Q924S7 · SPRE2_MOUSE
- ProteinSprouty-related, EVH1 domain-containing protein 2
- GeneSpred2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids410 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Negatively regulates Ras signaling pathways and downstream activation of MAP kinases (PubMed:11493923).
Recruits and translocates NF1 to the cell membrane, thereby enabling NF1-dependent hydrolysis of active GTP-bound Ras to inactive GDP-bound Ras (By similarity).
Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2 (PubMed:29501879).
Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells (PubMed:25576668).
Recruits and translocates NF1 to the cell membrane, thereby enabling NF1-dependent hydrolysis of active GTP-bound Ras to inactive GDP-bound Ras (By similarity).
Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2 (PubMed:29501879).
Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells (PubMed:25576668).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasmic vesicle | |
Cellular Component | plasma membrane | |
Cellular Component | transport vesicle membrane | |
Molecular Function | protein kinase binding | |
Molecular Function | protein serine/threonine kinase inhibitor activity | |
Molecular Function | stem cell factor receptor binding | |
Biological Process | negative regulation of epithelial to mesenchymal transition | |
Biological Process | negative regulation of ERK1 and ERK2 cascade | |
Biological Process | negative regulation of lens fiber cell differentiation | |
Biological Process | negative regulation of MAPK cascade | |
Biological Process | negative regulation of peptidyl-threonine phosphorylation | |
Biological Process | negative regulation of transforming growth factor beta receptor signaling pathway | |
Biological Process | positive regulation of DNA damage response, signal transduction by p53 class mediator | |
Biological Process | regulation of protein deacetylation |
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSprouty-related, EVH1 domain-containing protein 2
- Short namesSpred-2
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ924S7
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Cytoplasmic vesicle, secretory vesicle membrane ; Peripheral membrane protein
Note: Detected in the cytoplasm of the stratum spinosum cells, where it is associated with cytoplasmic vesicles that are supposed to be secretory granules.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
SPRED2 knockout results in a dwarf phenotype characterized by reduced growth and body weight, shorter tibia length, and narrower growth plates as compared with wild-type mice. Mutant animals regularly develop kyphosis and scoliosis, and show craniofacial defects, splenomegaly, and cardiac hypertrophy with arrhythmias.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 27 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000076911 | 1-410 | Sprouty-related, EVH1 domain-containing protein 2 | |||
Sequence: MTEETHPDDDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIHGERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIEDLIEGSTTSSSTLHNEAELGDDDVFTTATDSSSNSSQKREPTTRTISSPTSCEHRKIYTLDPYPMDHYHPDQRLPRSYPQVTFPEDDEEIVRINPREKIWMTGYEDYRHAPVRGKYLDTTEDADSYVRFAKGEVPKHEYTYPYVDSSDFGFGEDPKGSVIKTQPPRAKSRRRKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACHRCGVMCRCCGGKHKAAA | ||||||
Modified residue | 224 | Phosphotyrosine | ||||
Sequence: Y | ||||||
Modified residue | 227 | Phosphotyrosine | ||||
Sequence: Y |
Post-translational modification
Phosphorylated on serine and threonine residues. Phosphorylated on tyrosine. Phosphorylation of Tyr-224 and Tyr-227 are required for ubiquitination.
Ubiquitinated; leading to degradation by the proteasome.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Predominantly expressed in lung, liver and testis. In testis, it is specially found in mature spermatids projecting into the lumen of the seminiferous. Strongly expressed in glandular epithelia. Also expressed in embryonic tissues such as heart, lung, liver and brain.
Gene expression databases
Interaction
Subunit
Homodimer and heterodimer (By similarity).
Able to interact with SPRED1 to form heterodimers (By similarity).
Interacts with RAS (PubMed:11493923).
May interact with ZDHHC13 (via ANK repeats) and ZDHHC17 (via ANK repeats) (PubMed:28882895).
Interacts with TESK1 (PubMed:17974561).
Interacts with NF1 (By similarity).
Able to interact with SPRED1 to form heterodimers (By similarity).
Interacts with RAS (PubMed:11493923).
May interact with ZDHHC13 (via ANK repeats) and ZDHHC17 (via ANK repeats) (PubMed:28882895).
Interacts with TESK1 (PubMed:17974561).
Interacts with NF1 (By similarity).
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 5-122 | WH1 | ||||
Sequence: THPDDDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIHGERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIEDL | ||||||
Region | 127-171 | Disordered | ||||
Sequence: TTSSSTLHNEAELGDDDVFTTATDSSSNSSQKREPTTRTISSPTS | ||||||
Compositional bias | 145-171 | Polar residues | ||||
Sequence: FTTATDSSSNSSQKREPTTRTISSPTS | ||||||
Domain | 197-252 | KBD | ||||
Sequence: SYPQVTFPEDDEEIVRINPREKIWMTGYEDYRHAPVRGKYLDTTEDADSYVRFAKG | ||||||
Region | 274-294 | Disordered | ||||
Sequence: DPKGSVIKTQPPRAKSRRRKE | ||||||
Domain | 300-408 | SPR | ||||
Sequence: RCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSCMWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACHRCGVMCRCCGGKHKA |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length410
- Mass (Da)46,794
- Last updated2001-12-01 v1
- Checksum8A652D06CE8D3BB1
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0AAQ4VMS6 | A0AAQ4VMS6_MOUSE | Spred2 | 407 | ||
Q5SRF8 | Q5SRF8_MOUSE | Spred2 | 437 | ||
Q5SRF9 | Q5SRF9_MOUSE | Spred2 | 229 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 145-171 | Polar residues | ||||
Sequence: FTTATDSSSNSSQKREPTTRTISSPTS |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB063496 EMBL· GenBank· DDBJ | BAB62849.1 EMBL· GenBank· DDBJ | mRNA | ||
AK036430 EMBL· GenBank· DDBJ | BAC29425.1 EMBL· GenBank· DDBJ | mRNA | ||
BC066013 EMBL· GenBank· DDBJ | AAH66013.1 EMBL· GenBank· DDBJ | mRNA |