GenBank· DDBJ | ABB29978.1 EMBL· GenBank· DDBJ | mRNA | ||
AK290889 EMBL· GenBank· DDBJ | BAF83578.1 EMBL· GenBank· DDBJ | mRNA | ||
AK307959 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
AB209623 EMBL· GenBank· DDBJ | BAD92860.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AC132942 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
KF456229 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH471108 EMBL· GenBank· DDBJ | EAW90483.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC028084 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
BC039015 EMBL· GenBank· DDBJ | AAH39015.1 EMBL· GenBank· DDBJ | mRNA |