Q924S7 · SPRE2_MOUSE

  • Protein
    Sprouty-related, EVH1 domain-containing protein 2
  • Gene
    Spred2
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Negatively regulates Ras signaling pathways and downstream activation of MAP kinases (PubMed:11493923).
Recruits and translocates NF1 to the cell membrane, thereby enabling NF1-dependent hydrolysis of active GTP-bound Ras to inactive GDP-bound Ras (By similarity).
Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2 (PubMed:29501879).
Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells (PubMed:25576668).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasmic vesicle
Cellular Componentplasma membrane
Cellular Componenttransport vesicle membrane
Molecular Functionprotein kinase binding
Molecular Functionprotein serine/threonine kinase inhibitor activity
Molecular Functionstem cell factor receptor binding
Biological Processnegative regulation of epithelial to mesenchymal transition
Biological Processnegative regulation of ERK1 and ERK2 cascade
Biological Processnegative regulation of lens fiber cell differentiation
Biological Processnegative regulation of MAPK cascade
Biological Processnegative regulation of peptidyl-threonine phosphorylation
Biological Processnegative regulation of transforming growth factor beta receptor signaling pathway
Biological Processpositive regulation of DNA damage response, signal transduction by p53 class mediator
Biological Processregulation of protein deacetylation

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Sprouty-related, EVH1 domain-containing protein 2
  • Short names
    Spred-2

Gene names

    • Name
      Spred2

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q924S7

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Peripheral membrane protein
Cytoplasmic vesicle, secretory vesicle membrane
; Peripheral membrane protein
Cytoplasm
Note: Detected in the cytoplasm of the stratum spinosum cells, where it is associated with cytoplasmic vesicles that are supposed to be secretory granules.

Keywords

Phenotypes & Variants

Disruption phenotype

SPRED2 knockout results in a dwarf phenotype characterized by reduced growth and body weight, shorter tibia length, and narrower growth plates as compared with wild-type mice. Mutant animals regularly develop kyphosis and scoliosis, and show craniofacial defects, splenomegaly, and cardiac hypertrophy with arrhythmias.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 27 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000769111-410Sprouty-related, EVH1 domain-containing protein 2
Modified residue224Phosphotyrosine
Modified residue227Phosphotyrosine

Post-translational modification

Phosphorylated on serine and threonine residues. Phosphorylated on tyrosine. Phosphorylation of Tyr-224 and Tyr-227 are required for ubiquitination.
Ubiquitinated; leading to degradation by the proteasome.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Predominantly expressed in lung, liver and testis. In testis, it is specially found in mature spermatids projecting into the lumen of the seminiferous. Strongly expressed in glandular epithelia. Also expressed in embryonic tissues such as heart, lung, liver and brain.

Gene expression databases

Interaction

Subunit

Homodimer and heterodimer (By similarity).
Able to interact with SPRED1 to form heterodimers (By similarity).
Interacts with RAS (PubMed:11493923).
May interact with ZDHHC13 (via ANK repeats) and ZDHHC17 (via ANK repeats) (PubMed:28882895).
Interacts with TESK1 (PubMed:17974561).
Interacts with NF1 (By similarity).

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain5-122WH1
Region127-171Disordered
Compositional bias145-171Polar residues
Domain197-252KBD
Region274-294Disordered
Domain300-408SPR

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    410
  • Mass (Da)
    46,794
  • Last updated
    2001-12-01 v1
  • Checksum
    8A652D06CE8D3BB1
MTEETHPDDDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIHGERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIEDLIEGSTTSSSTLHNEAELGDDDVFTTATDSSSNSSQKREPTTRTISSPTSCEHRKIYTLDPYPMDHYHPDQRLPRSYPQVTFPEDDEEIVRINPREKIWMTGYEDYRHAPVRGKYLDTTEDADSYVRFAKGEVPKHEYTYPYVDSSDFGFGEDPKGSVIKTQPPRAKSRRRKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACHRCGVMCRCCGGKHKAAA

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0AAQ4VMS6A0AAQ4VMS6_MOUSESpred2407
Q5SRF8Q5SRF8_MOUSESpred2437
Q5SRF9Q5SRF9_MOUSESpred2229

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias145-171Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB063496
EMBL· GenBank· DDBJ
BAB62849.1
EMBL· GenBank· DDBJ
mRNA
AK036430
EMBL· GenBank· DDBJ
BAC29425.1
EMBL· GenBank· DDBJ
mRNA
BC066013
EMBL· GenBank· DDBJ
AAH66013.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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