Q920Q4 · VPS16_MOUSE

  • Protein
    Vacuolar protein sorting-associated protein 16 homolog
  • Gene
    Vps16
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for recruitment of VPS33A to the HOPS complex. Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS33A but not VPS33B. The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentactin filament
Cellular Componentautophagosome
Cellular Componentaxon
Cellular Componentclathrin-coated vesicle
Cellular ComponentCORVET complex
Cellular Componentearly endosome
Cellular Componentearly endosome membrane
Cellular Componentendosome
Cellular ComponentHOPS complex
Cellular Componentlate endosome membrane
Cellular Componentlysosomal membrane
Cellular Componentneuronal cell body
Cellular Componentpresynapse
Molecular Functionactin binding
Molecular Functionactin filament binding
Biological Processautophagy
Biological Processendosomal transport
Biological Processintracellular protein transport
Biological Processsymbiont entry into host cell
Biological Processvacuole fusion, non-autophagic

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Vacuolar protein sorting-associated protein 16 homolog
  • Short names
    mVPS16

Gene names

    • Name
      Vps16

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q920Q4
  • Secondary accessions
    • A2BI91
    • Q8R3C3
    • Q99KZ4

Proteomes

Organism-specific databases

Subcellular Location

Late endosome membrane
; Peripheral membrane protein
Lysosome membrane
; Peripheral membrane protein
Early endosome
Note: Colocalizes with AP-3, clathrin, Rab5 and Rab7b (PubMed:21411634).
Cytoplasmic, peripheral membrane protein associated with early endosomes and late endosomes/lysosomes

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis52Down-regulation of VPS16 expression in mice; mice exhibited obvious abnormality in the behavior and significantly impaired motor function.

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000658891-839Vacuolar protein sorting-associated protein 16 homolog
Modified residue43'-nitrotyrosine

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuole/endosome tethering (CORVET) complex. Their common core is composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1 (PubMed:25266290).
Interacts with RAB5C (PubMed:25266290).
Interacts with STX17, MON1B (By similarity).
Associates with adapter protein complex 3 (AP-3) and clathrin:AP-3 complexes (PubMed:21411634).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
XENO Q920Q4VIPAS39 Q9H9C14EBI-775797, EBI-749080

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region642-736Interaction with VPS33A

Sequence similarities

Belongs to the VPS16 family.

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q920Q4-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    839
  • Mass (Da)
    94,928
  • Last updated
    2011-07-27 v3
  • Checksum
    42C9B3C87E5763C2
MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELKDCLVAAAPYGGPIALLRNCWRKEKAASVRPVLEIYSASGLPLASLLWKSGPVVALGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAYRFTLSANVGDLKLRRMPEVPGLQSAPSCWTTLCHDRVPHILLAVGPDLYLLDHATCSAVTPAGLAPGVSSFLQMAVSFTYRYLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGNAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPVASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLIQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFVHMCQDLRVLNAIRDYHIGIPLTYTQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKARAWDMRDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELDTLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRIQRRLEDELGGRFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLAALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKHEAKKYASRVGPEQKVKALLLVGDVAQAAEVAIEHRNETELSLVLSHCTGATDGAIADKIQRARAQAQKK

Q920Q4-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A2BI90A2BI90_MOUSEVps16316
G3X8X7G3X8X7_MOUSEVps16839

Features

Showing features for alternative sequence, sequence conflict.

TypeIDPosition(s)Description
Alternative sequenceVSP_0040191-420in isoform 2
Sequence conflict155in Ref. 1; BAB64892
Sequence conflict173in Ref. 1; BAB64892 and 3; AAH25626
Sequence conflict431in Ref. 1; BAB64892
Sequence conflict493-499in Ref. 1; BAB64892 and 3; AAH03944/AAH25626
Sequence conflict653in Ref. 3; AAH25626

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB056721
EMBL· GenBank· DDBJ
BAB64892.1
EMBL· GenBank· DDBJ
mRNA
BX890605
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC003944
EMBL· GenBank· DDBJ
AAH03944.1
EMBL· GenBank· DDBJ
mRNA
BC025626
EMBL· GenBank· DDBJ
AAH25626.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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