Q92331 · VPS5_YEAST
- ProteinVacuolar protein sorting-associated protein 5
- GeneVPS5
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids675 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plays a role in vesicular protein sorting. Required for retention of late Golgi membrane proteins and vacuolar biogenesis. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS5-VPS17 subcomplex may assemble onto the membrane to promote vesicle formation.
Miscellaneous
Present with 6120 molecules/cell in log phase SD medium.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 320 | a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 346 | a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 360 | a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) (UniProtKB | ChEBI) | ||||
Sequence: R |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | endomembrane system | |
Cellular Component | endosome | |
Cellular Component | endosome membrane | |
Cellular Component | Golgi membrane | |
Cellular Component | retromer complex | |
Cellular Component | retromer, tubulation complex | |
Molecular Function | phosphatidylinositol binding | |
Molecular Function | phosphatidylinositol-3-phosphate binding | |
Biological Process | endocytic recycling | |
Biological Process | protein retention in Golgi apparatus | |
Biological Process | protein transport | |
Biological Process | retrograde transport, endosome to Golgi |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameVacuolar protein sorting-associated protein 5
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionQ92331
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Peripheral membrane protein
Endosome membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000213804 | 1-675 | Vacuolar protein sorting-associated protein 5 | |||
Sequence: MDYEDNLEAPVWDELNHEGDKTQSLVSNSIESIGQISTNEERKDNELLETTASFADKIDLDSAPEWKDPGLSVAGNPQLEEHDNSKADDLINSLAPEQDPIADLKNSTTQFIATRESGGALFTGNANSPLVFDDTIYDANTSPNTSKSISGRRSGKPRILFDSARAQRNSKRNHSLKAKRTTASDDTIKTPFTDPLKKAEKENEFVEEPLDDRNERRENNEGKFTASVEKNILEQVDRPLYNLPQTGANISSPAEVEENSEKFGKTKIGSKVPPTEKAVAFKVEVKDPVKVGELTSIHVEYTVISESSLLELKYAQVSRRYRDFRWLYRQLQNNHWGKVIPPPPEKQSVGSFKENFIENRRFQMESMLKKICQDPVLQKDKDFLLFLTSDDFSSESKKRAFLTGSGAINDSNDLSEVRISEIQLLGAEDAAEVLKNGGIDAESHKGFMSISFSSLPKYNEADEFFIEKKQKIDELEDNLKKLSKSLEMVDTSRNTLAASTEEFSSMVETLASLNVSEPNSELLNNFADVHKSIKSSLERSSLQETLTMGVMLDDYIRSLASVKAIFNQRSKLGYFLVVIENDMNKKHSQLGKLGQNIHSEKFREMRKEFQTLERRYNLTKKQWQAVGDKIKDEFQGFSTDKIREFRNGMEISLEAAIESQKECIELWETFYQTNL |
Post-translational modification
Phosphorylated on serine residue(s).
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Component of the retromer complex which consists of VPS29, VPS26, VPS35, VPS5 and VPS17. Component of a retromer subcomplex consisting of VPSD5 and VPS17.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q92331 | PEP8 P40335 | 3 | EBI-20483, EBI-20373 | |
BINARY | Q92331 | VPS17 P32913 | 8 | EBI-20483, EBI-20366 | |
BINARY | Q92331 | VPS35 P34110 | 4 | EBI-20483, EBI-20415 |
Complex viewer
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-26 | Disordered | ||||
Sequence: MDYEDNLEAPVWDELNHEGDKTQSLV | ||||||
Region | 65-84 | Disordered | ||||
Sequence: EWKDPGLSVAGNPQLEEHDN | ||||||
Region | 165-219 | Disordered | ||||
Sequence: RAQRNSKRNHSLKAKRTTASDDTIKTPFTDPLKKAEKENEFVEEPLDDRNERREN | ||||||
Compositional bias | 191-219 | Basic and acidic residues | ||||
Sequence: PFTDPLKKAEKENEFVEEPLDDRNERREN | ||||||
Domain | 279-394 | PX | ||||
Sequence: VAFKVEVKDPVKVGELTSIHVEYTVISESSLLELKYAQVSRRYRDFRWLYRQLQNNHWGKVIPPPPEKQSVGSFKENFIENRRFQMESMLKKICQDPVLQKDKDFLLFLTSDDFSS |
Domain
The PX domain binds phosphatidylinositol 3-phosphate which is necessary for peripheral membrane localization.
Sequence similarities
Belongs to the sorting nexin family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length675
- Mass (Da)76,484
- Last updated1997-02-01 v1
- ChecksumFBBA994EADBDC2BD
Sequence caution
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 191-219 | Basic and acidic residues | ||||
Sequence: PFTDPLKKAEKENEFVEEPLDDRNERREN | ||||||
Sequence conflict | 376 | in Ref. 6; AAS56361 | ||||
Sequence: V → A |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U73512 EMBL· GenBank· DDBJ | AAB62976.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U84735 EMBL· GenBank· DDBJ | AAB41798.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z70678 EMBL· GenBank· DDBJ | CAA94554.1 EMBL· GenBank· DDBJ | Genomic DNA | Frameshift | |
Z74976 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
Z74977 EMBL· GenBank· DDBJ | CAA99262.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
AY558035 EMBL· GenBank· DDBJ | AAS56361.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006948 EMBL· GenBank· DDBJ | DAA10847.1 EMBL· GenBank· DDBJ | Genomic DNA |