Q921N6 · DDX27_MOUSE
- ProteinProbable ATP-dependent RNA helicase DDX27
- GeneDdx27
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids760 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Probable ATP-dependent RNA helicase. Component of the nucleolar ribosomal RNA (rRNA) processing machinery that regulates 3' end formation of ribosomal 47S rRNA.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome | |
Cellular Component | nucleolus | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | nucleic acid binding | |
Molecular Function | RNA helicase activity | |
Biological Process | rRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable ATP-dependent RNA helicase DDX27
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ921N6
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Associates with 60S and 90S pre-ribosomal particles.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000055032 | 1-760 | Probable ATP-dependent RNA helicase DDX27 | |||
Sequence: MLAELGFIRTIGENDEVPVEPESDSGDEEEEGPIVLGRKQKALQKNRSADFNPDFVFTEKEGMYDGSWALADVMSQLKKKRAATTLDEKIEKVRKRRKAEDKEAKSGKVEEKEGQADSDLKGQENPGEDEAGSKDEDSETDYSSEDEEILTKADTLKVKEKKKKKKGQAAGGFFEDASEYDKSLSFQDMNLSRPLLKAITAMGFKQPTPIQKACIPVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQAAVTRVLVLVPTRELGIQVHSVTKQLAQFCSITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSIEVLILDEADRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRIFVNSNTDVAPFLRQEFIRIRPNREGDREAIVAALLMRTFTDHVMLFTQTKKQAHRMHILLGLLGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPNTVKHYVHRVGRTARAGRAGRSVSLVGEEERKMLKEIVKAAKAPVKARILPQDVILKFRDKIEKLEKDVYAVLQLEAEEKEMQQSEAQIDTAQRLLAKGKETADQEPERSWFQTKEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAERLAKRNRRTKRARAMPEDEPTGPAKKQKQQQKSVFDEELTNTSKKALKQYRAGPSFEERKQSGLPRQRRGNFKSKSRYKRKK | ||||||
Modified residue | 23 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 25 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 48 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 133 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 144 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, motif, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-50 | Disordered | ||||
Sequence: MLAELGFIRTIGENDEVPVEPESDSGDEEEEGPIVLGRKQKALQKNRSAD | ||||||
Motif | 55-57 | Required for interaction with the PEBOW complex | ||||
Sequence: FVF | ||||||
Region | 80-149 | Disordered | ||||
Sequence: KRAATTLDEKIEKVRKRRKAEDKEAKSGKVEEKEGQADSDLKGQENPGEDEAGSKDEDSETDYSSEDEEI | ||||||
Compositional bias | 81-129 | Basic and acidic residues | ||||
Sequence: RAATTLDEKIEKVRKRRKAEDKEAKSGKVEEKEGQADSDLKGQENPGED | ||||||
Compositional bias | 130-144 | Acidic residues | ||||
Sequence: EAGSKDEDSETDYSS | ||||||
Motif | 157-166 | Nuclear localization signal | ||||
Sequence: KVKEKKKKKK | ||||||
Motif | 184-212 | Q motif | ||||
Sequence: LSFQDMNLSRPLLKAITAMGFKQPTPIQK | ||||||
Domain | 215-389 | Helicase ATP-binding | ||||
Sequence: IPVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQAAVTRVLVLVPTRELGIQVHSVTKQLAQFCSITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSIEVLILDEADRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRI | ||||||
Motif | 337-340 | DEAD box | ||||
Sequence: DEAD | ||||||
Domain | 419-569 | Helicase C-terminal | ||||
Sequence: IVAALLMRTFTDHVMLFTQTKKQAHRMHILLGLLGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPNTVKHYVHRVGRTARAGRAGRSVSLVGEEERKMLKEIVKAAKAPVKARILPQDVILKFRDKI | ||||||
Region | 605-624 | Disordered | ||||
Sequence: KGKETADQEPERSWFQTKEE | ||||||
Region | 679-760 | Disordered | ||||
Sequence: RLAKRNRRTKRARAMPEDEPTGPAKKQKQQQKSVFDEELTNTSKKALKQYRAGPSFEERKQSGLPRQRRGNFKSKSRYKRKK | ||||||
Compositional bias | 689-722 | Basic and acidic residues | ||||
Sequence: RARAMPEDEPTGPAKKQKQQQKSVFDEELTNTSK | ||||||
Compositional bias | 729-743 | Basic and acidic residues | ||||
Sequence: RAGPSFEERKQSGLP |
Domain
The C-terminal domain regulates nucleolar localization.
Sequence similarities
Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q921N6-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length760
- Mass (Da)85,939
- Last updated2011-07-27 v3
- ChecksumC351B13DAC1B1E0B
Q921N6-2
- Name2
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
H3BLJ6 | H3BLJ6_MOUSE | Ddx27 | 310 |
Sequence caution
Features
Showing features for compositional bias, alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 81-129 | Basic and acidic residues | ||||
Sequence: RAATTLDEKIEKVRKRRKAEDKEAKSGKVEEKEGQADSDLKGQENPGED | ||||||
Compositional bias | 130-144 | Acidic residues | ||||
Sequence: EAGSKDEDSETDYSS | ||||||
Alternative sequence | VSP_007073 | 292 | in isoform 2 | |||
Sequence: G → E | ||||||
Alternative sequence | VSP_007074 | 293-760 | in isoform 2 | |||
Sequence: Missing | ||||||
Sequence conflict | 341 | in Ref. 4; AAH11321 | ||||
Sequence: R → K | ||||||
Compositional bias | 689-722 | Basic and acidic residues | ||||
Sequence: RARAMPEDEPTGPAKKQKQQQKSVFDEELTNTSK | ||||||
Compositional bias | 729-743 | Basic and acidic residues | ||||
Sequence: RAGPSFEERKQSGLP |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK134274 EMBL· GenBank· DDBJ | BAE22077.1 EMBL· GenBank· DDBJ | mRNA | ||
AK138442 EMBL· GenBank· DDBJ | BAE23664.1 EMBL· GenBank· DDBJ | mRNA | ||
AL591711 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH466551 EMBL· GenBank· DDBJ | EDL06495.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC011321 EMBL· GenBank· DDBJ | AAH11321.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
BC024730 EMBL· GenBank· DDBJ | AAH24730.1 EMBL· GenBank· DDBJ | mRNA | ||
BC026381 EMBL· GenBank· DDBJ | AAH26381.1 EMBL· GenBank· DDBJ | mRNA |