Q920Q4 · VPS16_MOUSE
- ProteinVacuolar protein sorting-associated protein 16 homolog
- GeneVps16
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids839 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for recruitment of VPS33A to the HOPS complex. Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS33A but not VPS33B. The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | actin filament | |
Cellular Component | autophagosome | |
Cellular Component | axon | |
Cellular Component | clathrin-coated vesicle | |
Cellular Component | CORVET complex | |
Cellular Component | early endosome | |
Cellular Component | early endosome membrane | |
Cellular Component | endosome | |
Cellular Component | HOPS complex | |
Cellular Component | late endosome membrane | |
Cellular Component | lysosomal membrane | |
Cellular Component | neuronal cell body | |
Cellular Component | presynapse | |
Molecular Function | actin binding | |
Molecular Function | actin filament binding | |
Biological Process | autophagy | |
Biological Process | endosomal transport | |
Biological Process | intracellular protein transport | |
Biological Process | symbiont entry into host cell | |
Biological Process | vacuole fusion, non-autophagic |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameVacuolar protein sorting-associated protein 16 homolog
- Short namesmVPS16
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ920Q4
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Late endosome membrane ; Peripheral membrane protein
Lysosome membrane ; Peripheral membrane protein
Note: Colocalizes with AP-3, clathrin, Rab5 and Rab7b (PubMed:21411634).
Cytoplasmic, peripheral membrane protein associated with early endosomes and late endosomes/lysosomes
Cytoplasmic, peripheral membrane protein associated with early endosomes and late endosomes/lysosomes
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 52 | Down-regulation of VPS16 expression in mice; mice exhibited obvious abnormality in the behavior and significantly impaired motor function. | ||||
Sequence: N → K |
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000065889 | 1-839 | Vacuolar protein sorting-associated protein 16 homolog | |||
Sequence: MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELKDCLVAAAPYGGPIALLRNCWRKEKAASVRPVLEIYSASGLPLASLLWKSGPVVALGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAYRFTLSANVGDLKLRRMPEVPGLQSAPSCWTTLCHDRVPHILLAVGPDLYLLDHATCSAVTPAGLAPGVSSFLQMAVSFTYRYLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGNAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPVASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLIQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFVHMCQDLRVLNAIRDYHIGIPLTYTQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKARAWDMRDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELDTLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRIQRRLEDELGGRFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLAALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKHEAKKYASRVGPEQKVKALLLVGDVAQAAEVAIEHRNETELSLVLSHCTGATDGAIADKIQRARAQAQKK | ||||||
Modified residue | 4 | 3'-nitrotyrosine | ||||
Sequence: Y |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuole/endosome tethering (CORVET) complex. Their common core is composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1 (PubMed:25266290).
Interacts with RAB5C (PubMed:25266290).
Interacts with STX17, MON1B (By similarity).
Associates with adapter protein complex 3 (AP-3) and clathrin:AP-3 complexes (PubMed:21411634).
Interacts with RAB5C (PubMed:25266290).
Interacts with STX17, MON1B (By similarity).
Associates with adapter protein complex 3 (AP-3) and clathrin:AP-3 complexes (PubMed:21411634).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
XENO | Q920Q4 | VIPAS39 Q9H9C1 | 4 | EBI-775797, EBI-749080 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 642-736 | Interaction with VPS33A | ||||
Sequence: ERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRIQRRLEDELGGRFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLAALAD |
Sequence similarities
Belongs to the VPS16 family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q920Q4-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length839
- Mass (Da)94,928
- Last updated2011-07-27 v3
- Checksum42C9B3C87E5763C2
Q920Q4-2
- Name2
- Differences from canonical
- 1-420: Missing
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_004019 | 1-420 | in isoform 2 | |||
Sequence: Missing | ||||||
Sequence conflict | 155 | in Ref. 1; BAB64892 | ||||
Sequence: L → Q | ||||||
Sequence conflict | 173 | in Ref. 1; BAB64892 and 3; AAH25626 | ||||
Sequence: L → M | ||||||
Sequence conflict | 431 | in Ref. 1; BAB64892 | ||||
Sequence: I → V | ||||||
Sequence conflict | 493-499 | in Ref. 1; BAB64892 and 3; AAH03944/AAH25626 | ||||
Sequence: ARAWDMR → VQQKDVS | ||||||
Sequence conflict | 653 | in Ref. 3; AAH25626 | ||||
Sequence: T → M |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB056721 EMBL· GenBank· DDBJ | BAB64892.1 EMBL· GenBank· DDBJ | mRNA | ||
BX890605 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC003944 EMBL· GenBank· DDBJ | AAH03944.1 EMBL· GenBank· DDBJ | mRNA | ||
BC025626 EMBL· GenBank· DDBJ | AAH25626.1 EMBL· GenBank· DDBJ | mRNA |