Q91Z67 · SRGP2_MOUSE

  • Protein
    SLIT-ROBO Rho GTPase-activating protein 2
  • Gene
    Srgap2
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Postsynaptic RAC1 GTPase activating protein (GAP) that plays a key role in neuronal morphogenesis and migration mainly during development of the cerebral cortex (PubMed:19737524, PubMed:22559944, PubMed:27373832).
Regulates excitatory and inhibitory synapse maturation and density in cortical pyramidal neurons (PubMed:19737524, PubMed:22559944, PubMed:27373832).
SRGAP2/SRGAP2A limits excitatory and inhibitory synapse density through its RAC1-specific GTPase activating activity, while it promotes maturation of both excitatory and inhibitory synapses through its ability to bind to the postsynaptic scaffolding protein HOMER1 at excitatory synapses, and the postsynaptic protein GPHN at inhibitory synapses (PubMed:27373832).
Mechanistically, acts by binding and deforming membranes, thereby regulating actin dynamics to regulate cell migration and differentiation (PubMed:19737524, PubMed:22559944, PubMed:26439400).
Promotes cell repulsion and contact inhibition of locomotion: localizes to protrusions with curved edges and controls the duration of RAC1 activity in contact protrusions (PubMed:26439400).
In non-neuronal cells, may also play a role in cell migration by regulating the formation of lamellipodia and filopodia (PubMed:22559944).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentdendritic spine
Cellular Componentdendritic spine head
Cellular Componentglutamatergic synapse
Cellular Componentlamellipodium
Cellular Componentmitochondrial inner membrane
Cellular Componentnucleus
Cellular Componentphagocytic vesicle
Cellular Componentplasma membrane
Cellular Componentpostsynaptic density
Cellular Componentpostsynaptic membrane
Molecular FunctionGTPase activator activity
Molecular Functionprotein homodimerization activity
Molecular Functionsmall GTPase binding
Biological Processactin filament severing
Biological Processdendritic spine development
Biological Processexcitatory synapse assembly
Biological Processextension of a leading process involved in cell motility in cerebral cortex radial glia guided migration
Biological Processfilopodium assembly
Biological Processinhibitory synapse assembly
Biological Processlamellipodium assembly involved in ameboidal cell migration
Biological Processnegative regulation of cell migration
Biological Processnegative regulation of neuron migration
Biological Processneuron projection morphogenesis
Biological Processpositive regulation of GTPase activity
Biological Processregulation of cell migration
Biological Processregulation of synapse assembly
Biological Processsignal transduction
Biological Processsubstrate adhesion-dependent cell spreading

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    SLIT-ROBO Rho GTPase-activating protein 2
  • Short names
    srGAP2
  • Alternative names
    • Formin-binding protein 2
    • Formin-binding protein 27
      (FBP-27
      )

Gene names

    • Name
      Srgap2
    • Synonyms
      Fbp27
      , Fnbp2

Organism names

  • Taxonomic identifier
  • Strains
    • C57BL/6J
    • FVB/NJ
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q91Z67
  • Secondary accessions
    • B2RY13
    • Q3V1V8
    • Q61054

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
Postsynaptic density
Nucleus
Cytoplasm, cytosol
Note: Recruited to actin-rich phagosomes during phagocytosis (PubMed:21148482).
Translocates from nucleus to cytoplasm during development (By similarity).

Keywords

Phenotypes & Variants

Disruption phenotype

Mice are viable and show no abnormality of cortical lamination. However, a delay in dendritic spine maturation coupled to an increase in spine neck and spine density is observed.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis340-343Abolished interaction with HOMER1.
Mutagenesis527Abolished RAC1 GTPase activity. Abolished ability to induce neurite branching. No effect on filopodia biogenesis and neurite outgrowth.
Mutagenesis765Loss of the ability to induce filopodia and to initiate neurite outgrowth. Abolished interaction with GPHN.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 34 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000567681-1071SLIT-ROBO Rho GTPase-activating protein 2
Modified residue206Phosphoserine
Modified residue427Phosphoserine
Modified residue500Phosphoserine
Modified residue691Phosphoserine
Modified residue695Phosphoserine
Modified residue724Phosphoserine
Modified residue795Phosphoserine
Modified residue916Phosphoserine
Modified residue927Symmetric dimethylarginine; by PRMT5
Modified residue930Phosphoserine
Modified residue990Phosphoserine
Modified residue994Phosphoserine
Modified residue1013Phosphoserine
Modified residue1027Phosphoserine

Post-translational modification

Methylation at Arg-927 is required for the stimulation of cell migration, dimerization and localization at the plasma membrane protrusions.

Keywords

Proteomic databases

PTM databases

Expression

Developmental stage

Expressed throughout cortical development culminating at P1. Expression is reduced but still present in the adult cortex. Expressed in the cortical wall both in neuronal progenitors in the ventricular zone and post-mitotic neurons in the cortical plate (at protein level).

