Q92Z29 · NIRK_RHIME
- ProteinCopper-containing nitrite reductase
- GenenirK
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids376 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
Catalytic activity
- Fe(III)-[cytochrome c] + H2O + nitric oxide = Fe(II)-[cytochrome c] + 2 H+ + nitrite
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Cu2+ ion. The Cu2+ ion is held by residues from each of 2 monomers of the trimer. Nitrite is bound to the Cu2+ ion site. Pseudoazurin is the physiological electron donor for the Cu-NIR in vitro.
Note: Binds 1 Cu+ ion. The Cu+ ion is bound within a single monomer.
Pathway
Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 2/4.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 131 | Cu cation 1 (UniProtKB | ChEBI); type 1 copper site | ||||
Sequence: H | ||||||
Binding site | 136 | Cu cation 2 (UniProtKB | ChEBI); type 2 copper site | ||||
Sequence: H | ||||||
Binding site | 171 | Cu cation 2 (UniProtKB | ChEBI); type 2 copper site | ||||
Sequence: H | ||||||
Binding site | 172 | Cu cation 1 (UniProtKB | ChEBI); type 1 copper site | ||||
Sequence: C | ||||||
Binding site | 181 | Cu cation 1 (UniProtKB | ChEBI); type 1 copper site | ||||
Sequence: H | ||||||
Binding site | 186 | Cu cation 1 (UniProtKB | ChEBI); type 1 copper site | ||||
Sequence: M | ||||||
Binding site | 342 | Cu cation 2 (UniProtKB | ChEBI); type 2 copper site | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | periplasmic space | |
Molecular Function | copper ion binding | |
Molecular Function | nitrite reductase (NO-forming) activity | |
Biological Process | denitrification pathway | |
Biological Process | nitrate assimilation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCopper-containing nitrite reductase
- EC number
- Alternative names
Gene names
Encoded on
- Plasmid pSymA (megaplasmid 1)
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Rhizobiaceae > Sinorhizobium/Ensifer group > Sinorhizobium
Accessions
- Primary accessionQ92Z29
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-33 | Tat-type signal | ||||
Sequence: MSEQFQMTRRSMLAGAAIAGAVTPLIGAVSAHA | ||||||
Chain | PRO_0000002991 | 34-376 | Copper-containing nitrite reductase | |||
Sequence: EEAVAKTAHINVASLPRVKVDLVKPPFVHAHTQKAEGGPKVVEFTLTIEEKKIVIDEQGTELHAMTFNGSVPGPLMVVHQDDYVELTLINPDTNTLQHNIDFHSATGALGGGALTVVNPGDTTVLRFKASKAGVFVYHCAPPGMVPWHVTSGMNGAIMVLPREGLTDGKGNSITYDKVYYVGEQDFYVPRDANGKFKKYESVGEAYADTLEVMRTLTPSHIVFNGAVGALTGDSALKAAVGEKVLIVHSQANRDTRPHLIGGHGDYVWATGKFRNAPDVDQETWFIPGGTAGAAFYTFEQPGIYAYVNHNLIEAFELGAAAHFAVTGDWNDDLMTSVRAPSGT |
Post-translational modification
Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Interaction
Subunit
Homotrimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 98-193 | Plastocyanin-like 1 | ||||
Sequence: MTFNGSVPGPLMVVHQDDYVELTLINPDTNTLQHNIDFHSATGALGGGALTVVNPGDTTVLRFKASKAGVFVYHCAPPGMVPWHVTSGMNGAIMVL | ||||||
Domain | 258-359 | Plastocyanin-like 2 | ||||
Sequence: GAVGALTGDSALKAAVGEKVLIVHSQANRDTRPHLIGGHGDYVWATGKFRNAPDVDQETWFIPGGTAGAAFYTFEQPGIYAYVNHNLIEAFELGAAAHFAVT |
Domain
The type I copper site in NIR plays a crucial role for electron transfer from pseudoazurin to the type II copper site of NIR, which comprises the catalytic center of NIR for the reduction of nitrite.
Sequence similarities
Belongs to the multicopper oxidase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length376
- Mass (Da)40,259
- Last updated2001-12-01 v1
- Checksum9B7999273001DC63
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE006469 EMBL· GenBank· DDBJ | AAK65339.1 EMBL· GenBank· DDBJ | Genomic DNA |