Gene expression databases

Interaction

Subunit

Homodimer (PubMed:19737524).
Forms a heterooligomer with SRGAP1 and SRGAP3 through its F-BAR domain (By similarity).
Interacts (via SH3 domain) with GPHN (PubMed:22126966, PubMed:27373832).
Interacts (via SH3 domain) with FMNL1 (activated by RAC1); regulates the actin filament severing activity of FMNL1 and actin dynamics (PubMed:21148482).
Interacts (via SH3 domain) with FMNL3 (PubMed:21148482).
Interacts with RAC1; specifically stimulates RAC1 GTPase activity (PubMed:19737524).
Interacts (via F-BAR domain) with HOMER1 (PubMed:27373832).
Interacts with ROBO1 and ROBO2 (PubMed:21148482).
Interacts with FASLG (By similarity).
Interacts with PRMT5 (By similarity).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for domain, compositional bias, region, coiled coil.

TypeIDPosition(s)Description
Domain22-325F-BAR
Compositional bias181-204Basic and acidic residues
Region181-211Disordered
Coiled coil363-401
Domain489-679Rho-GAP
Region700-726Disordered
Domain728-787SH3
Compositional bias795-809Basic and acidic residues
Region795-819Disordered
Region838-918Disordered
Compositional bias855-884Polar residues
Compositional bias896-918Polar residues
Coiled coil940-968
Region984-1012Disordered
Region1029-1071Disordered
Compositional bias1045-1071Polar residues

Domain

The F-BAR domain mediates oligomerization, binds membranes, and induces plasma membrane protrusions.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,071
  • Mass (Da)
    120,798
  • Last updated
    2007-01-09 v2
  • Checksum
    9093C63476BA605D
MTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPRFVQVSEDSGRLFKKSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDSTANVRIEEKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDIIDQCCDLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMGDMASQLCAQQPVQSELVQRCQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSMESVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGESQRTDCSLARRSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLEHPLFPKDIFHDLIACVTMDNLQERAVHIRKVLLVLPKPTLIIMRYLFAFLNHLSQFSEENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPNPRELEGPIYSRGGSMEDYCDSTHGETTSAEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRHNGIDGLIPHQYIVVQDTEDGVVERSSPKSEIEVMSEPPEEKVTARTGASCPSGGHVADIYLANINKQRKRPESGSIRKAFRSDSHGLGSSLTDSSSLGVGASCRPSSQPIMSQNLPKEGPDKCSISGHGSLNSISRHSSLKNRMDSPQIRKTATAGRSKSFNNHRPMDPEVIAQDIEATMNSALNELQELERQSSAKHTPDVVLDTLEPLKTSPVVAPTSEPSSPLHTQLLKDPEPAFQRSASTAGDIACAFRPVKSVKMAAPVKPPATRPKPTVFPKTNATSPGVNSSASPQATDKSCTV

Computationally mapped potential isoform sequences

There are 6 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A087WNM1A0A087WNM1_MOUSESrgap2858
A0A087WNR5A0A087WNR5_MOUSESrgap255
A0A087WS59A0A087WS59_MOUSESrgap2451
A0A087WRV4A0A087WRV4_MOUSESrgap2110
A0A087WS73A0A087WS73_MOUSESrgap2267
A0A087WSQ1A0A087WSQ1_MOUSESrgap2649

Features

Showing features for compositional bias, sequence conflict.

TypeIDPosition(s)Description
Compositional bias181-204Basic and acidic residues
Sequence conflict598in Ref. 3; AAL27032
Sequence conflict612in Ref. 3; AAL27032
Sequence conflict662in Ref. 3; AAL27032
Sequence conflict737in Ref. 3; AAL27032
Sequence conflict765in Ref. 3; AAL27032
Compositional bias795-809Basic and acidic residues
Compositional bias855-884Polar residues
Compositional bias896-918Polar residues
Compositional bias1045-1071Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC109299
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AC120217
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AC165436
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC151081
EMBL· GenBank· DDBJ
AAI51082.1
EMBL· GenBank· DDBJ
mRNA
BC151082
EMBL· GenBank· DDBJ
AAI51083.1
EMBL· GenBank· DDBJ
mRNA
BC158055
EMBL· GenBank· DDBJ
AAI58056.1
EMBL· GenBank· DDBJ
mRNA
BC172152
EMBL· GenBank· DDBJ
AAI72152.1
EMBL· GenBank· DDBJ
mRNA
AY057900
EMBL· GenBank· DDBJ
AAL27032.1
EMBL· GenBank· DDBJ
mRNA
AK132220
EMBL· GenBank· DDBJ
BAE21041.1
EMBL· GenBank· DDBJ
mRNA
U40752
EMBL· GenBank· DDBJ
AAC52480.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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